本文選題：色氨酸 + 環(huán)磷酰胺�。� 參考：《江南大學(xué)》2012年碩士論文

【摘要】：本文研究了色氨酸對(duì)免疫抑制小鼠和慢性不可預(yù)見(jiàn)性應(yīng)激小鼠氧化應(yīng)激,免疫功能和行為的影響,并對(duì)相應(yīng)指標(biāo)做了相關(guān)性分析,通過(guò)色氨酸對(duì)小鼠氧化應(yīng)激,免疫功能、單胺類(lèi)神經(jīng)遞質(zhì)含量和行為等調(diào)節(jié)作用的研究而進(jìn)一步探討色氨酸的免疫調(diào)節(jié)及抗應(yīng)激作用。 免疫抑制實(shí)驗(yàn)?zāi)Ｐ?60只昆明種雄性小鼠預(yù)飼一周后隨機(jī)分為4組,每組15只:正常對(duì)照組(NC組)、環(huán)磷酰胺免疫抑制組(CTX組)、環(huán)磷酰胺-低劑量色氨酸組(CTX-L組)和環(huán)磷酰胺-高劑量色氨酸組(CTX-H組)。實(shí)驗(yàn)期間腹腔注射環(huán)磷酰胺,灌胃生理鹽水和色氨酸。實(shí)驗(yàn)結(jié)束,收集血樣及組織樣品,中性紅法測(cè)定巨噬細(xì)胞吞噬能力,酶聯(lián)免疫法測(cè)定小鼠IFN-γ和皮質(zhì)醇水平。慢性不可預(yù)見(jiàn)性應(yīng)激實(shí)驗(yàn)?zāi)Ｐ?60只昆明種雄性小鼠預(yù)飼一周后隨機(jī)分為4組,每組15只:正常對(duì)照組(NC組)、慢性不可預(yù)見(jiàn)性應(yīng)激模型組(CUS組)、慢性不可預(yù)見(jiàn)性應(yīng)激-低劑量色氨酸組(CUS-L組)和慢性不可預(yù)見(jiàn)性應(yīng)激-高劑量色氨酸組(CUS-H組)。實(shí)驗(yàn)期間灌胃生理鹽水和色氨酸,同時(shí)給予相應(yīng)的應(yīng)激源刺激。實(shí)驗(yàn)結(jié)束,收集血樣及組織樣品,酶聯(lián)免疫法測(cè)定下丘腦中5-HT和NE含量,水迷宮法測(cè)定小鼠空間學(xué)習(xí)和記憶能力。 實(shí)驗(yàn)結(jié)果:(1)色氨酸可降低應(yīng)激小鼠體內(nèi)活性氧ROS和脂質(zhì)過(guò)氧化物MDA水平(P0.05),提高應(yīng)激小鼠總抗氧化T-AOC能力和超氧化物歧化酶SOD酶活(P0.05)。(2)色氨酸可提高應(yīng)激小鼠的臟器指數(shù),增加腹腔巨噬細(xì)胞吞噬能力和脾淋巴細(xì)胞增殖能力,增加血清中IFN-γ和IL-2水平(P0.05),降低血清IgG和皮質(zhì)醇含量(P0.05)。(3)色氨酸可縮短定位航行實(shí)驗(yàn)應(yīng)激小鼠的逃避潛伏期(P0.05),減少空間搜索實(shí)驗(yàn)小鼠首次到達(dá)原平臺(tái)時(shí)間且增加穿越原平臺(tái)次數(shù)(P0.05)。(4)補(bǔ)充色氨酸可增加應(yīng)激小鼠下丘腦中5-HT含量(P0.05),降低下丘腦NE含量(P0.05),補(bǔ)充色氨酸還可改善應(yīng)激小鼠對(duì)糖水的偏愛(ài)度同時(shí)延長(zhǎng)小鼠冰水游泳時(shí)間(P0.05)。 結(jié)論:環(huán)磷酰胺應(yīng)激小鼠補(bǔ)充色氨酸后可提高機(jī)體抗氧化能力減輕應(yīng)激小鼠的氧化應(yīng)激;免疫抑制小鼠補(bǔ)充色氨酸后可促進(jìn)血清IFN-γ的生成,降低血清皮質(zhì)醇的產(chǎn)生,還可增加小鼠腹腔巨噬細(xì)胞吞噬能力及脾淋巴細(xì)胞的增殖能力,進(jìn)而有效改善由環(huán)磷酰胺應(yīng)激引起的小鼠免疫系統(tǒng)功能的下降;慢性不可預(yù)見(jiàn)性應(yīng)激小鼠補(bǔ)充色氨酸后,通過(guò)增加5-HT水平,降低NE水平而調(diào)節(jié)應(yīng)激小鼠下丘腦中單胺類(lèi)神經(jīng)遞質(zhì)的含量,通過(guò)延長(zhǎng)小鼠冰水游泳時(shí)間而提高應(yīng)激小鼠的抗疲勞能力,給予應(yīng)激小鼠補(bǔ)充色氨酸還可通過(guò)升高血清中細(xì)胞因子IL-2水平,降低血清免疫球蛋白G濃度而調(diào)節(jié)應(yīng)激小鼠的免疫機(jī)能,進(jìn)而對(duì)應(yīng)激小鼠的免疫,空間學(xué)習(xí)記憶力及應(yīng)激行為等進(jìn)行調(diào)節(jié)。
[Abstract]:The effects of tryptophan on oxidative stress, immune function and behavior in immunosuppressive mice and chronic unpredictable stress mice were studied. In order to investigate the immunomodulation and anti-stress effects of tryptophan, the content and behavior of monoamine neurotransmitters were studied. Sixty Kunming male mice were randomly divided into 4 groups after one week of prefeeding. There were 15 rats in each group: normal control group (NC group), cyclophosphamide immunosuppressive group (CTX group), cyclophosphamide low dose tryptophan group (CTX-L group) and cyclophosphamide high dose tryptophan group (CTX-H group). During the experiment, cyclophosphamide was intraperitoneally injected with normal saline and tryptophan. At the end of the experiment, blood samples and tissue samples were collected, phagocytosis of macrophages was measured by neutral red method, and