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  本文選題：抗放利 + 輻射防護�。� 參考：《北京協(xié)和醫(yī)學(xué)院》2012年碩士論文

【摘要】：隨著核能和輻射技術(shù)在能源、工農(nóng)業(yè)生產(chǎn)、醫(yī)療衛(wèi)生、科學(xué)研究和國防建設(shè)等領(lǐng)域中的應(yīng)用日益廣泛,許多國家對輻射損傷的預(yù)防和救治都極為重視,對輻射損傷防護藥物的合成、篩選以及作用機制的研究也日益興盛起來。 抗放利,化學(xué)名為2,2-二甲基四氫噻唑鹽酸鹽,是我所研制的具有自主知識產(chǎn)權(quán)的一類新藥,對急性輻射損傷有較好的防護作用。目前國內(nèi)外缺少此化合物的質(zhì)量控制方法,對其輻射防護作用機制的研究較少,本研究采用專屬性較強的氣相色譜法、高效液相色譜法進行了鑒別試驗,采用高效液相色譜法建立了抗放利含量測定的方法,氣相色譜法建立了有機溶劑殘留的檢查方法,AccQ-Tag柱前衍生的方法建立了有關(guān)物質(zhì)的檢查方法,并按照藥典的要求進行了方法學(xué)驗證。 本研究選擇了輻射敏感的造血系統(tǒng)和免疫系統(tǒng)作為研究對象,通過外周血象、MDA含量、SOD活性等指標評價了抗放利對正常小鼠的輻射防護作用,通過小鼠的生存時間、外周血指標、腫瘤生長曲線等指標評價抗放利對荷瘤小鼠的輻射防護作用。實驗結(jié)果提示,抗放利可明顯保護照射引起的免疫器官和造血功能的損傷,使造血功能恢復(fù)顯著加快,增強機體的抗氧化能力,減輕脂質(zhì)過氧化反應(yīng)；抗放利能夠有效地延長受照荷瘤小鼠的生存時間,使造血功能恢復(fù)明顯加快,但不降低腫瘤組織的輻射敏感性。 實驗結(jié)果表明,已建立的研究方法專屬性強、準確性高、精密度好,可以作為抗放利質(zhì)量控制的方法,為進一步制定抗放利質(zhì)量控制標準提供了必要的基礎(chǔ)和依據(jù)；抗放利具有較好的輻射防護作用,能夠選擇性地保護正常組織,其作用機制可能與其保護造血組織、提高機體免疫功能和抗氧化作用有關(guān),該論文的相關(guān)研究為深入研究抗放利的作用機制奠定了基礎(chǔ)。
[Abstract]:With the increasing application of nuclear energy and radiation technology in the fields of energy, industrial and agricultural production, medical and health care, scientific research and national defense construction, many countries attach great importance to the prevention and treatment of radiation damage. Studies on the synthesis, screening and mechanism of radiation protection drugs have become increasingly popular. The chemical name is 2o 2 dimethyl tetrahydrothiazole hydrochloride. It is a kind of new drug with independent intellectual property rights developed by me. It has good protective effect on acute radiation injury. At present, there is a lack of quality control methods for this compound at home and abroad, and the mechanism of radiation protection is less studied. In this study, the specific gas chromatography and high performance liquid chromatography (HPLC) were used to identify the compound. A method for the determination of radioimmunoassay was established by HPLC, and a method for the determination of residual organic solvents was established by gas chromatography. The method for the determination of related substances was established by pre-column derivation of AccQ-Tag. The methodology was validated according to the requirements of pharmacopoeia. In this study, radiation sensitive hematopoietic system and immune system were selected as the research objects. The radiation protective effect of anti-radiation on normal mice was evaluated by the indexes of MDA content and SOD activity in peripheral blood. The survival time and peripheral blood index of mice were also studied. Tumor growth curve was used to evaluate the radiation-protective effect of anti-radiotherapy on tumor-bearing mice. The results suggest that anti-radiation can obviously protect the damage of immune organs and hematopoietic function induced by irradiation, accelerate the recovery of hematopoietic function, enhance the antioxidant ability of the body, and alleviate the lipid peroxidation. Anti-radiotherapy can effectively prolong the survival time of irradiated mice and accelerate the recovery of hematopoietic function, but it does not reduce the radiosensitivity of tumor tissue. The experimental results show that the established research method has strong specificity, high accuracy and good precision. It can be used as a method for quality control of anti-reaping interest, and provides the necessary basis and basis for the further development of quality control standard of anti-reaping interest. The mechanism of anti-radiation protection may be related to the protection of hematopoietic tissue, the enhancement of immune function and the anti-oxidation effect, and the mechanism may be related to the protection of hematopoietic tissue, the enhancement of immune function and the anti-oxidation effect of the body. The related research in this paper lays a foundation for further study on the mechanism of anti-release effect.
【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R142

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本文編號：1884099