本文選題：白藜蘆醇 + SD妊娠大鼠��； 參考：《四川農(nóng)業(yè)大學(xué)》2012年碩士論文

【摘要】：本研究旨在考察白藜蘆醇(resveratrol, RES)對肥胖妊娠SD大鼠胚胎存活的影響,采用Diquat為應(yīng)激源研究母體灌服RES對后代抗氧化能力的影響。試驗(yàn)包括以下兩部分： 試驗(yàn)一白藜蘆醇對肥胖妊娠SD大鼠胚胎存活和抗氧化能力影響 本試驗(yàn)選用40只SPF級雌性SD大鼠隨機(jī)分為2個(gè)處理組：高脂日糧組(20%脂肪)和標(biāo)準(zhǔn)日糧組(7%脂肪),每個(gè)處理20個(gè)重復(fù),每個(gè)重復(fù)1只SD大鼠。SD大鼠適應(yīng)1周后,供給處理日糧,飼喂8周后成功構(gòu)建營養(yǎng)性肥胖模型(高脂日糧組雌鼠體重量為標(biāo)準(zhǔn)日糧組雌鼠體重量的20%,記為實(shí)驗(yàn)性肥胖大鼠),隨后大鼠雌：雄=1：1合籠配種,檢查陰戶發(fā)現(xiàn)陰栓,則記為妊娠當(dāng)天E(0)。在此基礎(chǔ)上,選取正常妊娠鼠和肥胖妊娠鼠各20只,采用2×2因子,設(shè)計(jì)四個(gè)處理組,分別是高脂日糧組、高脂日糧灌服白藜蘆醇(200mg/kg.bw)組、標(biāo)準(zhǔn)日糧組、標(biāo)準(zhǔn)日糧灌服白藜蘆醇(200mg/kg.bw)組。每個(gè)處理妊娠大鼠各10只,每個(gè)重復(fù)1只,妊娠第3天至17天灌服RES,于妊娠第18天每個(gè)處理分別選取4只妊娠大鼠屠宰取樣,考察RES對肥胖妊娠SD大鼠繁殖成績及抗氧化能力的影響。結(jié)果表明：1)試驗(yàn)各組胎兒重和胎盤重差異不顯著(P0.05),但高脂口糧灌服RES顯著提高了母體妊娠第18天的胚胎存活率(p0.05)。2)與高脂日糧相比,高脂日糧灌服RES組甘油三酯(TG)濃度降低53%(p0.05)、游離脂肪酸(FFA)降低28%(P0.05)。3)與高脂日糧組相比,高脂日糧灌服RES能顯著提高母體肝臟、胎盤、胎肝的總抗氧化能力(T-AOC)和谷胱甘肽過氧化物酶(GSH-Px)活性(P0.05),降低丙二醛(MDA)濃度(P0.05),但對母體肝臟、胎盤、胎肝的總超氧化物歧化酶(T-SOD)及過氧化氫酶(CAT)活性沒有顯著影響。 試驗(yàn)二妊娠期母鼠白藜蘆醇攝入對后代抗氧化能力的影響 本試驗(yàn)采用2×2×2因子設(shè)計(jì),分別選用試驗(yàn)一四個(gè)處理組剩余的6只妊娠大鼠的分娩子鼠,直至哺乳子鼠22天斷奶,斷奶后代分別按高脂日糧、高脂日糧灌服白藜蘆醇、標(biāo)準(zhǔn)日糧、標(biāo)準(zhǔn)日糧灌服白藜蘆醇進(jìn)行分組,每個(gè)處理8個(gè)重復(fù),每個(gè)重復(fù)1只,子鼠均飼喂同一標(biāo)準(zhǔn)日糧,以Diquat為應(yīng)激源,從第23天開始,每間隔一天上午8：00腹腔注射Diquat (8mg/kg.BW,用0.9%的滅菌生理鹽水稀釋),共7次,對照組注射相同劑量的滅菌生理鹽水。斷奶后第14天早晨眼眶靜脈采血,分離血清,測定其抗氧化指標(biāo)。結(jié)果表明：腹腔注射Diquat應(yīng)激源后,與高脂日糧組相比,高脂日糧灌服白藜蘆醇組的子鼠斷奶后第7天和14天體重分別增加12%和25%,血清T-AOC和CAT分別提高33%和37%(P0.05),MDA濃度降低16%(P0.05),標(biāo)準(zhǔn)日糧灌服白藜蘆醇組子鼠斷奶后第7天和14天體重分別增加1%和3%,血清T-AOC和CAT提高1.5%和1%,MDA濃度降低3%。 上述結(jié)果表明,RES具有降低肥胖妊娠SD大鼠體重的趨勢,顯著改善母體、胎盤和胎兒抗氧化能力,提高胚胎存活率；同時(shí)母體妊娠期灌服RES可提高后代抗氧化能力。
[Abstract]:The purpose of this study was to investigate the effects of resveratrol resveratrolle (res) on the embryo survival of obese SD rats. Diquat was used as the stress source to study the effect of maternal administration of RES on the antioxidant capacity of offspring.The experiment consists of the following two parts:Effects of resveratrol on embryo survival and antioxidant capacity in obese SD rats with pregnancyIn this experiment, 40 SPF female SD rats were randomly divided into two groups: high fat diet group (20% fat) and standard diet group (7% fat).After feeding for 8 weeks, a nutritional obesity model was successfully established (the weight of female rats in the high-fat diet group was 20% of that in the standard diet group, recorded as experimental obese rats, and then the female: male 1: 1 cage was bred, and the vulva was found to have negative embolism.It was recorded as the day of pregnancy.On this basis, 20 normal pregnant rats and 20 obese pregnant rats were selected, and 2 脳 2 factors were used to design four treatment groups: high fat diet group, high fat diet fed with resveratrol 200 mg / kg 路bww group, standard diet group.The standard diet was fed with resveratrol 200 mg / kg 路bw.Ten pregnant rats were treated with RES3 to 17 days of gestation, and 4 pregnant rats were slaughtered