本文關(guān)鍵詞： 赭曲霉毒素A MTT ROS 彗星試驗(yàn) DNA損傷　出處：《甘肅農(nóng)業(yè)大學(xué)學(xué)報(bào)》2016年01期 　論文類(lèi)型：期刊論文

【摘要】：【目的】研究不同濃度和時(shí)間處理下,赭曲霉毒素A(OTA)對(duì)人結(jié)腸癌細(xì)胞(Caco-2)的細(xì)胞生長(zhǎng)抑制、氧化損傷以及DNA損傷的影響.【方法】采用0.32、1.6、8、40、200μmol/L OTA處理Caco-2細(xì)胞24、48、72h后,利用甲基偶氮唑鹽(MTT)法檢測(cè)細(xì)胞存活率,利用活性氧(ROS)試劑盒檢測(cè)細(xì)胞內(nèi)的自由基水平,利用彗星試驗(yàn)檢測(cè)細(xì)胞的DNA損傷.【結(jié)果】在各個(gè)處理時(shí)間段內(nèi),OTA濃度大于0.32μmol/L時(shí),OTA對(duì)細(xì)胞存活率都有顯著的抑制作用(P0.05);細(xì)胞內(nèi)ROS以及DNA損傷都隨OTA濃度的增加而顯著升高(P0.05),尤其在0~8μmol/L低濃度范圍內(nèi),OTA顯示了強(qiáng)烈的毒性.【結(jié)論】OTA可導(dǎo)致Caco-2細(xì)胞存活率降低,引起細(xì)胞氧化應(yīng)激以及DNA損傷,其中ROS水平升高可能是OTA導(dǎo)致Caco-2細(xì)胞DNA損傷的原因.
[Abstract]:[Objective] to study the effects of different concentrations and time of treatment, ochratoxin A (OTA) on human colon cancer cells (Caco-2) cell growth inhibition effect of oxidative damage and DNA damage. [method] by 0.32,1.6,8,40200 mol/L OTA in Caco-2 cells treated with 24,48,72h, using methyl thiazolyl tetrazolium (MTT) survival rate method detection of cell, using active oxygen free radical level (ROS) kit for detection of intracellular DNA damage, using comet assay cells. [results] in each treatment time, OTA concentration is greater than 0.32 mol/L, OTA ratio has significant inhibitory effect on the cell (P0.05) cells; ROS and DNA damage increased with the increase of OTA concentration increased significantly (P0.05), especially in the 0~8 mol/L range of low concentration, OTA showed strong toxicity. [Conclusion] OTA can induce Caco-2 cell survival rate decreased, caused by oxidative stress and DNA damage The elevated level of ROS may be the cause of the DNA damage to Caco-2 cells by OTA.

【作者單位】： 甘肅農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)技術(shù)學(xué)院;中國(guó)農(nóng)業(yè)科學(xué)院北京畜牧獸醫(yī)研究所農(nóng)業(yè)部奶產(chǎn)品質(zhì)量安全風(fēng)險(xiǎn)評(píng)估實(shí)驗(yàn)室(北京);中國(guó)農(nóng)業(yè)科學(xué)院北京畜牧獸醫(yī)研究所動(dòng)物營(yíng)養(yǎng)學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室;唐山市畜牧水產(chǎn)品質(zhì)量監(jiān)測(cè)中心;
【基金】：公益性行業(yè)(農(nóng)業(yè))科研專(zhuān)項(xiàng)項(xiàng)目“生鮮乳質(zhì)量安全評(píng)價(jià)技術(shù)與生產(chǎn)規(guī)程”(201403071) 生鮮乳質(zhì)量安全風(fēng)險(xiǎn)評(píng)估專(zhuān)項(xiàng)(2014-z3) 中國(guó)農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程(ASTIP-IAS12)
【分類(lèi)號(hào)】：R114
【正文快照】： 3.中國(guó)農(nóng)業(yè)科學(xué)院北京畜牧獸醫(yī)研究所,農(nóng)業(yè)部奶產(chǎn)品質(zhì)量安全風(fēng)險(xiǎn)評(píng)估實(shí)驗(yàn)室(北京),北京100193;4.中國(guó)農(nóng)業(yè)科學(xué)院北京畜牧獸醫(yī)研究所動(dòng)物營(yíng)養(yǎng)學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室,北京100193)赭曲霉毒素A(OTA)主要是由純綠青霉,赭曲霉和炭黑曲霉產(chǎn)生的真菌毒素,廣泛存在于自然界,能污染多種植物產(chǎn)，

本文編號(hào)：1485956