本文選題：丙烯酰胺 + 神經發(fā)育毒性�。� 參考：《神經解剖學雜志》2017年04期

【摘要】：目的:探討母源性丙烯酰胺(acrylamide,ACR)暴露對子代鼠大腦皮質神經元MAP-2與氧化應激的影響。方法:SD孕鼠隨機分為4組,自懷孕第6 d至懷孕第19 d起對照組和實驗組分別灌胃給藥給予雙蒸水和每日8,16和24 mg/kg ACR溶液。HE和尼氏染色觀察子代鼠皮質神經元形態(tài)學改變,免疫組織化學和Western Blot檢測子代鼠皮質神經元MAP-2的表達,應用試劑盒檢測大腦皮質組織中氧化應激指標丙二醛(MDA)、總超氧化物歧化酶(T-SOD)、還原谷胱甘肽(GSH)。結果:(1)MAP-2表達:與對照組相比,實驗組中、高劑量組MAP-2表達下降,差異有統(tǒng)計學意義(P0.05),而低劑量組則差異不顯著(P0.05)。(2)氧化應激指標變化:與對照組相比,實驗組中、高劑量組子代鼠大腦皮質組織中MDA含量顯著升高,而T-SOD活力和GSH含量顯著下降,差異具有統(tǒng)計學意義(P0.05)。結論:丙烯酰胺致子代鼠神經元損傷的機制可能與其誘導氧化應激損傷有關。
[Abstract]:Aim: to investigate the effects of exposure to maternal acrylamide (ACR) on MAP-2 and oxidative stress in cerebral cortex neurons of offspring rats. Methods Twenty SD pregnant rats were randomly divided into 4 groups. From the sixth day of pregnancy to the 19th day of pregnancy, the control group and the experimental group were given double steamed water and 816 and 24 mg/kg ACR solution respectively. The morphological changes of cortex neurons were observed by HE and Nissl staining. Immunohistochemistry and Western Blot were used to detect the expression of MAP-2 in cortical neurons of offspring rats. The oxidative stress index of malondialdehyde (MDA) in cerebral cortex, total superoxide dismutase (T-SOD), and reduced glutathione (GSH) were detected by kit. Results: compared with the control group, the expression of MAP-2 in the high dose group was significantly lower than that in the control group, but the difference was not significant in the low dose group. The content of MDA in cerebral cortex of offspring of high dose group was significantly increased, while the activity of T-SOD and the content of GSH were decreased significantly. The difference was statistically significant (P 0.05). Conclusion: the mechanism of neuronal injury induced by acrylamide may be related to oxidative stress injury induced by acrylamide.
【作者單位】： 肇慶醫(yī)學高等專科學校;
【基金】：廣東省醫(yī)學科學基金項目(A201618) 廣東省中醫(yī)藥局中醫(yī)藥常規(guī)科研項目(20162156) 肇慶市科技創(chuàng)新指導項目(2014E184) 肇慶市醫(yī)學高等專科學校青年科技基金(zqyq16-001)
【分類號】：R114

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