本文選題：綠原酸 + 腸道屏障功能�。� 參考：《南昌大學(xué)》2013年碩士論文

【摘要】：本實(shí)驗(yàn)旨在探討綠原酸對(duì)斷奶大鼠免疫功能和腸道屏障功能的影響。選用32只健康的21±1日齡斷奶SD大鼠(62.26±2.73g),按體重隨機(jī)分成4個(gè)組：正常組,LPS組(第13天1mg/Kg.BW LPS),CHA20組(每天20mg/kg CHA),CHA50組(每天50mg/kg CHA),每組8只大鼠,試驗(yàn)期為14天。本實(shí)驗(yàn)首先研究綠原酸對(duì)斷奶大鼠不同時(shí)間段內(nèi)生長(zhǎng)性能的影響,隨后對(duì)斷奶大鼠在13天注射脂多糖加強(qiáng)應(yīng)激,研究綠原酸干預(yù)對(duì)斷奶大鼠脂多糖應(yīng)激生理功能、免疫功能以及腸道屏障功能的影響。 實(shí)驗(yàn)結(jié)果表明,CHA50組對(duì)斷奶大鼠生長(zhǎng)性能有較好的的促進(jìn)作用,能顯著降低7-14d料肉比(p0.05)；脂多糖應(yīng)激斷奶大鼠后導(dǎo)致小腸長(zhǎng)度指數(shù)、脾重指數(shù)、血清IgM水平、空腸和回腸絨毛高度和絨腺比、結(jié)腸和空腸Occludin和ZO-1蛋白表達(dá)、結(jié)腸和空腸組織IL-10水平顯著降低(p0.05)以及顯著增加(p0.05)血清IL-2和IFN-γ水平、IFN-γ/IL-4比值、血清尿素氮和白蛋白濃度、血清DAO濃度、空腸和回腸隱窩深度、尿液中L/M比值、結(jié)腸和空腸組織中IFN-γ和TNF-α的含量。綠原酸能顯著增加(p0.05)小腸長(zhǎng)度指數(shù)、脾重指數(shù)、血清IgM含量、空腸和回腸絨毛高度和絨腺比、結(jié)腸和空腸Occludin和ZO-1蛋白表達(dá)、空腸組織IL-10水平；顯著降低(p0.05)血清IL-2和IFN-γ水平、IFN-γ/IL-4比值、尿素氮和谷丙轉(zhuǎn)氨酶濃度、空腸和回腸隱窩深度、尿液中L/M比值、血清DAO濃度、結(jié)腸和空腸組織中IFN-γ和TNF-α的含量。 綜上所述,綠原酸能促進(jìn)斷奶大鼠的生長(zhǎng)發(fā)育,緩解斷奶大鼠造成的免疫力降低、腸道形態(tài)結(jié)構(gòu)和屏障功能損傷,降低腸道通透性,調(diào)節(jié)腸道的炎癥反應(yīng),提高機(jī)體的免疫功能以及腸道屏障功能。
[Abstract]:The aim of this experiment was to investigate the effects of chlorogenic acid on the immune function and intestinal barrier function of weaned rats. 32 healthy SD rats (62.26 + 2.73g) were randomly divided into 4 groups according to weight: normal group, LPS group (thirteenth days 1mg/Kg.BW LPS), CHA20 group (20mg/ kg CHA every day), CHA50 group (50mg/kg CHA), 8 rats in each group. The period was 14 days. The effect of chlorogenic acid on the growth performance of rats in different periods of weaning was first studied. Then the rats were injected with lipopolysaccharide for 13 days to strengthen stress, and the effects of chlorogenic acid on the physiological function, immune function and intestinal barrier function of LPS in weaned rats were studied.
The experimental results showed that the CHA50 group had a better promoting effect on the growth performance of the weaned rats and significantly reduced the ratio of 7-14d to meat (P0.05). After the lipopolysaccharide stressed weaning rats, the length index of the small intestine, the spleen weight index, the serum IgM level, the height of the jejunum and ileum villus and the ratio of the cashmere gland, the expression of Occludin and ZO-1 in the colon and jejunum, and the colon and the colon were observed. IL-10 levels in the jejunum tissue were significantly reduced (P0.05) and significantly increased (P0.05) serum IL-2 and IFN- gamma levels, IFN- gamma /IL-4 ratio, serum urea nitrogen and albumin concentration, serum DAO concentration, jejunum and ileum depth, urine L/M ratio, the content of IFN- gamma and TNF- alpha in the colon and jejunum tissue. Chlorogenic acid could significantly increase the length of the small intestine (P0.05) small intestine length Degree index, spleen weight index, serum IgM content, jejunum and ileum villus height and cashmere gland ratio, Occludin and ZO-1 protein expression in colon and jejunum, IL-10 level in jejunum, serum IL-2 and IFN- gamma level, IFN- gamma /IL-4 ratio, urea nitrogen and glutamic transutase concentration, jejunum and ileum depth, L/M ratio in urine, L/M ratio in urine, D serum, serum D. AO concentration, IFN- and TNF- alpha in colon and jejunal tissues.
To sum up, chlorogenic acid can promote the growth and development of weaned rats, alleviate the decrease of immunity caused by weaning rats, the damage of intestinal morphology and barrier function, reduce the intestinal permeability, regulate the intestinal inflammatory reaction, improve the immune function of the body and the intestinal barrier function.

【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R151.2

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