本文選題：稀土元素　切入點：鑭　出處：《中國公共衛(wèi)生》2015年01期 　論文類型：期刊論文

【摘要】：目的探討亞慢性氯化鑭(La Cl3)暴露對子代大鼠海馬組織核轉(zhuǎn)錄因子-κB(NF-κB)表達(dá)的影響。方法健康成年雌性Wistar大鼠24只,隨機(jī)分為對照組及0.25%、0.5%、1.0%La Cl3組,染鑭組仔鼠自雌鼠受孕至斷乳后1個月通過自由飲水?dāng)z入鑭;利用免疫組化法檢測海馬p-IKKα/β的分布和表達(dá);應(yīng)用Western blot法檢測海馬組織p-IκBα和p-NF-κBP65的表達(dá)。結(jié)果 0.25%、0.5%、1.0%La Cl3組仔鼠海馬CA1區(qū)p-IKKα/β表達(dá)分別為(54.72±10.04)、(40.73±4.90)、(32.99±5.42),CA3區(qū)表達(dá)分別為(64.80±8.27)、(47.78±4.92)、(36.17±7.71),DG區(qū)表達(dá)分別為(303.67±34.78)、(166.83±29.8)、(87.01±17.40);CA1、CA3、DG區(qū)各染鑭組仔鼠海馬3區(qū)的p-IKKα/β表達(dá)與對照組比較,除DG區(qū)0.25%La Cl3組外其他各染鑭組仔鼠海馬p-IKKα/β表達(dá)均低于對照組(P0.05);0.25%、0.5%、1.0%La Cl3組仔鼠海馬p-IκBα和p-NF-κBp65蛋白表達(dá)IDV值分別為(0.58±0.06)和(0.46±0.06)、(0.49±0.07)和(0.36±0.08)、(0.36±0.09)和(0.25±0.08),均低于對照組的(0.68±0.05)和(0.56±0.08)(P0.05),仔鼠海馬p-IκBα和p-NF-κBp65蛋白表達(dá)均與La Cl3染毒劑量呈負(fù)相關(guān)(r=-0.994、-0.978,P0.05)。結(jié)論亞慢性La C13染毒可引起子代大鼠海馬細(xì)胞NF-κB活性降低,可能是鑭引起學(xué)習(xí)記憶能力下降的機(jī)制之一。
[Abstract]:Objective to investigate the effect of subchronic lanthanum chloride (La Cl 3) exposure on the expression of nuclear transcription factor 魏 B (NF- 魏 B) NF- 魏 B in hippocampus of offspring rats. Methods Twenty-four healthy adult female Wistar rats were randomly divided into two groups: the control group and the 0.25g / 0.50g / 1.0La Cl3 group. In lanthanum group, lanthanum was ingested through free drinking water one month after weaning, and the distribution and expression of p-IKK 偽 / 尾 in hippocampus were detected by immunohistochemical method. Western blot method was used to detect the expression of p-I 魏 B 偽 and p-NF- 魏 BP65 in hippocampal tissue. Results the expression of p-IKK 偽 / 尾 in the CA1 area of hippocampus of 0.25g / g group was 54.72 鹵10.04 鹵10.04 and 32.99 鹵5.42CA3, respectively. The expression of p-IKK 偽 / 尾 in the CA1 area of rats in the 0.2550 鹵4.92La Cl3 group was respectively 303.67 鹵34.786.83 鹵29.87.87.01 鹵40CA1CA3DG and 64.80 鹵8.2777.78 鹵4.92DG, respectively, compared with the control group, and the expression of IKK / 尾 in the hippocampus of the control group was higher than that of the control group, and the expression of p-IKK 偽 / 尾 in the hippocampus of the control group was significantly higher than that of the control group (P < 0.05), and the expression of p-IKK 偽 / 尾 in the hippocampal CA1 area of the rats in the group of 0.25 鹵0.567 鹵34.786.83 鹵29.87.01 鹵17.01 鹵17.01 鹵17.01 鹵17.01 鹵17.01 鹵17.01 鹵17.01, respectively. The expression of p-IKK 偽 / 尾 in hippocampus of rats in lanthanum group except DG area 0.25La Cl3 group was lower than that in control group (P 0.05 + 0.05) and 0.56 鹵0.056 鹵0.056 鹵0.05in La Cl3 group respectively. The expression of p-I 魏 B 偽 and p-NF- 魏 Bp65 in hippocampus were 0.58 鹵0.06, 0.46 鹵0.06 鹵0.07) and 0.36 鹵0.036 鹵0.036 鹵0.08 in La Cl3 group, respectively, which were lower than those in control group (0.68 鹵0.05) and 0.56 鹵0.08P0.05A, respectively. The expression of p-I 魏 B 偽 and p-NF#en4# 魏 魏 in hippocampus of neonatal rats were lower than those in control group (0.68 鹵0.05) and 0.56 鹵0.08P0.05A, respectively. There was a negative correlation between the expression of white and the dose of La Cl3. Conclusion the activity of NF- 魏 B in hippocampal cells of filial generation rats can be decreased by subchronic La C13 exposure. It may be one of the mechanisms of the decline of learning and memory induced by lanthanum.
【作者單位】： 遼東學(xué)院醫(yī)學(xué)院臨床Ⅱ教研室;中國醫(yī)科大學(xué)衛(wèi)生毒理教研室;
【基金】：國家自然科學(xué)基金(30901209) 遼寧省教育廳科研項目(L2010702) 遼東學(xué)院青年基金(2013q004)
【分類號】：R114

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 楊維東;張萍;劉潔生;莫伍興;;長期稀土釔暴露對大鼠學(xué)習(xí)記憶能力影響[J];中國公共衛(wèi)生;2008年02期

2 戚大煜;蔡原;巫生文;;哺乳期母鼠鑭暴露對子代腦組織氧化損傷影響[J];中國公共衛(wèi)生;2009年06期

【共引文獻(xiàn)】

相關(guān)期刊論文 前7條

1 夏青;劉會雪;楊曉達(dá);王夔;;稀土神經(jīng)毒性研究[J];中國科學(xué):化學(xué);2012年09期

2 戚大煜;蔡原;巫生文;;哺乳期母鼠鑭暴露對子代腦組織氧化損傷影響[J];中國公共衛(wèi)生;2009年06期

3 張t，

本文編號：1570933