本文選題：酵母源金屬硫蛋白 + 排汞��； 參考：《食品科學(xué)》2017年17期

【摘要】：目的:探討兩種酵母源金屬硫蛋白(metallothioneins,MT)-1、MT-2對(duì)氯化汞急性汞中毒小鼠排汞及對(duì)汞致肝臟及機(jī)體氧化損傷的修復(fù)作用。方法:氯化汞急性汞中毒小鼠模型通過頸部皮下注射氯化汞生理鹽水溶液構(gòu)建,急性汞中毒小鼠經(jīng)連續(xù)灌胃二巰基丙磺酸鈉(dimercaptopropansulfonate sodium,DMPS)及不同劑量(0.16、0.40、0.80 mg/(kg·d))酵母源MT 14 d后,測(cè)定小鼠體質(zhì)量、肝臟臟器系數(shù)、血液及肝臟Hg~(2+)的含量、血清谷胱甘肽過氧化物酶(glutathione peroxidase,GSH-Px)活力、超氧化物歧化酶(superoxide dismutase,SOD)活力、機(jī)體總抗氧化能力(total antioxidative capacity,T-AOC)及丙二醛(malondialdehyde,MDA)含量和兩項(xiàng)肝功能指示指標(biāo):谷丙轉(zhuǎn)氨酶(alanine aminotransferase,ALT)及谷草轉(zhuǎn)氨酶(aspartate transaminase,AST)活力。結(jié)果:相較于正常組小鼠,模型組小鼠體質(zhì)量下降、肝臟臟器系數(shù)上升,差異具有統(tǒng)計(jì)學(xué)意義(P0.05);血液及肝臟Hg~(2+)含量顯著上升(P0.05);血清GSH-Px、SOD活力及T-AOC顯著降低(P0.05),MDA含量顯著升高(P0.05);兩項(xiàng)肝功能指標(biāo)ALT及AST活力顯著升高(P0.05)。經(jīng)灌胃給藥處理后,與模型組小鼠相比,酵母源MT中、高劑量處理組及二巰基丙磺酸鈉陽(yáng)性對(duì)照組小鼠體質(zhì)量均顯著上升(P0.05),并與正常組小鼠無(wú)顯著性差異(P0.05);各給藥處理組小鼠臟器系數(shù)均較模型組小鼠顯著下降(P0.05)且呈一定的劑量-效應(yīng)關(guān)系。各灌胃給藥處理組小鼠血液及肝臟Hg~(2+)含量均不同程度下降;血清GSH-Px、SOD活力及T-AOC均不同程度回升,MDA含量及兩項(xiàng)肝功能指標(biāo)ALT及AST活力均不同程度降低。在0.16~0.80 mg/kg劑量范圍內(nèi),高劑量酵母源MT給藥效果最佳,與其他灌胃給藥處理組差異顯著(P0.05)。結(jié)論:兩種酵母源MT對(duì)急性汞中毒小鼠具有良好的結(jié)合機(jī)體內(nèi)游離Hg~(2+)及修復(fù)肝臟損傷及機(jī)體氧化損傷作用,且作用效果與給藥劑量呈正相關(guān)。
[Abstract]:Objective: to investigate the effects of two kinds of metallothioneins metallothioneins (MT-1) MT-2 on mercury excretion and oxidative damage induced by mercury in mice with acute mercury chloride poisoning.Methods: the mouse model of acute mercuric chloride poisoning was established by subcutaneous injection of mercuric chloride with normal saline solution. The mice were fed with sodium dimercaptopropansulfonate sodium dimercaptopropansulfonate (DMPS) and different dosages of 0.160.400.400.80 mg/(kg DX for 14 days.The body weight, liver organ coefficient, blood and liver Hg~(2 content, glutathione peroxidase (GSH-Px) activity and superoxide dismutase (SOD) activity were measured.Total antioxidative capacityTAOC), malondialdehyde (MDA), malondialdehyde (MDA) and two indicators of liver function: alanine aminotransferase (alt) and aspartate transaminase (AST).Results: compared with the normal group, the body mass of the model group decreased and the liver organ coefficient increased.The difference was statistically significant (P 0.05); the content of Hg~(2 in blood and liver increased significantly (P 0.05); the activity of serum GSH-PxX SOD and T-AOC decreased significantly (P 0.05); the content of ALT and AST increased significantly (P 0.05); the activity of ALT and AST increased significantly (P 0.05).Compared with the model group, the yeast source MT was treated by gavage.The body weight of mice in high dose treatment group and sodium dimercaptopropanesulfonate positive control group was significantly higher than that in normal group, and there was no significant difference between the two groups, and the visceral coefficient of each administration group was significantly lower than that of model group mice (P 0.05).A certain dose-effect relationship.The contents of Hg~(2 in blood and liver were decreased in each group, the activity of GSH-PxX SOD in serum and the activity of T-AOC were increased in different degree, and the activities of ALT and AST in two indexes of liver function were decreased in different degree.Within the dosage range of 0.16 ~ 0.80 mg/kg, the high dose yeast source MT had the best effect, and there was a significant difference between the high dose yeast source MT and other gavage groups (P 0.05).Conclusion: the two kinds of yeast source MT have good effects on the combination of free Hg~(2 and repair of liver injury and oxidative injury in mice with acute mercury poisoning, and the effect is positively correlated with the dosage of the drug.
【作者單位】： 黑龍江八一農(nóng)墾大學(xué)食品學(xué)院;國(guó)家雜糧工程技術(shù)研究中心;青島瑯琊臺(tái)集團(tuán)股份有限公司;
【基金】：黑龍江省博士后落地資助項(xiàng)目(LBH-Q15116) 黑龍江省新世紀(jì)優(yōu)秀人才培養(yǎng)計(jì)劃項(xiàng)目(1254-NCET-015) 黑龍江八一農(nóng)墾大學(xué)青年創(chuàng)新人才項(xiàng)目(CXRC2016-15);黑龍江八一農(nóng)墾大學(xué)研究生創(chuàng)新科研項(xiàng)目(YJSCX2017-Y56;YJSCX2017-Y57) 新世紀(jì)優(yōu)秀人才項(xiàng)目(1254-NCET-015) 2015年度國(guó)家星火計(jì)劃項(xiàng)目(2015GA670008)
【分類號(hào)】：R151

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