本文關(guān)鍵詞： 生殖健康 多氯聯(lián)苯 快速溶劑萃取 氣相色譜質(zhì)譜　出處：《華南理工大學(xué)》2013年碩士論文　論文類型：學(xué)位論文

【摘要】：隨著工業(yè)生產(chǎn)的發(fā)展，環(huán)境污染引起的健康問題逐年加劇，其中對男性生殖健康的影響尤為突出。多氯聯(lián)苯是一類廣泛存在的環(huán)境有機(jī)污染物，具有不易降解、環(huán)境持久性、生物積聚性，對人體生殖健康存在潛在危害性。 本研究用快速溶劑萃�。ˋSE）法和氣相色譜質(zhì)譜（GC-MS）法測定血液及精液樣品中的6種指示性多氯聯(lián)苯（PCB52、PCB101、PCB118、PCB138、PCB153、PCB180）的濃度。旨在探討全血和精液中PCBs污染水平與男性生殖障礙之間的關(guān)系，建立簡便快捷的分析方法，為相關(guān)生殖健康研究提供數(shù)據(jù)依據(jù)，也可為我國的PCBs污染控制提供背景依據(jù)。 本研究建立了人體全血和精液ASE前處理方法及GC-MS的檢測PCBs的方法，實驗結(jié)果PCBs加標(biāo)回收率為：53-83%；各PCBs單體的方法檢出限為0.24-1.15ng/g。本方法符合分析全血和精液此類樣本的需要。 本研究通過不育門診就診的患者采集血液和精液，分析樣本中PCBs的濃度。發(fā)現(xiàn)血液中PCB52平均濃度為4.47ng/g，PCB101為2.70ng/g，PCB118未檢出，PCB138為8.14ng/g，PCB153為1.83ng/g，PCB180為10.24ng/g。精液樣本中PCB52濃度為3.84ng/g，PCB101為2.52ng/g，PCB118為0.45ng/g，PCB-138為4.42ng/g，PCB153為6.04ng/g，PCB180為6.86ng/g。結(jié)果表明，不育患者的血液和精液中的PCBs濃度遠(yuǎn)高于正常人平均水平。 通過不育患者與正常人血液及精液中PCBs濃度的對比發(fā)現(xiàn)，，不育患者血液及精液中的PCBs濃度較高。其中影響PCBs濃度的因素有：職業(yè)、居住地、BMI、年齡等。從事個體經(jīng)營的人血液及精液中的PCBs濃度較高，而IT行業(yè)的濃度較低；居住在市區(qū)的患者的精液中PCBs濃度比居住在郊區(qū)的患者PCBs濃度高，而血液則相反；BMI適中的人體內(nèi)PCBs濃度較低，過輕或過重都將導(dǎo)致PCBs濃度較高；研究還發(fā)現(xiàn)，實驗還發(fā)現(xiàn)患者的年齡越大血液和精液中總PCBs濃度越低。 目前，國內(nèi)外對PCBs與男性生殖健康的關(guān)系的研究還處于探索階段。本實驗為該方面的流行病學(xué)研究及環(huán)境激素所引起的生殖健康問題提供數(shù)據(jù)支持。
[Abstract]:With the development of industrial production, the health problems caused by environmental pollution are aggravated year by year, especially on male reproductive health. Polychlorinated biphenyls (PCBs) are a class of environmental organic pollutants, which are difficult to degrade and persistent in the environment. Biological accumulation has potential harm to human reproductive health. In this study, the rapid solvent extraction (ASE) method and gas chromatography-mass spectrometry (GC-MS) were used to determine the concentration of six indicative PCB52PCB101C PCB101P PCB118PIB138PIB153PCB180 in blood and semen samples. The aim of this study was to investigate the relationship between the levels of PCBs contamination in whole blood and semen and male reproductive disorders. To establish a simple and rapid analysis method, to provide data basis for related reproductive health research, but also to provide background basis for PCBs pollution control in China. In this study, the pretreatment method of ASE in human whole blood and semen and the method for detecting PCBs in human whole blood and semen were established. The results showed that the recovery rate of PCBs plus standard was 53-83.The detection limit of each PCBs monomer was 0.24-1.15ng / g. This method was suitable for the analysis of whole blood and semen samples. In this study, blood and semen were collected from infertile outpatients. Analysis of the concentration of PCBs in the sample. It was found that the average concentration of PCB52 in the blood was 4.47 ng / g / g PCB101 = 2.70 ng / g / g PCB118. No PCB138 was found to be 8.14 ng / g / g PCB153 = 1.83 ng / g / g / g PCB180 = 10.24 ng / g / g. The PCB52 concentration in semen samples was 3.84 ng / g / g / g PCB101 and 2.52 ng / g / g PCB118 was 0.45 ng / g / 138, 4.42 ng / g / g PCB153 was 6.04ng / g / g / 180 and 6.86 ng / g / 180, respectively. The results showed that. PCBs levels in the blood and semen of infertile patients were significantly higher than the normal average. By comparing the concentration of PCBs in blood and semen of infertile patients and normal people, it was found that the concentration of PCBs in blood and semen of infertile patients was higher. The factors influencing the concentration of PCBs were occupation. BMI, age, etc. The concentration of PCBs in blood and semen of self-employed people is higher than that in IT industry. The concentration of PCBs in semen of patients living in urban areas is higher than that of patients living in suburbs. On the other hand, people with moderate PCBs in the blood had lower PCBs concentration, too light or too heavy, which would lead to higher PCBs concentration. The study also found that the older the patient was, the lower the total PCBs concentration in blood and semen was. At present, the research on the relationship between PCBs and male reproductive health at home and abroad is still in the exploratory stage. This study provides data support for the epidemiological study in this field and reproductive health problems caused by environmental hormones.
【學(xué)位授予單位】：華南理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R114;X503.1

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