本文選題：飲用水 + 有機提取物��； 參考：《環(huán)境與職業(yè)醫(yī)學》2015年03期

【摘要】：[目的]觀察飲用水有機提取物對大鼠肝臟谷胱甘肽-S-轉(zhuǎn)移酶(glutathione-S-transferase,GSTs)活性及谷胱甘肽-S-轉(zhuǎn)移酶A1(GSTA1)基因m RNA和蛋白表達的影響,探討其在飲用水有機提取物肝臟損傷中作用。[方法]采用固相萃取法提取水樣中有機污染物,50只SD大鼠隨機分成5組,分別為空白對照組、溶劑對照組(玉米油)和低、中、高3個染毒組(劑量分別為每天5、20、80 L/kg·bw),進行經(jīng)口灌胃染毒12周。分光光度法檢測GSTs的活性,實時熒光定量聚合酶鏈反應法和Western blot法分別檢測GSTA1基因的m RNA和蛋白質(zhì)表達水平,同時檢測肝功能各指標。[結(jié)果](1)大鼠肝臟GSTs酶活性:與空白對照、溶劑對照及低劑量組相比,中劑量[(50.66±5.62)U/mg蛋白]和高劑量組[(39.80±12.95)U/mg蛋白]的GSTs酶活性升高(P0.05);與中劑量組相比,高劑量組GSTs的酶活性則明顯降低(P0.05)。(2)GSTA1的m RNA及蛋白表達水平:中、高劑量組高于空白對照組、溶劑對照及低劑量組(P0.05);而與中劑量組相比,高劑量組GSTA1的m RNA表達水平下降(P0.05)。Western blot檢測結(jié)果顯示,隨染毒劑量的增加,GSTA1蛋白表達呈先升高后降低的趨勢,與空白對照、溶劑對照及低劑量組比較,中、高劑量組的升高,差異具有統(tǒng)計學意義(P0.05);而與中劑量組比較,高劑量GSTA1的蛋白表達則下降(P0.05)。(3)肝功能指標:與對照組比較,血清膽堿酯酶(CHE)在中、高劑量染毒組升高,差異均具有統(tǒng)計學意義(P0.05);丙氨酸氨基轉(zhuǎn)移酶(ALT)、天冬氨酸氨基轉(zhuǎn)換酶(AST)則僅在高劑量組升高(P0.05)�？偟鞍�(TP)和白蛋白(ALB)在高劑量組下降(P0.05)。GSTA1蛋白表達、GSTs活性與染毒大鼠肝臟CHE水平均呈正相關關系(r=0.490 5,r=0.685 2;P0.05)。[結(jié)論]在本實驗條件下,飲用水有機提取物較高劑量染毒可上調(diào)大鼠肝臟GSTA1的m RNA及蛋白質(zhì)表達水平,調(diào)控GSTs活性改變,從而導致大鼠肝細胞對毒物的易感性增強,肝損傷加重。
[Abstract]:[Objective] to observe the effects of organic extracts from drinking water on the activity of glutathione -S- transferase (glutathione-S-transferase, GSTs) and the expression of M RNA and protein of glutathione -S- transferase A1 (GSTA1) gene in rat liver, and to explore the use of solid phase extraction in the extraction of organic pollutants in water samples by the method of solid phase extraction. 50 SD rats were randomly divided into 5 groups: blank control group, solvent control group (corn oil) and low, middle and high 3 infected groups (5,20,80 L/kg / BW per day respectively) for 12 weeks by oral gavage. The activity of GSTs was detected by spectrophotometric method, GSTA1 base was detected by real-time quantitative polymerase chain reaction and Western blot method, respectively. The expression level of M RNA and protein, and the detection of liver function. [results] (1) the activity of GSTs enzyme in the liver of rats: compared with the blank control, the medium dose [(50.66 + 5.62) U/mg protein] and high dose group [(39.80 + 12.95) U/mg protein] increased GSTs activity compared with the blank control group (P0.05), and the high dose group GSTs compared with the medium dose group. The activity of the enzyme decreased significantly (P0.05). (2) the m RNA and protein expression level of GSTA1 were higher in the high dose group than in the blank control group, the solvent control and the low dose group (P0.05), and the m RNA expression level of GSTA1 in the high dose group decreased (P0.05).Western blot detection results showed that the expression of the GSTA1 protein expressed with the increase of the dose. The trend of first increasing and then decreasing, compared with the blank control, compared with the solvent control and low dose group, the high dose group increased, the difference was statistically significant (P0.05), but compared with the middle dose group, the protein expression of high dose GSTA1 decreased (P0.05). (3) the liver function index: compared with the control group, the serum cholinesterase (CHE) was in the high dose group. The difference was statistically significant (P0.05); alanine aminotransferase (ALT), aspartic aminotransferase (AST) increased only in high dose group (P0.05). Total protein (TP) and albumin (ALB) decreased (P0.05).GSTA1 protein expression in high dose group, GSTs activity was positively correlated with CHE level of rat liver (r=0.490 5, r=0.685 2). 0.05) [Conclusion] [Conclusion] in this experiment, the high dose of organic extracts from drinking water can increase the level of M RNA and protein expression of rat liver GSTA1, regulate the changes of GSTs activity, and lead to the enhancement of the susceptibility to poison in rat liver cells and the aggravation of liver injury.

【作者單位】： 貴陽醫(yī)學院公共衛(wèi)生學院;
【基金】：貴州省科技廳資助項目(編號:黔科合J字[2012]2041號) 貴陽市科學技術計劃項目(編號:筑科合同[2013103]19號)
【分類號】：R114

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本文編號：1871525