發(fā)布時間：2018-05-05 05:06

  本文選題：甲醛 + 胚胎��； 參考：《延邊大學(xué)》2014年碩士論文

【摘要】：目的：本實驗旨在研究甲醛對孕鼠胚胎心臟發(fā)育的毒性作用。 .方法：選擇成功受孕的成年健康SD大鼠隨機(jī)分四組,每組5只分別吸入不同濃度甲醛：第1組(對照組)：吸入新鮮空氣,甲醛濃度接近于0mg/m3、第2組(低濃度組)：(4.8±1.3)mg/m3、第3組(中濃度組)：(25.24±4.6)mg/m3、第4組(高濃度組)：(119.64±15.4)mg/m3。各組大鼠均從孕第7天開始每日上午9：00吸入不同濃度甲醛進(jìn)行染毒,每日2小時,連續(xù)染毒12天,建立染毒模型。待各組孕鼠自然分娩后,進(jìn)行新生小鼠胚胎發(fā)育的一般毒性、心臟毒性檢測,同時觀察心臟的病理學(xué)改變。 結(jié)果： 1、新生小鼠胚胎發(fā)育的一般毒性：四組新生小鼠平均出生體重分別為7.96g、7.88g、7.80g、7.01g。高濃度組分別與其它組兩兩比較,P值均0.05。 2、新生小鼠心臟發(fā)育的毒性：四組新生小鼠發(fā)生心臟畸形率分別為：0%、0%、0%、8.9%。高濃度組致小鼠畸形率明顯高于其它組。 3、血清天門冬氨酸氨基轉(zhuǎn)移酶水平：四組新生小鼠平均AST值分別為162.1U/L、170.0UL、207.3U/L和318.7U/L。各組間兩兩相比較,高濃度組、中濃度組小鼠血清中平均天門冬氨酸氨基轉(zhuǎn)移酶水平明顯高于低濃度組及正常對照組。 4、心臟組織超微結(jié)構(gòu)改變：正常對照組心肌組織電鏡下超微結(jié)構(gòu)基本正常,高濃度組可見心肌組織肌原纖維排列紊亂；線粒體腫大,大小不一,嵴斷裂、溶解,有較多空泡形成；線粒體間糖原顆粒少。 5、心臟組織HE染色：正常對照組心肌細(xì)胞正常,可見橫紋,無變性壞死；甲醛低濃度組和中濃度組與正常組類似,但可見少量淋巴細(xì)胞侵潤；甲醛高濃度組心臟有明顯水腫變性,細(xì)胞胞漿內(nèi)有顆粒樣物質(zhì),個別細(xì)胞可見核濃縮、核碎裂等細(xì)胞壞死表現(xiàn),心肌細(xì)胞橫紋不清。 結(jié)論1、甲醛具有胚胎毒性,可降低新生小鼠出生體重。 2、甲醛可導(dǎo)致新生小鼠心肌細(xì)胞發(fā)生變性壞死,影響心肌細(xì)胞正常功能。 3、甲醛對小鼠胚胎心臟可產(chǎn)生毒性作用。
[Abstract]:Objective: to study the toxic effect of formaldehyde on embryonic heart development in pregnant rats. . Methods: healthy adult SD rats who were successfully conceived were randomly divided into four groups with 5 rats in each group inhaling different concentrations of formaldehyde: group 1 (control group: inhalation of fresh air) The concentration of formaldehyde was close to 0 mg / m 3, group 2 (low concentration group: 4.8 鹵1.3 mg / m 3), group 3 (middle concentration group: 25.24 鹵4.6 mg / m 3), group 4 (high concentration group: 119.64 鹵15.4mg / m 3). The rats in each group were exposed to different concentrations of formaldehyde at 9:00 every day from the 7th day of pregnancy for 2 hours daily for 12 consecutive days to establish the model of exposure. The common toxicity and cardiac toxicity of embryonic development of newborn mice were detected after the natural delivery of pregnant mice in each group, and the pathological changes of heart were observed at the same time. Results: 1. The general toxicity of embryonic development in newborn mice: the average birth weight of newborn mice in the four groups was 7.96g / 7.88g / 7.80g / 7.01grespectively. Compared with other groups, the P value of high concentration group was 0.05. 2. The toxicity of cardiac development in newborn mice: the incidence of cardiac malformation in the four groups of newborn mice was respectively 1: 0 and 8. 9%. The deformity rate of mice in high concentration group was significantly higher than that in other groups. 3, serum aspartate aminotransferase level: the average AST values of the four groups of newborn mice were 162.1 U / L 170.0ULN 207.3 U / L and 318.7 U / L, respectively. The average aspartate aminotransferase level in the serum of the mice in the high concentration group and the middle concentration group was significantly higher than that in the low concentration group and the normal control group. (4) the ultrastructural changes of cardiac tissue: the ultrastructure of myocardial tissue in the normal control group was basically normal under electron microscope, and in the high concentration group, the myofibril arrangement of myocardial tissue was disordered, the mitochondria was enlarged and varied in size, the crest was broken and dissolved, There were more vacuoles and less glycogen granules between mitochondria. 5, HE staining of heart tissue: normal control group showed normal myocardial cells with striation and no degeneration and necrosis, low and middle concentration groups of formaldehyde were similar to normal group, but a small amount of lymphocytes were infiltrated. In the high concentration group of formaldehyde, there were obvious edema and degeneration in the heart, granule like substance in the cytoplasm of the cells, nuclear concentration, nuclear fragmentation and other cell necrosis manifestations in individual cells, and the cross lines of the cardiac myocytes were unclear. Conclusion 1. Formaldehyde has embryotoxicity and can reduce birth weight of newborn mice. 2, formaldehyde can cause degeneration and necrosis of neonatal mouse cardiomyocytes and affect the normal function of cardiomyocytes. 3, formaldehyde can produce toxic effect on mouse embryonic heart.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R114

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本文編號：1846319