本文選題：自來(lái)水 + 非苛養(yǎng)細(xì)菌　； 參考：《軍事醫(yī)學(xué)》2017年08期

【摘要】：目的調(diào)查天津某地小區(qū)生活飲用水中非苛養(yǎng)耐藥菌的污染狀況。方法采用R2A瓊脂對(duì)自來(lái)水進(jìn)行細(xì)菌分離,利用16S rRNA基因測(cè)序法對(duì)細(xì)菌分離株進(jìn)行種屬鑒定。同時(shí)采用Kirby-Bauer紙片擴(kuò)散法(K-B紙片法)進(jìn)行細(xì)菌的抗生素敏感性試驗(yàn)。結(jié)果在分離的39株非苛養(yǎng)菌中,耐藥菌占79.49%,耐藥細(xì)菌涉及腸球菌屬、葡萄球菌屬、不動(dòng)桿菌屬、假單胞菌屬。其中,僅對(duì)一種抗生素耐藥的細(xì)菌占28.21%,而多藥耐藥菌則占51.28%。耐藥菌對(duì)復(fù)方新諾明的耐藥最為普遍,耐藥率為53.85%;對(duì)磺胺甲VA唑的耐藥次之,占28.21%。結(jié)論該小區(qū)生活飲用水中的非苛養(yǎng)耐藥菌污染較為嚴(yán)重,應(yīng)加強(qiáng)關(guān)注其潛在的人群風(fēng)險(xiǎn)。
[Abstract]:Objective to investigate the contamination of non-resistant bacteria in drinking water in Tianjin. Methods R2A Agar was used to isolate bacteria from tap water and 16s rRNA gene sequencing method was used to identify the species of bacteria isolated from tap water. At the same time, Kirby-Bauer disk diffusion method and K B disk method were used to test the antibiotic sensitivity of bacteria. Results among the 39 strains of non-caustic culture bacteria, resistant bacteria accounted for 79.49. The resistant bacteria involved Enterococcus, Staphylococcus, Acinetobacter, Pseudomonas. Among them, only one antibiotic resistant bacteria accounted for 28. 21%, and multi-drug resistant bacteria accounted for 51. 28%. The resistance of drug-resistant bacteria to compound sulfamethoxazole was the most common, and the resistance rate was 53.85, followed by sulfamethazol (28.21%). Conclusion the contamination of non-resistant bacteria in drinking water in this district is serious, so we should pay more attention to the potential population risk.
【作者單位】： 軍事醫(yī)學(xué)科學(xué)院衛(wèi)生學(xué)環(huán)境醫(yī)學(xué)研究所;
【基金】：國(guó)家自然科學(xué)基金面上項(xiàng)目(81372947)
【分類號(hào)】：R123

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