發(fā)布時(shí)間：2018-03-14 19:29

  本文選題：豬皮　切入點(diǎn)：膠原蛋白　出處：《四川農(nóng)業(yè)大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：本論文以豬皮為原料提取膠原蛋白并制備出膠原蛋白酶解物,分別對(duì)其物理性質(zhì)、不同分子量肽段組分的體外抗氧化活性、體內(nèi)抗氧化作用以及作為抗氧化劑在豬油及豬肉糜中的抗氧化效果進(jìn)行研究,為進(jìn)一步利用豬皮膠原蛋白開(kāi)發(fā)抗氧化肽提供試驗(yàn)基礎(chǔ)。 1.分析豬皮膠原蛋白酶解物的溶解性、吸油性、乳化性、乳化穩(wěn)定性、起泡性及起泡穩(wěn)定性等物理性質(zhì),為其在食品、保健品等領(lǐng)域的有效利用提供一定的依據(jù)。 2.利用不同膜截留分子量的超濾離心管對(duì)豬皮膠原蛋白酶解物進(jìn)行分離,得到分子量大于10kDa、5kDa-10kDa、3kDa-5kDa和小于3kDa的4個(gè)分子量肽段的組分,以氧自由基(02-·)清除活性、羥自由基(·OH)清除活性、DPPH·自由基清除活性、還原力、脂質(zhì)過(guò)氧化抑制能力為指標(biāo),測(cè)定不同分子量肽段組分及酶解液的抗氧化能力。結(jié)果顯示,不同分子量范圍的豬皮膠原蛋白酶解物均具有一定的抗氧化能力,且隨著濃度的增大,其抗氧化活性越強(qiáng)。其中,酶解液對(duì)O：-·清除活性最好,分子量5kDa-10kDa組分對(duì)·OH清除活性、還原力和脂質(zhì)過(guò)氧化抑制能力最好,3kDa-5kDa組分對(duì)DPPH·自由基清除活性最好。 3.將昆明小鼠隨機(jī)分成5組,分別為：空白組、衰老模型組、豬皮膠原蛋白酶解物低劑量組(100mg/kg).豬皮膠原蛋白酶解物中劑量組(200mg/kg)和豬皮膠原蛋白酶解物高劑量組(400mg/kg)。除空白組外,其他各組均頸背部皮下注射D-半乳糖,飼養(yǎng)42d后,以小鼠血清、肝臟和大腦中的超氧化物歧化酶(SOD)、谷胱甘肽過(guò)氧化物酶(GSH-PX)的活性及丙二醛(MDA)含量為指標(biāo),考察豬皮膠原蛋白酶解物在小鼠體內(nèi)的抗氧化性能。結(jié)果表明,與空白組小鼠相比,衰老模型組小鼠的血清、肝臟、大腦中的SOD、GSH-PX活性明顯降低,MDA含量明顯升高；豬皮膠原蛋白酶解物能提高小鼠血清、肝臟和大腦中的SOD和GSH-PX活性,降低MDA含量,并呈現(xiàn)出一定的劑量效應(yīng)關(guān)系。豬皮膠原蛋白酶解物對(duì)D-半乳糖致衰老小鼠具有一定的抗氧化作用。 4.將不同量的豬皮膠原蛋白酶解物分別添加到豬油及豬肉糜中,考察其對(duì)豬油及豬肉糜氧化的抑制作用。結(jié)果表明,豬皮膠原蛋白酶解物對(duì)豬油及豬肉糜均具有一定的抗氧化作用,且隨著添加量的增加其抗氧化能力也增強(qiáng),當(dāng)添加量為2%時(shí),其抗氧化效果最好,但其效果略低于0.02%的叔丁基羥基茴香醚(BHA)。
[Abstract]:In this paper, collagen was extracted from pig skin and collagen proteolysis was prepared. The physical properties and antioxidant activity of different molecular weight peptide fractions in vitro were studied. The antioxidative effect in vivo and antioxidant activity in pork and lard were studied, which provided the experimental basis for the further development of antioxidant peptides by using porcine skin collagen. 1. The solubility, oil absorption, emulsification, emulsification stability, foaming property and foaming stability of porcine skin collagen protease hydrolysate were analyzed, which provided some basis for its effective utilization in food and health products. 2. The porcine skin collagen proteolytic compounds were separated by ultrafiltration centrifuge tubes with different molecular weight, and the four molecular weight peptides with molecular weight larger than 10kDa5kDa-10kDa-5kDa and less than 3kDa were obtained. Hydroxyl radical scavenging activity DPPH 路free radical scavenging activity, reducing capacity and inhibiting capacity of lipid peroxidation were used as indexes to determine the components of different molecular weight peptides and the antioxidant ability of enzymatic hydrolysis solution. The antioxidation activity of porcine skin collagen protease hydrolysates with different molecular weight ranges was higher with the increase of concentration, among them, the enzymatic hydrolysis solution had the best scavenging activity against O:-路, and the molecular weight of 5kDa-10kDa component had the best scavenging activity against 路OH. Reduction and lipid peroxidation inhibition were the best. The 3kDa-5kDa component had the best scavenging activity against DPPH 路free radical. 3. Kunming mice were randomly divided into five groups: blank group, aging model group, low dose group of porcine skin collagen proteolytic substance (100mg / kg), middle dose group (200mg / kg) and high dose group (400mg / kg). Dgalactose was injected subcutaneously into the neck and back of other groups. After 42 days of feeding, the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and the content of malondialdehyde (MDA) in serum, liver and brain of mice were used as indexes. The antioxidative activity of porcine collagen protease hydrolysate in mice was investigated. The results showed that the activity of SOD- GSH-PX in serum, liver and brain of aging model group was significantly lower than that of blank group. Porcine skin collagen proteolytic agent can increase the activity of SOD and GSH-PX in serum, liver and brain of mice, and decrease the content of MDA. The porcine skin collagen proteolysis had a certain antioxidant effect on aging mice induced by D-galactose. 4. The different amounts of porcine skin collagen protease hydrolysates were added to the pork and lard respectively, and the inhibitory effect on the oxidation of the lard and the pork surimi was investigated. Porcine skin collagen protease hydrolysate had antioxidation effect on both lard and pork chilli, and its antioxidant activity also increased with the increase of the amount of pig skin collagen protease, and the antioxidant effect was the best when the amount of collagen protease hydrolysate was 2%. But its effect is slightly lower than that of uncle Ding Ji hydroxyl anisole (0.02%).
【學(xué)位授予單位】：四川農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R151

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本文編號(hào)：1612584