發(fā)布時間：2018-04-16 01:39

  本文選題：鐵 + 單核苷酸多態(tài)性��； 參考：《中國疾病預防控制中心》2013年碩士論文

【摘要】：背景基因單核苷酸多態(tài)性(SNP)與人類疾病的關系早已被人們普遍關注。在營養(yǎng)學領域里,人們同樣發(fā)現與葉酸、α-生育酚、維生素D、維生素B12和鐵等營養(yǎng)素代謝與生物利用相關蛋白或酶基因的單核苷酸多態(tài)性可導致對應的營養(yǎng)素水平出現差異。本研究依據當前研究成果,重點選取了與鐵代謝相關蛋白基因rs3811647、rs7385804、rs855791、rs4820268等多態(tài)性位點進行研究,探討該4個位點的多態(tài)性與人體鐵營養(yǎng)狀況之間的關系。 目的通過比較不同等位基因人群的SF和sTfR水平,從4個鐵營養(yǎng)狀況相關的多態(tài)性位點中篩選出高風險的等位基因。為今后人群鐵營養(yǎng)干預工作探索新思路和新策略,同時也可以為個體了解自身鐵缺乏風險,為進一步開展人群干預研究提供依據。 方法選擇生活條件和營養(yǎng)背景基本相同的北京和浙江寄宿制學校學生為研究對象,隨機抽取50個血液樣本,經篩選后對其進行rs3811647、rs7385804、rs855791、rs4820268四個SNP位點的等位基因進行檢測。根據基因分型結果,按照基因型別和等位基因將研究對象分組,以鐵蛋白(SF)≥25ng/ml和轉鐵蛋白受體(sTfR)≥4.4mg/L為界值比較不同基因型別和等位基因之間SF或sTfR的差異,推測攜帶有相應等位基因發(fā)生鐵缺乏狀況的風險。 結果在本研究的48名受試者中,我們發(fā)現rs3811647、rs7385804、rs855791、rs4820268四個位點SNP分布狀況如下： 1. rs3811647:AA (6,12.5%)、GG (21,43.8%)、AG (21,43.8%); 2. rs7385804:AA (3,6.3%)、CC (20,41.7%)、AC (25,52.1%); 3. rs4820268:AA (15,31.3%)、GG (12,25.0%)、 AG (21,43.8%); 4. rs855791:AA (12,25.0%)、GG (14,29.2%)、AG (22,45.8%)。 我們還發(fā)現在三個基因的四個SNPs中,僅SF在不同基因型別之間出現差異： 1.rs855791位點的GG基因型攜帶者(X=33.1,SD=20.2)與AG基因型攜帶者(X57.7,SD=28.4)之間SF水平具有統計學差異(F=3.820,P=0.029),GG基因型攜帶者sF水平較AG基因型攜帶者低；同時A等位基因攜帶者(X=54.0,SD=28.2)與GG純合子(X=33.1,SD=20.2)之間,SF水平具有統計學差異(t=2.525,P=0.015),A等位基因攜帶者SF水平較GG純合子高； 2.rs4820268位點AA基因型攜帶者(X31.3,SD=20.6)與AG基因型攜帶者(X=60.1,SD=26.6)之間,SF水平具有統計學差異(P=0.001),AA基因型攜帶者SF水平較AG基因型攜帶者低；同時G等位基因攜帶者(X=55.5,SD=27.3)與AA純合子(X=31.3,SD=20.6)之間,SF水平具有統計學差異(t=4.047,P=0.004),G等位基因攜帶者SF水平較AA純合子高,且AA純合子與G等位基因攜帶者相比,其SF25ng/ml的OR值為4(95%CI1.10-14.49)。 結論攜帶有rs855791中G等位基因和攜帶有rs4820268中的A等位基因的個體,其SF水平異常的可能性較其他等位基因攜帶者大。rs4820268位點中AA純合子SF水平低下的風險是G等位基因攜帶者的4倍。而攜帶有rs7385804中的A等位基因的個體,其SF水平還需進一步研究。
[Abstract]:Background single nucleotide polymorphisms (SNPs) have been widely concerned about the relationship between SNPs and human diseases.In the field of nutrition, it has also been found that single nucleotide polymorphisms in nutrients such as folic acid, 偽 -tocopherol, vitamin D, vitamin B12 and iron and bioutilization of protein or enzyme genes may lead to differences in the corresponding nutrient levels.Based on the current research results, the polymorphism loci of rs3811647hrs7385804 rs855791rs4820268 were selected to study the relationship between the polymorphism of the four loci and the status of iron nutrition in human body.Objective by comparing the levels of SF and sTfR in different alleles, high risk alleles were screened from 4 polymorphic loci related to iron nutrition status.It can explore new ideas and strategies for iron nutrition intervention in the future, at the same time, it can also provide basis for individuals to understand their own iron deficiency risk and further carry out crowd intervention research.Methods A total of 50 blood samples were randomly collected from boarding schools in Beijing and Zhejiang with similar living conditions and nutritional background. The alleles at rs3811647rs7385804 rs855791rs4820268 were detected.According to the genotyping results, the subjects were grouped according to genotype and alleles. The SF or sTfR of different genotypes and alleles were compared with ferritin SF 鈮，

本文編號：1756766