IFN- 緯 and cortisol levels in mice were measured by enzyme-linked immunosorbent assay (Elisa). Sixty Kunming male mice were randomly divided into 4 groups after one week of prefeeding. There were 15 rats in each group: normal control group (NC group), chronic unpredictable stress model group (CUS group), chronic unpredictable stress-low dose tryptophan group (CUS-L group) and chronic unpredictable stress-high dose tryptophan group (CUS-H group). During the experiment, saline and tryptophan were administered intragastrically and stimulated by corresponding stressors. At the end of the experiment, blood samples and tissue samples were collected. The contents of 5-HT and NE in hypothalamus were determined by enzyme-linked immunosorbent assay (Elisa), and the spatial learning and memory abilities of mice were measured by water maze method. The results showed that tryptophan could decrease the levels of reactive oxygen species (ROS) and lipid peroxide (MDA) in stress mice, and increase the total antioxidant T-AOC ability and the activity of superoxide dismutase (SOD). The phagocytosis of peritoneal macrophages and the proliferation of splenic lymphocytes were increased. Increasing the levels of IFN- 緯 and IL-2 in serum and decreasing the contents of serum IgG and cortisol (P0.05) Tryptophan could shorten the escape latency (P0.05), reduce the first time of reaching the original platform and increase the crossing time of mice in space search experiment. Supplementation of tryptophan increased 5-HT content in hypothalamus and decreased NE content in hypothalamus of stress mice. Tryptophan supplementation also improved the preference for sugar water in stress mice and prolonged swimming time of ice water in mice. Conclusion: tryptophan supplementation in cyclophosphamide stress mice can improve the antioxidant capacity and reduce oxidative stress in stressed mice, and tryptophan supplementation in immunosuppressed mice can promote the production of serum IFN- 緯 and decrease the production of serum cortisol. It also increased the phagocytosis of peritoneal macrophages and the proliferation of spleen lymphocytes, and effectively improved the decrease of immune system function induced by cyclophosphamide stress. By increasing the level of 5-HT and decreasing the level of NE, the content of monoamine neurotransmitters in hypothalamus of stress mice was regulated, and the anti-fatigue ability of stress mice was improved by prolonging the swimming time of ice water. Supplementation of tryptophan could also regulate the immune function of stressed mice by increasing the level of cytokine IL-2 in serum and decreasing the concentration of serum immunoglobulin G. Spatial learning memory and stress behavior were regulated.
【學(xué)位授予單位】：江南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R151

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