本文選題：圍產(chǎn)期 + 壬基酚　； 參考：《環(huán)境與健康雜志》2017年04期

【摘要】：目的探討圍產(chǎn)期壬基酚(nonylphenol,NP)暴露導(dǎo)致成年雄性仔鼠糖脂代謝紊亂及其機(jī)制。方法將32只健康成年SPF級(jí)SD妊娠大鼠隨機(jī)分為普通飼料對(duì)照(玉米油)組、普通飼料+NP(200 mg/kg)組、高脂高糖飼料(15%豬油、20%白糖、65%普通飼料)對(duì)照組、高脂高糖飼料+NP(200 mg/kg)組,每組8只。母鼠從妊娠第6天至產(chǎn)后21 d(PND21,斷乳)清晨空腹灌胃染毒NP,染毒容量為5 ml/kg,每天1次。每組隨機(jī)選取10只雄性仔鼠,于出生70 d后檢測(cè)胰腺組織中NP、血糖及血清中甘油三酯(triglycerides,TG)、總膽固醇(total cholesterol,TC)的水平,并檢測(cè)胰腺糖代謝相關(guān)基因葡萄糖激酶(glucokinase,GCK)和葡萄糖轉(zhuǎn)運(yùn)蛋白-2(glucose transporter-2,GLUT-2)及脂肪細(xì)胞分化關(guān)鍵基因過(guò)氧化物酶體增殖物激活受體γ(peroxisome proliferator activated receptorγ,PPARγ)和脂肪酸合成酶(fatty acid sythase,FAS)的表達(dá)水平。結(jié)果與普通飼料對(duì)照組比較,第4、6周高脂高糖飼料對(duì)照組和高脂高糖飼料+NP組仔鼠的體重均大于普通飼料對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。第8周時(shí),高脂高糖飼料+NP組仔鼠高脂高糖飼料對(duì)照組普通飼料對(duì)照組(P0.05),且高脂高糖飼料+NP組仔鼠高脂高糖飼料對(duì)照組普通飼料+NP組(P0.05)。與普通飼料對(duì)照組比較,普通飼料+NP組及高脂高糖飼料+NP組仔鼠的胰腺NP、血糖及血清TG、TC水平均增高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。高脂高糖飼料+NP組仔鼠的胰腺NP、血糖及血清TG、TC水平均高于普通飼料+NP組(P0.05)。各組仔鼠胰腺GCK、GLUT-2基因的表達(dá)水平依次為高脂高糖飼料+NP組普通飼料+NP組、高脂高糖飼料對(duì)照組普通飼料對(duì)照組,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。各組仔鼠脂肪細(xì)胞PPARγ、FAS基因的表達(dá)水平依次為高脂高糖飼料+NP組普通飼料+NP組高脂高糖飼料對(duì)照組普通飼料對(duì)照組,除普通飼料+NP組與高脂高糖飼料對(duì)照組比較的FAS基因外,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論圍產(chǎn)期NP暴露可導(dǎo)致仔鼠糖脂代謝紊亂,其機(jī)制可能與NP促進(jìn)胰腺糖代謝和脂肪細(xì)胞分化關(guān)鍵基因表達(dá)有關(guān)。
[Abstract]:Objective to investigate the metabolic disorder and mechanism of glycolipid metabolism in adult male offspring rats by perinatal nonylphenol (NP) exposure. Methods 32 healthy adult SPF grade SD pregnant rats were randomly divided into normal feed control (corn oil) group, ordinary feed +NP (200 mg/kg) group, high fat and high sugar diet (15% lard, 20% white sugar, 65% ordinary feed) control group, high fat diet. High glucose diet +NP (200 mg/kg) group, 8 mice in each group. From sixth days of pregnancy to 21 d postpartum (PND21, weaning), NP was administered to the stomach in the morning, the volume was 5 ml/kg, 1 times a day. 10 male offspring were randomly selected from each group. After the birth of 70 D, the blood glucose and serum triglyceride (triglycerides, TG), and total cholesterol (total Chol) were detected. The levels of esterol, TC) and the detection of glucokinase (glucokinase, GCK) and glucose transporter -2 (glucose transporter-2, GLUT-2) and the key genes for the differentiation of lipid cells and the peroxisome proliferator activated receptor gamma (peroxisome proliferator activated receptor gamma) and fatty acid synthetase. The expression level of acid sythase, FAS). Compared with the normal diet control group, the weight of the high fat and high sugar diet control group and the high fat and high sugar feed +NP group was higher than the normal diet control group at week 4,6, and the difference was statistically significant (P0.05). At the eighth week, the high fat high sugar diet + NP group of high fat and high sugar diet control group was compared with the normal diet control group Group (P0.05), and high fat and high sugar diet +NP group of high fat and high sugar diet control group +NP group (P0.05). Compared with the normal diet control group, the pancreas NP, blood sugar and serum TG, TC level in the +NP group and the high fat and high sugar diet +NP group were all higher, the difference was statistically significant (P0.05). P, blood glucose and serum TG, TC level were higher than that of normal diet +NP group (P0.05). The expression level of GLUT-2 gene in each group was in order of the +NP group of high fat and high sugar feed +NP common feed, and the control group of high fat and high sugar diet control group, the difference was statistically significant (P0.05). The expression of PPAR gamma, FAS gene expression in each group of adipocytes in each group The level of high fat and high sugar diet +NP group +NP high fat and high sugar diet control group common feed control group, except the common feed +NP group and high fat diet control group FAS gene, the difference was statistically significant (P0.05). Conclusion perinatal exposure to NP can lead to lipid metabolism disorder in offspring rats, the mechanism may be promoted with NP Glycometabolism and adipocyte differentiation are related to the expression of key genes in pancreas.

【作者單位】： 遵義醫(yī)學(xué)院公共衛(wèi)生學(xué)院;遵義醫(yī)學(xué)院附屬醫(yī)院內(nèi)分泌科;遵義醫(yī)學(xué)院附屬醫(yī)院胃腸外科;
【基金】：國(guó)家自然科學(xué)基金(81360439;81560527) 貴州省優(yōu)秀青年人才基金(2017) 貴州省教育廳青年基金(黔教合KY字[2013]198) 貴州省科技廳遵義醫(yī)學(xué)院聯(lián)合基金重點(diǎn)項(xiàng)目(黔科合LH字[2014]7543;[2015]7521) 遵義醫(yī)學(xué)院重點(diǎn)學(xué)科建設(shè)經(jīng)費(fèi);遵義醫(yī)學(xué)院招標(biāo)課題(2013F-68)
【分類號(hào)】：R114

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