本文關(guān)鍵詞： 氟化鈉 雌性生殖功能 生殖激素 受體 大鼠　出處：《重慶醫(yī)科大學(xué)》2013年碩士論文　論文類型：學(xué)位論文

【摘要】：目的 氟化鈉對人類生殖功能的影響，尤其是對雌性生殖的影響是越來越受到研究者們的關(guān)注。然而有關(guān)氟化鈉對雌性生殖影響的研究報道很是少見，影響的機制尚不清楚。本研究旨在探索氟化鈉對雌性生殖的影響極其相關(guān)機制。 方法 構(gòu)建SD大鼠氟化鈉中毒模型。將SD雌性大鼠隨機分為三組，即對照組，100mg/L組和200mg/L組；分別飲用蒸餾水，濃度為100mg/L和200mg/L的氟化鈉溶液，染毒180天。染毒期結(jié)束后各組雌性大鼠分別與雄性大鼠交配觀察其妊娠率變化。采用ELISA法檢測氟化鈉染毒大鼠血清生殖激素濃度(E2,P, FSH, LH和T)的改變。用H-E染色的方法觀察各組雌性大鼠子宮組織形態(tài)學(xué)變化。采用免疫組織化的方法對子宮雌激素α受體和孕激素受體進行組織學(xué)定位。利用Western blot方法檢測雌激素α受體和孕激素受體在不同濃度的氟化鈉染毒SD雌性大鼠子宮中的表達情況。 結(jié)果 1、氟化鈉染毒組大鼠妊娠率顯著降低。 2、血清中E2、P、FSH、LH和T濃度分別顯著低于對照組，染毒組雌性大鼠子宮顯微結(jié)構(gòu)發(fā)生了明顯異常改變。 3、氟化鈉暴露組大鼠子宮雌激素α受體和孕激素受體表達較對照組明顯上調(diào)。 結(jié)論 氟化鈉對雌性生殖功能造成了顯著影響，，降低了雌性大鼠妊娠率。生殖激素的降低，雌激素受體及孕激素受體蛋白的異常表達可能是氟化鈉對雌性生殖功能影響的機制之一。
[Abstract]:Purpose. The effects of sodium fluoride on human reproductive function, especially on female reproduction, have attracted increasing attention from researchers. However, studies on the effects of sodium fluoride on female reproduction are rare. The effect of sodium fluoride on female reproduction is not clear. Method. The SD female rats were randomly divided into three groups: the control group 100 mg / L and the 200 mg / L group, drinking distilled water, 100 mg / L and 200 mg / L sodium fluoride solution, respectively. At the end of the exposure period, female rats in each group were mated with male rats to observe their pregnancy rate. Serum reproductive hormone concentrations of E2P, FSH, LH and T4 were detected by ELISA method. Methods the morphologic changes of uterus in each group of female rats were observed. The histological localization of estrogen 偽 receptor and progesterone receptor in uterus was performed by immunohistochemical method. The estrogen 偽 receptor and pregnancy excitation were detected by Western blot method. Expression of hormone receptor in uterus of SD female rats exposed to different concentrations of sodium fluoride. Results. 1. The pregnancy rate of rats exposed to sodium fluoride decreased significantly. (2) the serum levels of FSHLH LH and T in serum were significantly lower than those in the control group, and the microstructures of the uterus in the exposed group were significantly different from those in the control group. 3. The expression of estrogen 偽 receptor and progesterone receptor in uterus of rats exposed to sodium fluoride was significantly higher than that of control group. Conclusion. Sodium fluoride had a significant effect on female reproductive function and decreased the pregnancy rate of female rats. The abnormal expression of estrogen receptor and progesterone receptor protein may be one of the mechanisms of sodium fluoride affecting female reproductive function.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R114

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