on the 18th day of pregnancy.To investigate the effect of RES on reproductive performance and anti-oxidation ability of obese pregnant SD rats.The results showed that there was no significant difference in fetal weight and placental weight among the three groups, but the embryo survival rate on the 18th day of gestation was significantly increased by RES administration with high fat ration compared with the high fat diet.Compared with the high fat diet group, the concentration of triglyceride (TG) and free fatty acid (FFA) in the high fat diet group were decreased by 53% and 28% (P 0.05) 路3 respectively. Compared with the high fat diet group, the high fat diet administration of RES could significantly improve the maternal liver and placenta.The total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) activity of fetal liver (T- AOC) and glutathione peroxidase (GSH-Px) activity (P0. 05) decreased the concentration of malondialdehyde (MDA) (P0. 05), but had no significant effect on the activities of total superoxide dismutase (T-SOD) and catalase (CAT) in maternal liver, placenta and fetal liver.Effect of resveratrol intake on antioxidant capacity of offspring in pregnant ratsIn this experiment, 2 脳 2 脳 2 factor design was used to test the remaining 6 pregnant rats in four treatment groups, which were weaned until 22 days after weaning. After weaning, they were fed with high fat diet, high fat diet, resveratrol, standard diet, respectively.Resveratrol was administered with standard diet, 8 replicates in each treatment, and 1 repeats in each treatment. The offspring were fed with the same standard diet. Diquat was used as the stress source from the 23rd day.Diquat 8 mg / kg 路BW was injected intraperitoneally at 8:00 every day and diluted with 0.9% sterilized saline for 7 times. The control group was injected with the same dose of sterilized saline.On the 14th day after weaning, blood was collected from orbital vein, serum was isolated and its antioxidant index was measured.The results showed that: after intraperitoneal injection of Diquat stress source, compared with high-fat diet group,In resveratrol group, the body weight increased by 12% and 25% on the 7th and 14th day after weaning, the serum T-AOC and CAT increased by 33% and 37%, respectively. The concentration of T-AOC decreased by 16% and decreased by 16%. The rats fed with standard diet on the 7th and 14th day after weaning were given resveratrol.The body weight increased by 1% and 3%, the serum T-AOC and CAT increased by 1.5% and the concentration of MDA decreased by 3%.The results indicated that res had the tendency to decrease the body weight of obese SD rats, improve the antioxidation ability of mother, placenta and fetus, and increase the survival rate of embryos, and the maternal administration of RES during pregnancy could improve the antioxidant ability of offspring.
【學(xué)位授予單位】：四川農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R151

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