發(fā)布時(shí)間：2018-05-09 05:12

  本文選題：-丁二烯 + 彗星試驗(yàn)��； 參考：《衛(wèi)生研究》2015年02期

【摘要】：目的探討1,3-丁二烯(BD)作業(yè)工人外周血淋巴細(xì)胞遺傳物質(zhì)損傷狀況及其與細(xì)胞周期蛋白D1(CCND1)基因多態(tài)性的關(guān)系。方法采用外周血淋巴細(xì)胞胞質(zhì)分裂阻滯微核(CBMN)試驗(yàn)和彗星試驗(yàn)評(píng)價(jià)171名BD作業(yè)工人和55名非職業(yè)暴露對(duì)照人群的染色體和DNA損傷水平,應(yīng)用聚合酶鏈反應(yīng)-限制性片段長度多態(tài)性方法分析CCND1基因的多態(tài)性。同時(shí),通過問卷調(diào)查的方式了解被調(diào)查者的年齡、性別、吸煙、飲酒和個(gè)人職業(yè)暴露史。結(jié)果 BD作業(yè)工人外周血淋巴細(xì)胞微核率和彗星尾距分別為(7.17±5.09)‰和4.65(95%CI 3.49~5.98),均高于對(duì)照組(3.82±2.37)‰和2.38(95%CI 0.82~3.98),差異有統(tǒng)計(jì)學(xué)意義(P0.01);暴露組中具有CCND1基因A870G位點(diǎn)GG基因型個(gè)體的彗星尾距顯著低于AA基因型個(gè)體(P0.05)。結(jié)論在現(xiàn)有BD暴露水平下,外周血淋巴細(xì)胞胞質(zhì)分裂阻滯微核法和彗星試驗(yàn)均能檢出BD作業(yè)工人的遺傳物質(zhì)損傷;CCND1等位基因A870G位點(diǎn)GG基因型可能與職業(yè)性BD暴露導(dǎo)致的外周血淋巴細(xì)胞DNA損傷水平增加風(fēng)險(xiǎn)相關(guān)聯(lián)。
[Abstract]:Objective to investigate the genetic material damage in peripheral blood lymphocytes of workers exposed to 1-butadiene-butadiene (BDB) and its association with the polymorphism of cyclin D _ (1) C _ (CND _ 1) gene. Methods the chromosomal and DNA damage levels in 171 BD exposed workers and 55 non-occupational exposed controls were evaluated by cytoplasmic mitotic block micronucleus (DNA) assay and comet assay in peripheral blood lymphocytes. Polymerase chain reaction-restriction fragment length polymorphism (RFLP) was used to analyze the polymorphism of CCND1 gene. At the same time, the age, sex, smoking, drinking and personal occupational exposure were investigated by questionnaire. Results the micronucleus rate and comet tail distance of peripheral blood lymphocytes in BD exposed workers were 7.17 鹵5.09 鈥，

本文編號(hào)：1864722