本文選題：消毒劑 + 滅菌試驗(yàn)��； 參考：《中國消毒學(xué)雜志》2017年01期

【摘要】：目的了解高濃度中和劑及中和產(chǎn)物對消毒劑滅菌試驗(yàn)結(jié)果的影響,為建立專門的消毒劑滅菌試驗(yàn)中和劑選擇試驗(yàn)方法提供依據(jù)。方法以TSB培養(yǎng)基為對照,用2%戊二醛和枯草桿菌黑色變種芽孢進(jìn)行試驗(yàn),觀察含不同中和劑、中和產(chǎn)物的TSB培養(yǎng)基對正常菌和消毒后殘存菌生長的情況。結(jié)果所有配方中和劑及中和產(chǎn)物對正常菌生長無影響,但對殘存菌有抑制作用,尤以含中和產(chǎn)物培養(yǎng)基明顯。結(jié)論消毒劑滅菌試驗(yàn)采用在定量方法鑒定合格的中和劑中加入TSB培養(yǎng)基粉末配制培養(yǎng)基,造成培養(yǎng)體系中和劑及其中和產(chǎn)物濃度過高,可能引起假陰性結(jié)果;消毒后殘存菌的生長復(fù)蘇能力大大弱于正常菌,應(yīng)以消毒后殘存菌進(jìn)行消毒劑滅菌試驗(yàn)中和劑選擇試驗(yàn)。
[Abstract]:Objective to investigate the effect of neutralizing agent and neutralizing product on the result of sterilization test of disinfectant, and to provide the basis for establishing a special method of neutralizing agent selection in sterilizing test of disinfectant. Methods using 2% glutaraldehyde and Bacillus subtilis var. Niger spores as control, the growth of normal and sterilized residual bacteria in TSB medium containing different neutralizing agents and neutralizing products was observed. Results all the neutralizers and neutralization products had no effect on the growth of normal bacteria, but had inhibitory effect on the residual bacteria, especially on the medium containing neutralization products. Conclusion in the sterilization test of disinfectant, adding TSB medium powder into the qualified neutralizer by quantitative method may result in too high concentration of neutralizer and neutralizing product in culture system, which may lead to false negative results. The growth and resuscitation ability of the residual bacteria after disinfection is much weaker than that of the normal bacteria, so the neutralizing agent selection test should be carried out with the residual bacteria after disinfection.
【作者單位】： 四川省疾病預(yù)防控制中心;
【分類號】：R187.1

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本文編號：1951108