本文選題：流式細胞技術(shù)(FCM) + 低核酸含量(LNA)細菌　； 參考：《微生物學通報》2016年09期

【摘要】：【目的】增加低核酸含量(LNA)細菌與過濾性細菌之間的認識�！痉椒ā坎捎昧魇郊毎夹g(shù)(FCM)、變性梯度凝膠電泳(DGGE)及統(tǒng)計學分析研究3種典型淡水環(huán)境中細菌群落與濾過性�！窘Y(jié)果】LNA細菌與過濾性細菌在數(shù)量上具有很好的相關(guān)性(y=0.646x+22.42,R2=0.984,P0.01),細菌的濾過性不僅與細菌大小有關(guān),還與細胞整體形狀及其伸縮性有關(guān);細菌群落組織與LNA細菌比重呈負相關(guān)性,而與HNA細菌呈正相關(guān)性�！窘Y(jié)論】0.45μm膜過濾對水樣微生物群落中的LNA細菌具有極強的篩選效果;細菌群落組織與其基于流式細胞技術(shù)測定的基因含量具有密切聯(lián)系。
[Abstract]:[objective] to increase the understanding between low nucleic acid content (LNA) bacteria and filter bacteria. [methods] flow cytometry, denaturing gradient gel electrophoresis (DGGE) and statistical analysis were used to study the relationship between bacterial communities and bacteria in three typical freshwater environments. [results] there was a good correlation between the number of LNA bacteria and the number of filter bacteria. The filterability of LNA bacteria was not only related to the size of bacteria, but also to the number of bacteria. There was a negative correlation between bacterial community and the specific gravity of LNA bacteria, but a positive correlation with HNA bacteria. [conclusion] 0.45 渭 m membrane filtration has a strong screening effect on LNA bacteria in water sample microbial community. Bacterial community tissue is closely related to the gene content determined by flow cytometry.
【作者單位】： 南開大學環(huán)境科學與工程學院環(huán)境污染過程與基準教育部重點實驗室天津市城市生態(tài)環(huán)境修復與污染防治重點實驗室;
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本文編號：1839029