本文選題：鋁　切入點：-二甲基--羥基--吡啶酮　出處：《環(huán)境與健康雜志》2015年11期

【摘要】：目的探討1,2-二甲基-3-羥基-4-吡啶酮(deferipone,DFP)和�；撬崧�(lián)合作用對染鋁大鼠大腦皮層抗氧化系統(tǒng)的影響。方法將70只健康SPF級雄性Wistar大鼠按體重隨機(jī)分為7組,以灌胃方式染毒,其中,陰性對照組給予1 ml生理鹽水,連續(xù)8周;鋁染毒組、�；撬岣深A(yù)組、低劑量DFP干預(yù)組、高劑量DFP干預(yù)組、牛磺酸+低劑量DFP干預(yù)組和�；撬�+高劑量DFP干預(yù)組在前4周分別給予281.40 mg/kg三氯化鋁溶液,后4周分別給予1 ml生理鹽水、400 mg/kg牛磺酸、13.82 mg/kg DFP、27.44 mg/kg DFP、400 mg/kg�；撬�、400 mg/kg牛磺酸;�；撬�+低劑量DFP干預(yù)組和�；撬�+高劑量DFP干預(yù)組給予400 mg/kg牛磺酸6 h后分別給予13.82、27.44 mg/kg DFP,每天1次。測定大鼠大腦皮層SOD、GSH-Px活力和MDA含量。結(jié)果與陰性對照組比較,鋁染毒組大鼠大腦皮層SOD和GSH-Px活力均較低,而MDA含量較高,差異有統(tǒng)計學(xué)意義(P0.05)。與鋁染毒組相比,在單獨干預(yù)組中,僅高劑量DFP干預(yù)組可以顯著提高SOD和GSH-Px活力,降低MDA的含量;�；撬岣深A(yù)組可以顯著提高GSH-Px的活力水平以及降低MDA含量。在DFP和�；撬岬穆�(lián)合作用下,大鼠大腦皮層的SOD和GSH-Px活力水平以及MDA含量均基本恢復(fù)正常。其中,與牛磺酸干預(yù)組和低劑量DFP干預(yù)組比較,�；撬�+DFP干預(yù)組可以顯著提高SOD和GSH-Px活力,降低MDA含量;且以�；撬�+低劑量DFP干預(yù)組尤為顯著。結(jié)論在本實驗劑量范圍內(nèi),�；撬岷虳FP聯(lián)合干預(yù)可以對染鋁大鼠大腦皮層的抗氧化系統(tǒng)起到保護(hù)作用,且效果優(yōu)于�；撬峄駾FP單獨干預(yù)。
[Abstract]:Objective to investigate the effects of 1-dimethyl-3-hydroxy-4-pyridine-deferipone (DFPFP) and taurine on the antioxidation system in the cerebral cortex of rats exposed to aluminum.Methods 70 healthy SPF grade male Wistar rats were randomly divided into 7 groups according to their body weight. Among them, the negative control group was given 1 ml of normal saline for 8 weeks, the aluminum group, taurine intervention group, and low dose DFP intervention group.High dose DFP intervention group, taurine low dose DFP intervention group and taurine high dose DFP intervention group were given 281.40 mg/kg aluminum trichloride solution in the first 4 weeks, respectively.At the end of 4 weeks, 1 ml normal saline for 400 mg/kg taurine was administrated with 13.82 mg/kg DFPX 27.44 mg/kg mg/kg taurine and 400 mg/kg taurine respectively.Taurine low dose DFP intervention group and taurine high dose DFP intervention group were treated with taurine for 6 h, 13.82 mg/kg 27.44 mg/kg DFP, once a day, respectively.The activity of GSH-Px and the content of MDA in cerebral cortex of rats were measured.Results compared with the negative control group, the activity of SOD and GSH-Px in the cerebral cortex of rats exposed to aluminum was lower, while the content of MDA was higher, the difference was statistically significant (P 0.05).Compared with aluminum exposure group, only high dose DFP group could significantly increase the activity of SOD and GSH-Px and decrease the content of MDA, while taurine group could significantly increase the level of GSH-Px activity and decrease the content of MDA.Under the combined action of DFP and taurine, the activities of SOD and GSH-Px and the content of MDA in the cerebral cortex of rats returned to normal.Compared with taurine intervention group and low dose DFP intervention group, taurine DFP intervention group could significantly increase SOD and GSH-Px activity and decrease MDA content, especially in taurine low dose DFP intervention group.Conclusion the combined intervention of taurine and DFP can protect the antioxidation system in the cerebral cortex of aluminized rats, and the effect is better than that of taurine or DFP alone.
【作者單位】： 山東大學(xué)公共衛(wèi)生學(xué)院衛(wèi)生檢驗學(xué)系;
【基金】：山東省自然科學(xué)基金(ZR2013CM043)
【分類號】：R114
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本文編號：1714978