本文選題：多環(huán)芳烴 + 外周血淋巴細(xì)胞微核率��； 參考：《華中科技大學(xué)》2013年碩士論文

【摘要】：目的:全面評(píng)價(jià)焦?fàn)t工尿中10種羥基多環(huán)芳烴（monohydroxylated PAHs，OH-PAHs）、血漿二氫二醇環(huán)氧苯并芘（Benzo[a]pyrene-diol-epoxide，BPDE）-白蛋白加合物水平與外周血淋巴細(xì)胞微核率的關(guān)聯(lián)和探索能否找到一個(gè)合適的微核率截?cái)嘀涤糜谠缙诒O(jiān)測(cè)工人的健康狀態(tài)。 方法:選取某焦化廠1315名男性工人作為研究對(duì)象，對(duì)該廠代表性工作場(chǎng)所空氣中多環(huán)芳烴（Polycyclic aromatic hydrocarbons，，PAHs）的濃度進(jìn)行了測(cè)定。分別用氣相色譜-質(zhì)譜聯(lián)用儀和酶聯(lián)免疫吸附實(shí)驗(yàn)測(cè)定工人尿中10種OH-PAHs和血漿BPDE-白蛋白加合物的濃度，用高效液相色譜測(cè)定工人尿中8-羥基鳥苷（8-hydroxy-2’-deoxyguanosine，8-OHdG）的濃度，用胞質(zhì)分裂阻滯法測(cè)定工人外周血淋巴細(xì)胞微核率。 結(jié)果:校正了年齡、工齡、吸煙狀態(tài)、飲酒狀態(tài)的影響后，工人尿1-羥基萘、2-羥基萘、2-羥基芴、9-羥基芴（9-hydroxyfluorene，9-OHFLU）、1-羥基菲、2-羥基菲、3-羥基菲、9-羥基菲（9-hydroxyphenanthrene，9-OHPHE）、1-羥基芘（1-hydroypyrene，1-OHP）、血漿BPDE-白蛋白加合物的水平與外周血淋巴細(xì)胞微核率的關(guān)聯(lián)具有顯著性（P值分別為0.001、0.002、0.001、0.001、0.016、0.001、0.003、0.001、0.001、0.001）。當(dāng)校正了PAHs代謝物間相關(guān)的影響后，工人尿1-OHP、9-OHFLU、血漿BPDE-白蛋白加合物的水平與微核率間的關(guān)聯(lián)依然具有顯著性（經(jīng)過多重比較校正，P值0.0056）。與微核率≤2‰的工人相比，微核率≥5‰的工人尿總OH-PAHs、1-OHP、9-OHFLU、9-OHPHE、8-OHdG和血漿BPDE-白蛋白加合物的水平開始出現(xiàn)顯著增高。趨勢(shì)分析表明在校正了混雜因素的影響后，工人尿總OH-PAHs、1-OHP、9-OHFLU、9-OHPHE、8-OHdG和血漿BPDE-白蛋白加合物的水平從低微核率組到高微核率組呈現(xiàn)顯著升高的趨勢(shì)（P值分別為0.017、0.005、0.002、0.015、0.015、0.001）。 結(jié)論：焦?fàn)t工尿中除了4-羥基菲外的其余9種OH-PAHs和血漿BPDE-白蛋白加合物的水平與外周血淋巴細(xì)胞微核率的關(guān)聯(lián)均具有顯著性。尿1-OHP、9-OHFLU、血漿BPDE-白蛋白加合物與微核率升高間的關(guān)聯(lián)具有顯著性�？梢杂梦⒑寺省�5‰對(duì)焦?fàn)t工進(jìn)行早期健康監(jiān)護(hù)。
[Abstract]:Objective: to evaluate the relationship between the levels of 10 monohydroxylated PAHs OH-PAHs, plasma dihydrodiol epoxide Benzo [a] pyrene-diol-epoxide-albumin adducts and the micronucleus rate of peripheral blood lymphocytes in coke oven workers and to explore whether a suitable micronucleus can be found. The rate truncation value is used to monitor the health status of workers at an early stage. Methods: the concentrations of polycyclic aromatic hydrocarbonsPAHs in the air of typical workplace of a coking plant were determined in 1315 male workers. The concentrations of 10 kinds of OH-PAHs and plasma BPDE- albumin adducts in urine of workers were determined by gas chromatography-mass spectrometry and enzyme-linked immunosorbent assay, respectively. The concentrations of 8-hydroxy-2-hydroxy-deoxyguanosine 8-OHdG in urine of workers were determined by high performance liquid chromatography. The micronucleus rate of peripheral blood lymphocytes of workers was determined by cytokinesis block method. Results: after adjusting the effects of age, length of service, smoking status and drinking state, Urinary 1-hydroxynaphthalene 2-hydroxy-naphthalene 2-hydroxy-2-hydroxyfluorofluorene 9-hydroxyfluorenene-9-OHFLUU 1-hydroxyphenanthroline 2-hydroxy-phenanthroline 3-hydroxyphenanthroline 9-hydroxyphenanthrene9-OHPHEHEN9-hydroxy 1-hydroyrerenenepyrene 1-OHPPPU, the levels of plasma BPDE-albumin adducts were significantly correlated with the micronucleus rate of peripheral blood lymphocytes (P = 0.0010.0020.0020.0010.001P = 0.0010.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001P = 0.001. After adjusting for the correlation between PAHs metabolites, the correlation between the levels of plasma BPDE-albumin adducts and micronucleus rate in the urine of workers with 1-OHP- 9-OHFLU was still significant (P = 0.0056). Compared with workers with micronucleus rate 鈮

本文編號(hào)：1913840