本文選題：納米氧化鈰 + 大鼠肺泡巨噬細(xì)胞�。� 參考：《環(huán)境與健康雜志》2017年01期

【摘要】：目的研究納米氧化鈰對過氧化氫致大鼠肺泡巨噬細(xì)胞NR8383氧化損傷的影響。方法將對數(shù)生長期的NR8383細(xì)胞分別暴露于終濃度為5、10、20、50、100μg/ml納米氧化鈰(20 nm)懸液孵育24 h,再加入新鮮配制的終濃度為100μmol/L的過氧化氫刺激細(xì)胞2 h,另設(shè)對照(PBS)組及過氧化氫(100μmol/L)組。采用CCK-8法檢測NR8383細(xì)胞的細(xì)胞活性,采用酶標(biāo)法檢測培養(yǎng)液上清中乳酸脫氫酶(LDH)的釋放量,并測定細(xì)胞內(nèi)谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、活性氧(ROS)的水平。結(jié)果與對照組相比,過氧化氫對NR8383細(xì)胞造成氧化損傷,細(xì)胞的存活率下降25.25%。與過氧化氫組相比,5μg/ml納米氧化鈰+過氧化氫組NR8383細(xì)胞的存活率較高,而100μg/ml納米氧化鈰+過氧化氫組NR8383細(xì)胞的存活率較低,差異均有統(tǒng)計學(xué)意義(P0.05,P0.01);且隨著納米氧化鈰染毒濃度的升高,NR8383細(xì)胞的存活率呈下降的趨勢。與過氧化氫組相比,對照組NR8383細(xì)胞的LDH釋放量較低,50μg/ml納米氧化鈰+過氧化氫組NR8383細(xì)胞的LDH釋放量較高,差異有統(tǒng)計學(xué)意義(P0.05);而5、10μg/ml納米氧化鈰+過氧化氫組NR8383細(xì)胞的LDH釋放量有所降低,但差異均無統(tǒng)計學(xué)意義(P0.05)。且隨著納米氧化鈰染毒濃度的升高,NR8383細(xì)胞的LDH釋放量呈上下波動。與過氧化氫組相比,對照組NR8383細(xì)胞內(nèi)GSH的水平升高,而ROS的水平上升;5、10μg/ml納米氧化鈰+過氧化氫組NR8383細(xì)胞內(nèi)的ROS水平均下降,而10μg/ml納米氧化鈰+過氧化氫組NR8383細(xì)胞內(nèi)的GSH水平上升,差異均有統(tǒng)計學(xué)意義(P0.05)。隨著納米氧化鈰染毒濃度的升高,NR8383細(xì)胞內(nèi)的SOD、GSH呈上下波動,ROS的水平呈逐漸升高的趨勢。結(jié)論低濃度(5、10μg/ml)的納米氧化鈰顆�？梢跃徑膺^氧化氫對NR8383細(xì)胞造成的氧化損傷,對細(xì)胞起到保護(hù)作用。
[Abstract]:Objective to study the effect of nano cerium oxide on oxidative damage of NR8383 in rat alveolar macrophages induced by hydrogen peroxide.Methods the NR8383 cells in logarithmic growth phase were exposed to 50 渭 mol / L C2O _ 3 (50 渭 mol / L) suspension for 24 h, then incubated with hydrogen peroxide (100 渭 mol/L) for 2 h. The cells were divided into two groups: control group (n = 10) and hydrogen peroxide group (n = 100 渭 mol / L).The activity of NR8383 cells was detected by CCK-8 assay, the release of lactate dehydrogenase (LDH) from supernatant of culture medium was detected by enzyme labeling method, and the levels of glutathione glutathione (GSH), superoxide dismutase (SOD) and reactive oxygen species (Ros) were measured.Results compared with the control group, hydrogen peroxide caused oxidative damage to NR8383 cells and the survival rate of cells decreased 25.25%.Compared with hydrogen peroxide group, the survival rate of 5 渭 g/ml nano-cerium peroxide group was higher than that of 100 渭 g/ml nano-cerium oxide hydrogen peroxide group, while the survival rate of 100 渭 g/ml nano-cerium oxide group was lower than that of hydrogen peroxide group.The difference was statistically significant, and the survival rate of NR8383 cells decreased with the increase of the concentration of cerium oxide nanoparticles.Compared with the hydrogen peroxide group, the LDH emission of NR8383 cells in the control group was lower than that in the 50 渭 g/ml nano-cerium oxide hydrogen peroxide group, and the LDH release of the NR8383 cells in the control group was higher than that in the hydrogen peroxide group.The difference was statistically significant (P 0.05), while the release of LDH was decreased in 5 渭 g / 10 渭 g/ml hydrogen peroxide group, but there was no significant difference in LDH emission between the two groups (P 0.05).The amount of LDH released from NR8383 cells fluctuated with the increase of the concentration of cerium oxide nanoparticles.Compared with hydrogen peroxide group, the level of GSH in NR8383 cells of control group was increased, while the level of ROS in NR8383 cells of 10 渭 g/ml nano-cerium oxide group decreased.However, the level of GSH in NR8383 cells increased in 10 渭 g/ml nano-cerium oxide hydrogen peroxide group, and the difference was statistically significant (P 0.05).With the increase of the concentration of cerium oxide in NR8383 cells, the level of GSH in NR8383 cells fluctuated up and down, and the level of Ros increased gradually.Conclusion low concentration of 10 渭 g / ml cerium oxide nanoparticles can alleviate the oxidative damage caused by hydrogen peroxide on NR8383 cells and protect the cells.
【作者單位】： 內(nèi)蒙古科技大學(xué)包頭醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)與法醫(yī)學(xué)院;
【基金】：國家自然科學(xué)基金(81160341) 內(nèi)蒙古自治區(qū)自然科學(xué)基金(2015MS0868)
【分類號】：R114

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本文編號：1766761