本文選題：表面等離子共振(SPR) + 金納米粒子�。� 參考：《湖南農業(yè)大學》2012年碩士論文

【摘要】：阿特拉津是目前世界上使用范圍最廣、使用量最大的一種旱田除草劑,其化學性質比較穩(wěn)定,在自然環(huán)境中不易被分解,能夠長期存留在土壤和水中,污染自然環(huán)境；農作物的根系及莖中也會有殘留,危害人體健康。因此,對阿特拉津的檢測與監(jiān)控就變得十分必要。目前,有多種阿特拉津檢測方法,但這些方法都不同程度地存在著操作復雜、耗時長、樣品前處理復雜等缺點,探索新的阿特拉津檢測方法變得越來越重要。表面等離子共振(surface plasmon resonance, SPR)技術具有高靈敏度,操作簡單,檢測時間短,無須標記,耗樣量少,能實時監(jiān)測反應動態(tài)過程,只需對樣品進行簡單的前處理,也可無須純化各種生物組分等優(yōu)點,已經被國內外各實驗室廣泛應用于多個研究領域。該技術不但可檢測分析物,而且可測定分子之間相互作用的動力學常數(shù)。目前,SPR在檢測阿特拉津殘留方面做了大量的研究工作,但是對實際樣品的檢測僅以自然界的水源為主,農產品實際樣品的檢測和加標回收率很少涉及。 本文研究并建立了四種SPR檢測除草劑阿特拉津的新方法,分別是：利用氨基團置換阿特拉津的氯基團,SPR競爭檢測阿特拉津(改良競爭法)；利用金納米粒子增強SPR靈敏度直接檢測阿特拉津；利用磁納米粒子增強SPR靈敏度直接檢測阿特拉津；利用磁納米粒子增強SPR靈敏度,結合改良競爭法檢測阿特拉津。上述四種方法的最低檢測濃度依次為：10pg/mL、2pg/ml、2pg/mL和0.5pg/mL。同時利用上述四種方法對農產品中阿特拉津使用量較大的甘蔗和玉米進行實際樣品的檢測,并運用加標回收率試驗分別驗證上述所建立的四種新方法的準確度和重現(xiàn)性。實驗結果表明,本文建立的四種方法都具有很低的最低檢測濃度,回收率試驗也均呈現(xiàn)出較好的準確性和重現(xiàn)性。文中最后對SPR傳感芯片的再生方法進行研究,研究結果表明酒精-水虎魚綜合再生處理SPR舊金片的效果最好。
[Abstract]:Atrazine is one of the most widely used and used herbicides in the world at present. Its chemical properties are stable and it is difficult to be decomposed in the natural environment. It can be kept in soil and water for a long time and pollute the natural environment.There are also residues in the roots and stems of crops, endangering human health.Therefore, the detection and monitoring of atrazine become very necessary.At present, there are a variety of atrazine detection methods, but to varying degrees, these methods have the disadvantages of complex operation, long time consumption and complex sample pretreatment, so it is becoming more and more important to explore new methods for the detection of atrazine.The surface plasmon resonance (SPR) technique has the advantages of high sensitivity, simple operation, short detection time, no marking, less sample consumption, real-time monitoring of the dynamic process of reaction, and simple pretreatment of the sample.It has been widely used in many research fields at home and abroad.This technique can not only detect analytes, but also determine the kinetic constants of molecular interactions.At present, a great deal of research work has been done on the detection of atrazine residues by SPR, but the detection of real samples is mainly based on the source of water in nature, and the detection and standard recovery of agricultural products are seldom involved.In this paper, four new SPR methods for the detection of herbicide atrazine were studied and established.The results showed that atrazine was detected by using amino groups to replace atrazine (modified competition law), gold nanoparticles were used to enhance the sensitivity of SPR to detect atrazine directly.Atrazine was directly detected by magnetic nanoparticles enhanced SPR sensitivity, and atrazine was detected by magnetic nanoparticles enhanced SPR sensitivity combined with improved competition law.The lowest detection concentrations of the above four methods were: 1 10 PG / mL 2 PG / ml 2 PG / mL and 0.5 PG / mL respectively.At the same time, the above four methods were used to detect the actual samples of sugarcane and corn with large amount of atrazine in agricultural products, and the accuracy and reproducibility of the four new methods were verified by the standard adding recovery test.The experimental results show that the four methods developed in this paper have very low detection concentration, and the recovery test also shows good accuracy and reproducibility.Finally, the regeneration method of SPR sensor chip was studied. The results showed that the comprehensive regeneration of SPR was the best.
【學位授予單位】：湖南農業(yè)大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R155.5

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