本文關鍵詞： 核桃內種皮 多酚 衰老 腦 氧化損傷　出處：《食品研究與開發(fā)》2017年11期 　論文類型：期刊論文

【摘要】：研究核桃內種皮多酚提取物對D-半乳糖致衰小鼠腦組織氧化損傷程度的調節(jié)。選取ICR雄性小鼠60只,隨機分為5組,每組12只,對模型對照組和劑量組小鼠采取頸部皮下注射D-半乳糖[120 mg/(kg·bw·d)]制造衰老模型,低、中、高劑量組分別灌胃200、400、800 mg/(kg·bw·d)的核桃內種皮多酚提取物,模型對照組灌胃等量的雙蒸水。給藥7周后,通過曠場試驗、緊繩實驗和平衡木實驗檢測每組小鼠間的行為學差異。8周后,取腦組織,測定腦組織勻漿中超氧化物歧化酶(SOD)、過氧化氫酶(CAT)、谷胱甘肽過氧化物酶(GSH-Px)活力和總抗氧化能力(T-AOC)及丙二醛(MDA)的含量。結果表明,與模型對照組相比,核桃內種皮多酚提取物可顯著提高小鼠的運動和探索能力,增強小鼠的興奮性和對環(huán)境的適應性。同時,腦組織中SOD、GSH-Px活力和T-AOC含量較模型組有顯著升高。這表明核桃內種皮多酚提取物可減輕D-半乳糖導致的行為遲鈍,并能減輕小鼠腦部的氧化損傷。
[Abstract]:To study the effect of polyphenol extract from inner seed coat of walnut on oxidative damage of brain tissue in aging mice induced by D-galactose, 60 male ICR mice were randomly divided into 5 groups, 12 rats in each group. The aging model was induced by subcutaneous injection of D-galactose (120 mg/(kg 路BW 路d) into the neck of mice in the model control group and the dose group. The low, middle and high dose groups were given 200,400,800 mg/(kg 路BW 路d of polyphenol extract from the inner coat of walnut respectively. The model control group was given the same amount of double-steamed water. After 7 weeks of administration, the behavioral differences between each group were detected by open-field test, tight-rope test and balance beam test. The activities of superoxide dismutase (SOD), catalase (cat), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) and malondialdehyde (MDA) in brain homogenate were determined. The polyphenol extract from the inner seed coat of walnut can significantly improve the exercise and exploration ability of mice, enhance the excitability and adaptability to the environment of the mice, at the same time, The activity of GSH-Px and the content of T-AOC in brain tissue were significantly higher than those in the model group, which indicated that the polyphenol extract from walnut seed coat could alleviate the behavioral retardation induced by D-galactose and reduce the oxidative damage to the brain of mice.
【作者單位】： 食品營養(yǎng)與安全省部共建教育部重點實驗室食品工程與生物技術學院天津科技大學;天津科技大學新農村發(fā)展研究院;
【基金】：天津科技大學新農村發(fā)展研究院開放基金資助項目(XNC201511) 教育部科技發(fā)展中心“藍火計劃”產學研聯(lián)合創(chuàng)新項目衡水專項2014年度項目(2014-LHJH-HSZX-017)
【分類號】：R151
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本文編號：1553318