本文選題：快速檢測(cè)　切入點(diǎn)：多重實(shí)時(shí)熒光定量PCR　出處：《現(xiàn)代預(yù)防醫(yī)學(xué)》2016年22期

【摘要】：目的了解成都地區(qū)市售辣椒粉受霉菌污染的狀況,探討裸磁珠-多重實(shí)時(shí)熒光定量PCR方法快速定量檢測(cè)霉菌數(shù)量的實(shí)用性。方法于成都市區(qū)隨機(jī)選取農(nóng)貿(mào)市場(chǎng),采集辣椒樣品40份,按照國(guó)標(biāo)GB4789.15-2010對(duì)樣品進(jìn)行霉菌計(jì)數(shù),純化后以鏡下特征及菌落特征進(jìn)行菌種鑒定及分類計(jì)數(shù),同時(shí)采用裸磁珠-實(shí)時(shí)熒光PCR方法檢測(cè)霉菌數(shù)量,通過(guò)與傳統(tǒng)方法比較,探討裸磁珠-實(shí)時(shí)熒光PCR方法的實(shí)用性。結(jié)果所采集樣品的霉菌污染均數(shù)達(dá)到了5.1×103CFU/g;對(duì)曲霉屬、青霉屬及鐮刀菌屬分類計(jì)數(shù),裸磁珠-多重實(shí)時(shí)熒光定量PCR的檢測(cè)法計(jì)數(shù)結(jié)果幾何均數(shù)分別為6.1×108、4.0×101及2.9×106CFU/g,此結(jié)果與傳統(tǒng)培養(yǎng)計(jì)數(shù)法2.0×103、1.1×102及2.1×101CFU/g相比有顯著增加,兩者結(jié)果具有統(tǒng)計(jì)學(xué)差異。結(jié)論成都市售辣椒粉受霉菌污染嚴(yán)重,對(duì)消費(fèi)者健康構(gòu)成較大威脅。相比傳統(tǒng)培養(yǎng)法,裸磁珠-多重實(shí)時(shí)熒光定量PCR方法節(jié)約時(shí)間、靈敏度高且受稀釋等操作的影響較小、受樣本成分及樣本中其他霉菌干擾較小,具有很高的應(yīng)用價(jià)值。
[Abstract]:Objective to investigate the situation of pepper powder contaminated by mold in Chengdu area, and to explore the practicability of rapid quantitative detection of mold by naked magnetic bead and multiplex real-time fluorescent quantitative PCR. Forty pepper samples were collected and counted according to the national standard GB4789.15-2010. After purification, the fungus species were identified and classified by microscopic and colony characteristics. At the same time, the number of mold was detected by naked magnetic bead and real-time fluorescence PCR. Compared with the traditional method, the practicability of the naked magnetic bead real-time fluorescence PCR method was discussed. Results the mean of mold contamination of the collected samples reached 5.1 脳 103 CFU / g, and the taxonomic counts of Aspergillus, Penicillium and Fusarium were counted. The geometric mean values of bare magnetic bead and multiplex real-time fluorescent quantitative PCR were 6.1 脳 108U 4.0 脳 101 and 2.9 脳 10 6 CFU / g, respectively, which were significantly higher than that of traditional culture counting method (2.0 脳 103n 1.1 脳 102 and 2.1 脳 101CFU/g). Conclusion the chilli powder sold in Chengdu is seriously polluted by mold and poses a great threat to consumer health. Compared with the traditional culture method, the naked magnetic bead multiplex real-time fluorescent quantitative PCR method saves time. It has high sensitivity and less influence by dilution operation, and is less interfered by sample composition and other molds in the sample, so it has high application value.
【作者單位】： 四川大學(xué)華西公共衛(wèi)生學(xué)院(華西第四醫(yī)院);廣東省珠海市疾病預(yù)防控制中心;四川省疾病預(yù)防控制中心;浙江中醫(yī)藥大學(xué);四川省食品安全監(jiān)測(cè)與風(fēng)險(xiǎn)評(píng)估重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金重點(diǎn)項(xiàng)目"水產(chǎn)品/農(nóng)產(chǎn)品/香料中化學(xué)污染物/產(chǎn)毒真菌的快速檢測(cè)和食品中有害物質(zhì)健康危害的安全性評(píng)價(jià)研究"(No.81030053)資助
【分類號(hào)】：R155.5

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