本文選題：沙海蜇 + 光老化�。� 參考：《中國海洋大學(xué)》2012年碩士論文

【摘要】：1本文以沙海蜇皮為研究對象，討論了腌漬沙海蜇皮的基本組成，從其基本組成可知腌漬沙海蜇皮中最主要的成分是水分和鹽分，在干物質(zhì)中最主要的成分是蛋白質(zhì)，其中蛋白質(zhì)中膠原蛋白約占到三分之一，腌漬沙海蜇中高水分、高鹽分，較低的膠原蛋白的純度為其中的膠原蛋白的提取增加了難度，需要尋找一種有效的提取方式對沙海蜇中的膠原蛋白進(jìn)行大規(guī)模的提取以進(jìn)行后續(xù)實(shí)驗(yàn)。因此本文討論了提取膠原蛋白常用的三種方法（酶法提取、熱水提取和酸法提�。┧萌N膠原蛋白PSC（酶溶性蛋白），HEG（熱水提取膠原蛋白），ASC（酸溶性膠原蛋白）的理化性質(zhì)。結(jié)果表明三種方法得到的膠原蛋白結(jié)構(gòu)相似。酸法提取可以得到純度較高的膠原蛋白，其羥脯氨酸含量高于PSC和HEG，并且ASC具有良好的乳化穩(wěn)定性。HEG分子量相對較大，且具有良好的乳化性。其吸濕性由強(qiáng)到弱依次為PSCHEGASC，保濕性最好的樣品為PSC。綜合各種因素的考量，本實(shí)驗(yàn)最終選用熱水對膠原蛋白進(jìn)行大規(guī)模的提取。并將所得的膠原蛋白進(jìn)一步的酶解，超濾成不同分子量的肽段。 2以紫外誘導(dǎo)光老化的小鼠皮膚為研究對象，研究了海蜇膠原蛋白對其皮膚的保護(hù)作用。實(shí)驗(yàn)小鼠共分為11組，即正常對照組，模型對照組，陽性對照組，膠原蛋白高劑量組以及膠原蛋白低劑量組，Mr8000高、低劑量組，2500Mr8000高、低劑量組，Mr2500高、低劑量組。通過對小鼠皮膚與血清中生化指標(biāo)的檢測和組織形態(tài)的觀察可以得到如下結(jié)論：沙海蜇膠原蛋白能夠抑制紫外照射引起的膠原蛋白和水份的流失，其中Mr8000對抑制紫外光照射小鼠皮膚中膠原蛋白的流失最有效而2500Mr8000的膠原肽可以較好的抑制皮膚中水分的流失；沙海蟄膠原肽可以通過提高抗氧化物酶SOD、GSH-Px、CAT的活力起到抗氧化的作用，其中Mr2500的膠原肽對皮膚組織中抗氧化物酶活力的提高起到較好的效果；沙蟄膠原蛋白肽可以有效的改善紫外照射小鼠皮膚的組織形態(tài)，使其接近正常小鼠皮膚的組織形態(tài)；綜上所述，沙海蜇膠原蛋白對光老化小鼠的皮膚具有保護(hù)作用。 3從分子量、劑量、時(shí)間、pH值等幾個(gè)方面沙海蜇膠原蛋白肽的透皮吸收性能進(jìn)行研究。從研究中我們發(fā)現(xiàn)分子量越小的膠原蛋白多肽表現(xiàn)出越好的透皮吸收性能。并且通過累計(jì)透皮吸收量和時(shí)間的曲線得到了沙海蜇膠原蛋白肽體外透皮吸收的零級動力學(xué)方程，， Mr2500: Q=209.29t-6.801;2500Mr8000:Q=84.381t+133.75;Mr8000: Q=52.597t+72.96。隨著樣品濃度的升高，沙海蜇膠原蛋白肽的體外透皮吸收性能也隨之提高。在pH為8左右時(shí)，沙海蜇膠原蛋白多肽表現(xiàn)出較好的體外透皮性能。
[Abstract]:1 the basic composition of salted jellyfish was discussed in this paper. From its basic composition, the water and salt were the main components in pickled jellyfish, and protein was the most important component in dry matter. The protein of collagen accounts for about 1/3, the pickled jellyfish has high water content and high salinity, and the purity of collagen increases the difficulty of extracting collagen. We need to find an effective way to extract collagen from jellyfish. Therefore, the physicochemical properties of three common methods of extracting collagen (enzymatic extraction, hot water extraction and acid extraction) were discussed in this paper. The results showed that the collagen obtained by the three methods was similar in structure. The content of hydroxyproline in collagen extracted by acid method was higher than that of PSC and HEG.The ASC had good emulsifying stability and its molecular weight was relatively large and had good emulsifying property. The order of hygroscopicity from strong to weak is PSCHEGASC.The sample with the best moisture retention is PSC. Considering various factors, hot water was used to extract collagen on a large scale. The collagen was further hydrolyzed and ultrafiltration into peptides with different molecular weight. 2 the protective effect of collagen of jellyfish on the skin of mice skin induced by ultraviolet irradiation was studied. The experimental mice were divided into 11 groups: normal control group, model control group, positive control group, collagen high dose group and collagen low dose group. The results showed that the collagen of jellyfish could inhibit the loss of collagen and water caused by ultraviolet irradiation. Mr8000 is the most effective method to inhibit the loss of collagen in the skin of mice irradiated by ultraviolet light, and the collagen peptide of 2500Mr8000 can effectively inhibit the loss of water in the skin. The collagen peptide of Mr2500 can improve the activity of antioxidant enzyme GSH-PxCAT, and the collagen peptide of Mr2500 can improve the activity of antioxidant enzyme in skin tissue. Jellyfish collagen peptide can effectively improve the skin tissue morphology of ultraviolet irradiated mice, make it close to the normal mouse skin tissue morphology; in summary, jellyfish collagen has a protective effect on the skin of photoaged mice. 3The transdermal absorption of collagen peptide of jellyfish was studied from molecular weight, dose, time and pH value. We found that collagen peptides with lower molecular weight showed better transdermal absorption. The zero-order kinetic equation of collagen peptide transdermal absorption of jellyfish in vitro was obtained by the curve of cumulative transdermal absorption amount and time. Mr 2500: QN 209.29t-6.801 / 2500 Mr 8000: QN 84.381t 133.75m8000: QN 52.597t 72.96. With the increase of sample concentration, the in vitro transdermal absorption of collagen peptide from jellyfish was improved. When pH was about 8, the collagen peptide of jellyfish showed better transdermal properties in vitro.
【學(xué)位授予單位】：中國海洋大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R151

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本文編號：1986131