本文關(guān)鍵詞： 砷化合物形態(tài) 非致瘤性人源性肝細(xì)胞 細(xì)胞凋亡　出處：《環(huán)境與健康雜志》2017年01期 　論文類型：期刊論文

【摘要】：目的探討不同形態(tài)砷化合物對非致瘤性人源性肝(QSG7701)細(xì)胞的毒性及氧化應(yīng)激作用。方法將處于對數(shù)生長期的QSG7701細(xì)胞分別暴露于亞砷酸鈉(砷濃度為1、5、25、100μmol/L)、砷酸鈉(砷濃度為10、25、100、500μmol/L)及MMA和DMA(砷濃度均為100、500、1 000、2 000μmol/L)培養(yǎng)24 h,并設(shè)對照(含10%胎牛血清的RPMI-1640培養(yǎng)基)。采用四甲基偶氮唑鹽(MTT)法測定細(xì)胞活性;檢測細(xì)胞培養(yǎng)液中乳酸脫氫酶(LDH)釋放率、谷草轉(zhuǎn)氨酶(AST)活力、總超氧化物歧化酶(T-SOD)活力和丙二醛(MDA)含量。結(jié)果一定濃度的As~(Ⅲ)(≥1μmol/L)、As~Ⅴ(≥10μmol/L)、MMA(≥100μmol/L)和DMA(≥2 000μmol/L)均能顯著降低QSG7701細(xì)胞的存活率,差異有統(tǒng)計學(xué)意義(P0.05);且隨著As~(Ⅲ)、As~Ⅴ及MMA和DMA染毒濃度的升高,QSG7701細(xì)胞的存活率呈逐漸降低的趨勢。經(jīng)計算,QSG7701細(xì)胞暴露于As~(Ⅲ)、As~Ⅴ、MMA和DMA 24 h的IC_(50)分別為170.89、863.73、2 235.67、4 045.31μmol/L,對QSG7701細(xì)胞生長的抑制作用依次為As~(Ⅲ)As~ⅤMMADMA。在當(dāng)As~(Ⅲ)濃度為5~100μmol/L、As~Ⅴ濃度為10~500μmol/L及MMA中的砷濃度為1 000、2 000μmol/L和DMA中的砷濃度為2 000μmol/L時,QSG7701細(xì)胞的LDH釋放率均高于對照組,差異有統(tǒng)計學(xué)意義(P0.05);另外,當(dāng)As~(Ⅲ)濃度為1~100μmol/L、As~Ⅴ濃度為10~500μmol/L及MMA和DMA中的砷濃度為100~2 000μmol/L時,QSG7701細(xì)胞培養(yǎng)液中的AST活力均高于對照組;且隨著As~(Ⅲ)、As~Ⅴ及MMA和DMA染毒濃度的升高,QSG7701細(xì)胞的LDH釋放率和細(xì)胞培養(yǎng)液中的AST活力呈上升的趨勢。與對照組比較,當(dāng)As~(Ⅲ)濃度為5~100μmol/L、As~Ⅴ濃度為10~500μmol/L時,QSG7701細(xì)胞中的MDA含量均較高,而T-SOD活力均較低;與對照組比較,當(dāng)MMA和DMA中的砷濃度均為500~2 000μmol/L時,QSG7701細(xì)胞中的MDA含量均較高;而當(dāng)MMA中的砷濃度為100~2 000μmol/L和DMA中的砷濃度為100、500μmol/L時,QSG7701細(xì)胞中的T-SOD活力均較低,差異有統(tǒng)計學(xué)意義(P0.05)。結(jié)論在本實驗條件下,4種砷化合物均可不同程度地?fù)p傷肝細(xì)胞膜,破壞肝細(xì)胞的氧化平衡狀態(tài),產(chǎn)生氧化應(yīng)激并導(dǎo)致脂質(zhì)過氧化,對人肝細(xì)胞QSG7701的毒性作用依次為As~(Ⅲ)As~ⅤMMADMA。
[Abstract]:Objective to investigate the toxicity and oxidative stress of different arsenic compounds on non-tumorigenic human hepatocytes QSG7701. Methods the QSG7701 cells in logarithmic growth phase were exposed to sodium arsenite (arsenic concentration of 1 5 ~ 25 渭 mol 路L ~ (-1)) and sodium arsenate (arsenic concentration) to sodium arsenite (arsenic concentration). MMA and DMA (100 渭 mol / L, 100 渭 mol / L) were cultured for 24 h, and the control medium (RPMI-1640 medium containing 10% fetal bovine serum) was used to determine the cell activity by using tetramethyl azolium salt (MTT) method. The concentration of arsenic was 100 渭 mol 路L ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ 1 000 渭 mol 路L ~ (-1) 路L ~ (-1) ~ (-1) ~ (-1). The release rate of lactate dehydrogenase (LDH) and the activity of glutamic oxaloacetic transaminase (AST) in cell culture medium were measured. The activity of total superoxide dismutase (T-SOD) and the content of malondialdehyde (MDA) were determined. Results the survival rate of QSG7701 cells was significantly decreased at a certain concentration (鈮，

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