本文選題：色氨酸 + 環(huán)磷酰胺�。� 參考：《江南大學》2012年碩士論文

【摘要】：本文研究了色氨酸對免疫抑制小鼠和慢性不可預見性應激小鼠氧化應激,免疫功能和行為的影響,并對相應指標做了相關(guān)性分析,通過色氨酸對小鼠氧化應激,免疫功能、單胺類神經(jīng)遞質(zhì)含量和行為等調(diào)節(jié)作用的研究而進一步探討色氨酸的免疫調(diào)節(jié)及抗應激作用。 免疫抑制實驗模型,60只昆明種雄性小鼠預飼一周后隨機分為4組,每組15只:正常對照組(NC組)、環(huán)磷酰胺免疫抑制組(CTX組)、環(huán)磷酰胺-低劑量色氨酸組(CTX-L組)和環(huán)磷酰胺-高劑量色氨酸組(CTX-H組)。實驗期間腹腔注射環(huán)磷酰胺,灌胃生理鹽水和色氨酸。實驗結(jié)束,收集血樣及組織樣品,中性紅法測定巨噬細胞吞噬能力,酶聯(lián)免疫法測定小鼠IFN-γ和皮質(zhì)醇水平。慢性不可預見性應激實驗模型,60只昆明種雄性小鼠預飼一周后隨機分為4組,每組15只:正常對照組(NC組)、慢性不可預見性應激模型組(CUS組)、慢性不可預見性應激-低劑量色氨酸組(CUS-L組)和慢性不可預見性應激-高劑量色氨酸組(CUS-H組)。實驗期間灌胃生理鹽水和色氨酸,同時給予相應的應激源刺激。實驗結(jié)束,收集血樣及組織樣品,酶聯(lián)免疫法測定下丘腦中5-HT和NE含量,水迷宮法測定小鼠空間學習和記憶能力。 實驗結(jié)果:(1)色氨酸可降低應激小鼠體內(nèi)活性氧ROS和脂質(zhì)過氧化物MDA水平(P0.05),提高應激小鼠總抗氧化T-AOC能力和超氧化物歧化酶SOD酶活(P0.05)。(2)色氨酸可提高應激小鼠的臟器指數(shù),增加腹腔巨噬細胞吞噬能力和脾淋巴細胞增殖能力,增加血清中IFN-γ和IL-2水平(P0.05),降低血清IgG和皮質(zhì)醇含量(P0.05)。(3)色氨酸可縮短定位航行實驗應激小鼠的逃避潛伏期(P0.05),減少空間搜索實驗小鼠首次到達原平臺時間且增加穿越原平臺次數(shù)(P0.05)。(4)補充色氨酸可增加應激小鼠下丘腦中5-HT含量(P0.05),降低下丘腦NE含量(P0.05),補充色氨酸還可改善應激小鼠對糖水的偏愛度同時延長小鼠冰水游泳時間(P0.05)。 結(jié)論:環(huán)磷酰胺應激小鼠補充色氨酸后可提高機體抗氧化能力減輕應激小鼠的氧化應激;免疫抑制小鼠補充色氨酸后可促進血清IFN-γ的生成,降低血清皮質(zhì)醇的產(chǎn)生,還可增加小鼠腹腔巨噬細胞吞噬能力及脾淋巴細胞的增殖能力,進而有效改善由環(huán)磷酰胺應激引起的小鼠免疫系統(tǒng)功能的下降;慢性不可預見性應激小鼠補充色氨酸后,通過增加5-HT水平,降低NE水平而調(diào)節(jié)應激小鼠下丘腦中單胺類神經(jīng)遞質(zhì)的含量,通過延長小鼠冰水游泳時間而提高應激小鼠的抗疲勞能力,給予應激小鼠補充色氨酸還可通過升高血清中細胞因子IL-2水平,降低血清免疫球蛋白G濃度而調(diào)節(jié)應激小鼠的免疫機能,進而對應激小鼠的免疫,空間學習記憶力及應激行為等進行調(diào)節(jié)。
[Abstract]:The effects of tryptophan on oxidative stress, immune function and behavior in immunosuppressive mice and chronic unpredictable stress mice were studied. In order to investigate the immunomodulation and anti-stress effects of tryptophan, the content and behavior of monoamine neurotransmitters were studied. Sixty Kunming male mice were randomly divided into 4 groups after one week of prefeeding. There were 15 rats in each group: normal control group (NC group), cyclophosphamide immunosuppressive group (CTX group), cyclophosphamide low dose tryptophan group (CTX-L group) and cyclophosphamide high dose tryptophan group (CTX-H group). During the experiment, cyclophosphamide was intraperitoneally injected with normal saline and tryptophan. At the end of the experiment, blood samples and tissue samples were collected, phagocytosis of macrophages was measured by neutral red method, and IFN- 緯 and cortisol levels in mice were measured by enzyme-linked immunosorbent assay (Elisa). Sixty Kunming male mice were randomly divided into 4 groups after one week of prefeeding. There were 15 rats in each group: normal control group (NC group), chronic unpredictable stress model group (CUS group), chronic unpredictable stress-low dose tryptophan group (CUS-L group) and chronic unpredictable stress-high dose tryptophan group (CUS-H group). During the experiment, saline and tryptophan were administered intragastrically and stimulated by corresponding stressors. At the end of the experiment, blood samples and tissue samples were collected. The contents of 5-HT and NE in hypothalamus were determined by enzyme-linked immunosorbent assay (Elisa), and the spatial learning and memory abilities of mice were measured by water maze method. The results showed that tryptophan could decrease the levels of reactive oxygen species (ROS) and lipid peroxide (MDA) in stress mice, and increase the total antioxidant T-AOC ability and the activity of superoxide dismutase (SOD). The phagocytosis of peritoneal macrophages and the proliferation of splenic lymphocytes were increased. Increasing the levels of IFN- 緯 and IL-2 in serum and decreasing the contents of serum IgG and cortisol (P0.05) Tryptophan could shorten the escape latency (P0.05), reduce the first time of reaching the original platform and increase the crossing time of mice in space search experiment. Supplementation of tryptophan increased 5-HT content in hypothalamus and decreased NE content in hypothalamus of stress mice. Tryptophan supplementation also improved the preference for sugar water in stress mice and prolonged swimming time of ice water in mice. Conclusion: tryptophan supplementation in cyclophosphamide stress mice can improve the antioxidant capacity and reduce oxidative stress in stressed mice, and tryptophan supplementation in immunosuppressed mice can promote the production of serum IFN- 緯 and decrease the production of serum cortisol. It also increased the phagocytosis of peritoneal macrophages and the proliferation of spleen lymphocytes, and effectively improved the decrease of immune system function induced by cyclophosphamide stress. By increasing the level of 5-HT and decreasing the level of NE, the content of monoamine neurotransmitters in hypothalamus of stress mice was regulated, and the anti-fatigue ability of stress mice was improved by prolonging the swimming time of ice water. Supplementation of tryptophan could also regulate the immune function of stressed mice by increasing the level of cytokine IL-2 in serum and decreasing the concentration of serum immunoglobulin G. Spatial learning memory and stress behavior were regulated.
【學位授予單位】：江南大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R151

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