本文選題：辛基酚 + -羥色胺��； 參考：《中國現(xiàn)代醫(yī)學(xué)雜志》2015年14期

【摘要】：目的通過觀察人肝7702細(xì)胞暴露于辛基酚(OP)后5-羥色胺(5-HT)轉(zhuǎn)運(yùn)體SERT、5-HT3b受體表達(dá)以及5-HT水平的變化,探討OP對5-HT轉(zhuǎn)運(yùn)通路和受體結(jié)合通路的影響。方法實(shí)驗(yàn)分5個(gè)組,分別為OP高(5.0μg/L)、中(1.0μg/L)、低(0.2μg/L)劑量組、鹽酸氟西汀組和空白對照組,體外細(xì)胞培養(yǎng)48 h,采用ELISA試劑盒檢測細(xì)胞上清液中5-HT含量的變化,運(yùn)用實(shí)時(shí)熒光定量PCR法檢測轉(zhuǎn)運(yùn)體SERT及5-HT3b受體的表達(dá)。結(jié)果中、高劑量組細(xì)胞上清液中5-HT含量高于空白對照組(P0.05),細(xì)胞中5-HT3b受體的表達(dá)降低(P0.05);低、中、高劑量組細(xì)胞中SERT的表達(dá)均低于空白對照組(P0.05);與鹽酸氟西汀組檢測結(jié)果一致。結(jié)論 OP可引起5-HT再攝取障礙,同時(shí)減少5-HT3b受體的表達(dá),對機(jī)體5-HT代謝網(wǎng)絡(luò)的轉(zhuǎn)運(yùn)通路及受體結(jié)合通路產(chǎn)生毒效應(yīng)作用。
[Abstract]:Objective to investigate the effect of OP on 5-HT transport pathway and receptor binding pathway by observing the changes of 5- serotonin (5-HT) transporter SERT, 5-HT3b receptor expression and 5-HT level after exposure to octyl phenol (OP) in human liver 7702 cells. Methods 5 groups were divided into OP high (5 mu g/L), medium (1 mu g/L), low (0.2 micron) dose group, and fluoxetine hydrochloride group. In the blank control group, the cell culture was 48 h in vitro, and the change of 5-HT content in the cell supernatant was detected by ELISA kit. The expression of SERT and 5-HT3b receptor in the transporter was detected by real time fluorescence quantitative PCR method. The results showed that the content of 5-HT in the supernatant of high dose group was higher than that of the blank control group (P0.05), and the expression of 5-HT3b receptor in the cell was reduced (P0.05). The expression of SERT in the low, middle and high dose groups was lower than that in the blank control group (P0.05), which was consistent with the test results of the fluoxetine hydrochloride group. Conclusion OP can cause the 5-HT reuptake barrier and reduce the expression of 5-HT3b receptor, which has a toxic effect on the transport pathway of 5-HT metabolic network and the receptor junction pathway.

【作者單位】： 華南農(nóng)業(yè)大學(xué)食品學(xué)院(廣東省食品質(zhì)量安全重點(diǎn)實(shí)驗(yàn)室);
【基金】：廣東省自然科學(xué)基金(No:S2011010001045) 廣東省科技計(jì)劃項(xiàng)目(No:2012B090600005) 農(nóng)業(yè)部農(nóng)產(chǎn)品質(zhì)量安全監(jiān)管項(xiàng)目(No:GTFP2014011)
【分類號】：R114

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 林喜燕;于瑞蓮;胡恭任;;環(huán)境激素對水生動物干擾效應(yīng)及機(jī)制研究進(jìn)展[J];環(huán)境科學(xué)與技術(shù);2009年09期

2 潘桂花,林治光,李華芳,徐韜園;抑郁癥患者血小板五羥色胺濃度的研究[J];上海精神醫(yī)學(xué);2005年03期

3 陶晶;仇劍];;雌激素對5-羥色胺能神經(jīng)元的作用機(jī)制及其臨床應(yīng)用[J];上海精神醫(yī)學(xué);2010年04期

4 張立將,尹立紅,浦躍樸;環(huán)境內(nèi)分泌干擾物檢測方法研究進(jìn)展[J];環(huán)境與職業(yè)醫(yī)學(xué);2005年02期

5 褚s，

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