發(fā)布時間：2018-04-30 03:12

  本文選題：手持式分析儀 + 智能手機��； 參考：《傳感技術(shù)學報》2017年12期

【摘要】：石房蛤毒素(STX)是常見的麻痹性貝毒(PSP),通過食物鏈積累在貝類食品中,人類若食用這種有毒的貝類會影響健康,甚至中毒。因此迫切需要一種簡便的方法來檢測這種麻痹性貝毒以避免中毒事件的發(fā)生�；诟偁幮悦庖咴淼脑嚰垪l可以和石房蛤毒素產(chǎn)生特異性顯色反應,采用研發(fā)的新型手持式分析儀進行檢測分析。毒素分析儀采用智能手機作為光探測器,配合3D打印配件,再通過智能手機上研發(fā)的用于圖像采集和數(shù)據(jù)處理的應用程序,可對顯色后的試紙條進行分析處理并得出所測毒素的濃度。這種方法簡單、快速、檢出限低、靈敏度高。目前,本儀器可測得的STX的檢出限:5.2 ng/m L,檢測范圍:5.2 ng/m L~100 ng/m L。實驗表明,本文研制的基于智能手機系統(tǒng)和免疫分析的手持式試紙條分析儀有望成為對麻痹性貝毒進行現(xiàn)場快速檢測的有效工具。
[Abstract]:STX is a common paralytic shellfish poison, which accumulates in shellfish food through the food chain. If humans eat this poisonous shellfish, it will affect their health and even be poisoned. Therefore, a simple method is urgently needed to detect the paralytic shellfish poison to avoid poisoning. The test strip based on the principle of competitive immunity can produce specific color reaction with clam toxin, and a new hand-held analyzer is developed for detection and analysis. The toxin analyzer uses a smart phone as a photodetector, along with 3D printing accessories, and then uses an application developed on the smartphone for image acquisition and data processing. The color-developing test strip can be analyzed and treated and the concentration of the toxin can be obtained. This method is simple, rapid, low detection limit and high sensitivity. At present, the detection limit of STX measured by this instrument is 1: 5.2 ng/m / L, and the detection range is 1: 5.2 ng/m / L ~ (100 ng/m / L). The experimental results show that the hand-held test strip analyzer based on smart phone system and immune analysis is expected to be an effective tool for rapid detection of paralytic shellfish virus in the field.
【作者單位】： 浙江大學生物傳感器國家專業(yè)實驗室生物醫(yī)學工程教育部重點實驗室生儀學院;中國科學院傳感技術(shù)國家重點實驗室;
【基金】：國家海洋公益專項項目(201305010)
【分類號】：R155.3;TH83

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