本文選題：蝦青素　切入點：食管癌　出處：《鄭州大學》2017年碩士論文　論文類型：學位論文

【摘要】：目的探討蝦青素油劑抑制食管癌的效果,并初步闡明其抑制食管癌發(fā)生的機制。方法取84只4-6周齡F344雄性大鼠,隨機分為6組,即對照組A[0.25mg/kg·body weight(BW)],[雙蒸水+20%二甲基亞砜(Dimethyl sulfoxid,DMSO)],蝦青素低劑量組B(10mg/kg·BW)、中劑量組C(50mg/kg·BW)、高劑量組D(100mg/kg·BW)、溶劑組E(紅花籽油)、暴露組F[N-甲基芐基亞硝胺,(N-nitrosomethylbenzylamine,NMBzA)+DMSO]。B、C、D、E、F組皮下注射NMBzA(0.25mg/kg·BW)(5周),B、C、D組灌胃蝦青素油劑(25周),每周三次。實驗第25周、35周分別處死大鼠,腹主動脈采血,HE染色觀察食管組織形態(tài)結(jié)構(gòu),酶聯(lián)免疫法檢測血清超氧化物歧化酶(Superoxide dismutase,T-SOD)、谷胱甘肽過氧化物酶(Glutathione peroxidases,GSH-PX)活性、丙二醛(Malondialdehyde,MDA)含量及血漿過氧化氫酶(Catalase,CAT)活性、總抗氧化能力(Total antioxidant capacity,TAOC)及血清轉(zhuǎn)化生長因子β1(Transforming Growth Factor-beta 1,TGF-β1)水平。用SPSS 21.0統(tǒng)計軟件對實驗數(shù)據(jù)進行分析,檢驗水準α=0.05。結(jié)果(1)大鼠食管肉眼觀腫瘤發(fā)生情況:第25周,溶劑組、暴露組發(fā)生率均高于對照組及中劑量組,暴露組發(fā)生率高于低劑量組。第35周,溶劑組和暴露組發(fā)生率均高于對照組。差異均具有統(tǒng)計學意義(P0.05)(2)大鼠食管黏膜上皮組織學發(fā)現(xiàn):第25周和35周,低劑量組、高劑量組、溶劑組及暴露組組織學形態(tài)與對照組不同,低劑量組及高劑量組多見異常增生、溶劑組及暴露組多見腫瘤,對照組僅見正常上皮和單純增生。第25周,中劑量組僅見正常上皮和單純增生,與低劑量組、高劑量組、溶劑組及暴露組組織學形態(tài)不同,但第35周中劑量組可見異常增生和腫瘤,與暴露組組織學形態(tài)不同。差異均具有統(tǒng)計學意義(P0.05)。(3)大鼠氧化應(yīng)激水平檢測:第25周,對照組、中劑量組血清GSH-PX活性均高于暴露組。高劑量組和對照組血漿CAT活性均高于暴露組。暴露組血清MDA含量高于低劑量組。中劑量組血漿TAOC水平均高于暴露組及溶劑組。差異均有統(tǒng)計學意義(P0.05)。(4)大鼠血清TGF-β1水平檢測:第25周,中劑量組及暴露組均高于低劑量組及對照組。第35周,溶劑組及暴露組水平高于對照組,差異均有統(tǒng)計學意義(P0.05)。(5)大鼠體重及脾臟重量:第25周,與對照組、低劑量組、中劑量組相比,高劑量及暴露組體重較輕;中劑量組大鼠脾臟重量高于溶劑組及暴露組,差異均有統(tǒng)計學意義(P0.05)。結(jié)論適當劑量蝦青素油劑能夠抑制NMBzA誘導(dǎo)的大鼠食管癌發(fā)生,可能通過提高大鼠抗氧化能力,提高大鼠TGF-β1的水平而抑制食管癌的發(fā)生。
[Abstract]:Objective to investigate the inhibitory effect of astaxanthin oil on esophageal carcinoma and its mechanism. Methods 84 F344 male rats aged 4-6 weeks were randomly divided into 6 groups. Control group A [0.25 mg / kg 路body weighted BW], [double distilled water 20% Dimethyl sulfoxidido], astaxanthin low dose group B10 mg / kg 路BWN, middle dose group C0 50 mg / kg 路BWW, high dose group D 100 mg / kg 路BW, solvent group E, exposure group F [N-nitrothylbenzylamine NMBzA]. The rats were killed at the 25th week, 35 weeks after the experiment. The morphological structure of esophagus was observed by HE staining of abdominal aorta. The activities of superoxide dismutase T-SODX, glutathione peroxidase peroxidase (Glutathione peroxidase) GSH-PX, malondialdehyde (malondialdehyde) malondialdehyde (MDA) and plasma catalase catalase (CAT) in serum were detected by enzyme-linked immunosorbent assay (Elisa). Total antioxidant capacity (TAOC) and serum transforming Growth Factor-beta (TGF- 尾 1) levels of TGF- 尾 1. The experimental data were analyzed with SPSS 21.0 software to test the level of 偽 0. 05. The results showed that the incidence of naked esophageal neoplasms in rats: 25 weeks, solvent group, TGF- 尾 1, TGF- 尾 1, TGF- 尾 1, TGF- 尾 1, TGF- 尾 1, TGF- 尾 1, TGF- 尾 1, TGF- 尾 1 and TGF- 尾 1. The incidence of exposure group was higher than that of control group and middle dose group, and the incidence of exposure group was higher than that of low dose group. The incidence of esophageal mucosal epithelium in solvent group and exposure group was higher than that in control group. The difference was statistically significant (P 0.05): at the 25th week and the 35th week, the low dose group and the high dose group, the lower dose group, the higher dose group, the lower dose group, the lower dose group and the higher dose group. The histological morphology of solvent group and exposure group was different from that of control group. Abnormal hyperplasia was found in low dose group and high dose group, tumor in solvent group and exposure group, normal epithelium and simple hyperplasia in control group. Normal epithelium and simple hyperplasia were observed in the middle dose group, which were different from those in the low dose group, the high dose group, the solvent group and the exposure group, but abnormal hyperplasia and tumor were found in the middle dose group at the 35th week. The difference was statistically significant (P 0.05)) the level of oxidative stress was detected in rats: at the 25th week, the control group, the control group, and the control group. The serum GSH-PX activity in the middle dose group was higher than that in the exposure group, the plasma CAT activity in the high dose group and the control group was higher than that in the exposure group, the serum MDA level in the exposure group was higher than that in the low dose group, and the plasma TAOC level in the middle dose group was higher than that in the exposure group and solvent group. The levels of TGF- 尾 1 in serum of rats were detected at the 25th week. At the 35th week, the level of the solvent group and the exposure group were higher than that of the control group, and the difference was statistically significant (P < 0.05) the weight and spleen weight of the rats: at the 25th week, it was higher than that in the control group and the low-dose group. The weight of spleen in the middle dose group was higher than that in the solvent group and exposure group, and the difference was statistically significant (P 0.05). Conclusion the appropriate dosage of astaxanthin oil can inhibit the occurrence of esophageal carcinoma induced by NMBzA in rats. It is possible to inhibit the occurrence of esophageal cancer by increasing the level of TGF- 尾 1 and anti-oxidation ability in rats.
【學位授予單位】：鄭州大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R151

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