本文關(guān)鍵詞： PM2.5 TNF-α IL-1 IL-2 IL-4 鈣調(diào)磷酸酶(CaN) 白介素-1受體相關(guān)激酶-1(IRAK-1) 活化T細(xì)胞核因子(NFAT)　出處：《山西醫(yī)科大學(xué)》2013年碩士論文　論文類型：學(xué)位論文

【摘要】：目的： 探討太原市交通相關(guān)PM2.5對人群外周血細(xì)胞因子的影響,并初步探討交通相關(guān)PM2.5通過外周血淋巴細(xì)胞Ca2+-CaN/IRAK-1—NFAT信號通路調(diào)控細(xì)胞因子,對深入探討PM2.5的免疫毒性機(jī)制提供參考依據(jù)。 方法： 選擇太原市外勤交通警察為目標(biāo)人群,疾病預(yù)防控制中心(CDC)工作人員為對照人群,監(jiān)測兩人群工作環(huán)境中PM2.5濃度,采集并分離兩人群外周血淋巴細(xì)胞,采用熒光分光光度法檢測不同人群淋巴細(xì)胞內(nèi)[Ca2+]i的變化；ELISA法檢測人群血清中細(xì)胞因子TNF-α、IL-1、IL-2、IL-4的表達(dá)水平；熒光定量PCR(Q-PCR)法測定細(xì)胞鈣調(diào)磷酸酶(CaN)、白介素-1受體相關(guān)激酶-1(IRAK-1)、活化T細(xì)胞核因子(NFAT)、IL-2和TNF-α基因的mRNA表達(dá)；Western Blotting測定CaN、IRAK-1、NFAT蛋白表達(dá)情況。 結(jié)果： 太原市交警工作環(huán)境中PM2.5濃度(0.132mg/m3)高于CDC人員工作環(huán)境中PM2.5濃度(0.100mg/m3)(P0.05)。交警淋巴細(xì)胞內(nèi)鈣離子濃度高于CDC人員。交警淋巴細(xì)胞內(nèi)IL-2mRNA表達(dá)比CDC工作人員低(P0.05)；男性交警TNF-α mRNA表達(dá)水平比CDC工作人員高(P0.05)。交警血清中細(xì)胞因子TNF-α、 IL-1表達(dá)水平比CDC工作人員高(P0.05)；而交警血清IL-2表達(dá)水平比CDC工作人員低(P0.05)；交警淋巴細(xì)胞內(nèi)CaN mRNA和IRAK-1mRNA表達(dá)水平比CDC工作人員有增高趨勢,交警淋巴細(xì)胞內(nèi)NFAT mRNA表達(dá)水平比CDC工作人員高(P0.05)。血清濃度與淋巴細(xì)胞內(nèi)Ca2+濃度、血清TNF-α濃度與CaN和NFAT基因表達(dá)均有正相關(guān)關(guān)系(P0.05),血清IL-2水平與Ca2+呈負(fù)相關(guān)關(guān)系(P0.05)。 結(jié)論： 太原市交通相關(guān)PM2.5可增加外周血IL-1和TNF-α細(xì)胞因子表達(dá),抑制IL-2分泌,外周血淋巴細(xì)胞Ca2+-CaN/IRAK-1-NFAT信號通路可能參與TNF-α的調(diào)控。
[Abstract]:Objective:. To investigate the effect of traffic related PM2.5 on peripheral blood cytokines in Taiyuan, and to explore the regulation of cytokines by traffic related PM2.5 through the Ca2 -CaN / IRAK-1-NFAT signaling pathway in peripheral blood lymphocytes, which provides a reference for the further study of the immunotoxicity mechanism of PM2.5. Methods:. The field traffic police in Taiyuan city were selected as the target population and the staff of the CDC as the control group. The concentration of PM2.5 in the working environment of the two populations was monitored and the peripheral blood lymphocytes were collected and separated from the two populations. The changes of [Ca2] I in lymphocytes of different populations were detected by fluorescence spectrophotometry and the expression level of cytokine TNF- 偽, IL-1and IL-2IL-4 in serum of different populations was detected by enzyme-linked immunosorbent assay (Elisa). The expression of calcium phosphatase (Cam), interleukin-1 receptor associated kinase (IL-1) and IRAK-1was detected by fluorescence quantitative PCR- Q-PCR method. The mRNA expression of IL-2 and TNF- 偽 gene was activated by T cell nuclear factor, and the expression of CaNNRAK-1nFAT protein was detected by Western Blotting. Results:. The concentration of PM2.5 in the working environment of traffic police in Taiyuan City was 0.132 mg / m ~ (3) higher than that in the working environment of CDC personnel (0.100 mg / m ~ (3)) and the concentration of Ca ~ (2 +) in the lymphocytes of traffic police was higher than that of CDC personnel. The expression of IL-2mRNA in the lymphocytes of traffic police was lower than that of CDC workers (P 0.05); the concentration of IL-2mRNA in the lymphocytes of male traffic police was lower than that of CDC workers. The expression level of mRNA was higher than that of CDC staff. The expression level of cytokine TNF- 偽 and IL-1 was higher than that of CDC staff, while the level of IL-2 in traffic police was lower than that of CDC staff. The expression level of CaN mRNA and IRAK-1mRNA in the lymphocytes of traffic police was higher than that of CDC staff. There is a tendency for CDC staff to increase, The expression level of NFAT mRNA in the lymphocytes of traffic police was higher than that of the CDC workers. There was a positive correlation between the serum concentration of TNF- 偽 and the expression of CaN and NFAT gene, and a negative correlation between the level of serum IL-2 and Ca2. Conclusion:. Traffic related PM2.5 in Taiyuan can increase the expression of IL-1 and TNF- 偽 cytokines and inhibit the secretion of IL-2. The Ca2 -CaN / IRAK-1-NFAT signaling pathway in peripheral blood lymphocytes may be involved in the regulation of TNF- 偽.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R128

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