發(fā)布時間：2018-01-31 14:07

  本文關(guān)鍵詞： 交通污染 機動車尾氣顆粒物 病理組織學(xué) 單細(xì)胞凝膠電泳 細(xì)胞色素P450側(cè)鏈裂解酶 實時熒光定量RT-PCR　出處：《福建醫(yī)科大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：本研究通過建立現(xiàn)場暴露交通污染環(huán)境和腹腔注射機動車尾氣顆粒物兩種染毒試驗，觀察和分析交通性污染對雄性小鼠生殖毒性作用及可能的分子機制，為人群生育健康的促進(jìn)和制定有效保護(hù)措施提供科學(xué)依據(jù)。 方法：本研究通過建立現(xiàn)場暴露交通污染環(huán)境染毒試驗（將30只清潔級雄性昆明系小鼠隨機分為重度污染暴露組、輕度污染暴露組和清潔環(huán)境空白對照組3個組，每組10只，進(jìn)行現(xiàn)場暴露染毒，每日持續(xù)暴露10h，持續(xù)暴露35d。）的基礎(chǔ)上，結(jié)合腹腔注射機動車尾氣顆粒物染毒試驗（將40只清潔級ICR雄性小鼠隨機分為對照組（PBS溶液）、低劑量染毒組(0.4mg/ml)、中劑量染毒組(2mg/ml)和高劑量染毒組(10mg/ml)4個組，每組10只，每只小鼠均按10ml/kg的注射量進(jìn)行，隔24h注射一次，持續(xù)35d）。染毒后觀察和分析交通性污染對各組實驗小鼠生殖器官發(fā)育、精子數(shù)量和質(zhì)量、睪丸和附睪組織病理學(xué)變化等可能存在的生殖毒性并通過分析實驗小鼠睪丸細(xì)胞DNA損傷情況和血清睪酮含量以及與其分泌密切相關(guān)基因P450scc和StAR的mRNA的相對表達(dá)量探討可能的分子機制。 結(jié)果：現(xiàn)場暴露交通污染環(huán)境染毒試驗和腹腔注射機動車尾氣顆粒物染毒試驗均發(fā)現(xiàn)交通性污染物能影響實驗小鼠生殖器官的發(fā)育，現(xiàn)場暴露交通污染環(huán)境染毒試驗的臟器系數(shù)分析顯示與對照組小鼠相比，重度暴露組小鼠體重、睪丸和附睪重、睪丸臟器系數(shù)下降；腹腔注射機動車尾氣顆粒物染毒試驗顯示高劑量組的附睪質(zhì)量、附睪臟器系數(shù)下降，差異有統(tǒng)計學(xué)意義(P＜0.05)。組織病理學(xué)結(jié)果顯示：現(xiàn)場暴露交通污染環(huán)境染毒試驗中輕度和重度暴露組以及腹腔注射機動車尾氣顆粒物染毒試驗中中劑量和高劑量小鼠睪丸和附睪組織有不同程度損傷。與對照組相比，輕度和重度暴露組以及中劑量和高劑量小鼠精子數(shù)量下降，精子畸形率上升，經(jīng)檢驗以上差異均具有統(tǒng)計學(xué)意義（P0.05）；在分子機制研究中彗星試驗顯示：重度暴露組小鼠睪丸細(xì)胞DNA損傷率增加，與空白對照組相比，差異具有統(tǒng)計學(xué)意義（P0.05）；腹腔注射機動車尾氣顆粒物染毒試驗顯示高劑量組血清睪酮含量以及睪丸組織基因P450scc mRNA相對表達(dá)量，，與對照組相比下降,差異具有統(tǒng)計學(xué)意義(P＜0.05)。 結(jié)論：交通污染以及交通污染重要組成成分機動車尾氣顆粒物可能通過直接損睪丸細(xì)胞DNA和（或）影響睪丸的分泌以及與其分泌密切相關(guān)的基因P450sccmRNA的相對表達(dá)量的作用機制對雄性小鼠生殖系統(tǒng)的發(fā)育和功能造成一定程度的損傷。
[Abstract]:Objective: to observe and analyze the effect of traffic pollution on male mice reproductive toxicity and its possible molecular mechanism by setting up two kinds of exposure tests: field exposure to traffic pollution environment and intraperitoneal injection of motor vehicle exhaust particulates to observe and analyze the effects of traffic pollution on male mice reproductive toxicity. To provide scientific basis for the promotion of population reproductive health and the formulation of effective protection measures. Methods: in this study, 30 clean grade male Kunming mice were randomly divided into severe pollution exposure group by setting up field exposure test. Light pollution exposure group and clean environment blank control group 3 groups, each group 10 rats, field exposure exposure, 10 hours a day, continuous exposure 35 d.on the basis of. Combined with intraperitoneal injection of motor vehicle exhaust particles (40 clean grade ICR male mice were randomly divided into control group, low dose group, 0. 4 mg / ml). The middle dose group (2 mg / ml) and the high dose group (10 mg / ml), 10 mice in each group were injected at 10 ml / kg, once every 24 hours. After 35 days of exposure, the development of reproductive organs, the quantity and quality of spermatozoa were observed and analyzed. The possible reproductive toxicity of testicular and epididymal histopathological changes was analyzed by analyzing the DNA damage in testicular cells, serum testosterone content and the genes P450 SCC and S closely related to the secretion of testicular cells in experimental mice. The molecular mechanism of the relative expression of mRNA in tAR was studied. Results: it was found that traffic pollutants could affect the development of reproductive organs of experimental mice in field exposure to traffic pollution environment and intraperitoneal injection of motor vehicle exhaust particulates. Compared with the control group, the body weight, testis and epididymis weight and testis organ coefficient in the severe exposure group were lower than those in the control group. The mass of epididymis and organ coefficient of epididymis were decreased in high dose group. The difference was statistically significant (P < 0.05). ... histopathological results show that:. The testis and epididymis tissues of mice exposed to traffic pollution in the mild and severe exposure group and the intraperitoneal injection of motor vehicle exhaust particulates were damaged in varying degrees, as compared with those in the control group. Compared to. In mild and severe exposure groups, the number of sperm decreased and the rate of sperm malformation increased in moderate and high dose mice. The above differences were statistically significant (P 0.05). In the study of molecular mechanism, comet assay showed that the DNA damage rate of testicular cells in the severe exposure group was increased, compared with the control group, the difference was statistically significant (P 0.05). The experimental results of intraperitoneal injection of motor vehicle exhaust particles showed that the serum testosterone content and the relative expression of P450 SCC mRNA in testis tissue in high dose group were lower than those in control group. The difference was statistically significant (P < 0.05). Conclusion: traffic pollution and traffic pollution are important components of vehicle exhaust particles, which may directly damage testicular cells DNA and (or). The mechanism of affecting the secretion of testis and the relative expression of P450scc mRNA, which is closely related to the secretion of testis, caused some damage to the development and function of the reproductive system of male mice.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R114

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 趙榮坡,楊石強,黃鸝,常笑雪,張鑫,徐正順,劉潤芳;司機職業(yè)不育男性的精液分析[J];中華男科學(xué);2003年07期

2 袁東,周偉,葉舜華,傅崇影;無鉛汽油推廣二年來公交司售人員健康狀況調(diào)查[J];勞動醫(yī)學(xué);2000年01期

3 張文麗,徐東群,崔九思;空氣細(xì)顆粒物(PM_(2.5))污染特征及其毒性機制的研究進(jìn)展[J];中國環(huán)境監(jiān)測;2002年01期

4 范軼歐,金一和,麻懿馨,張穎花,張曉們,齊藤憲光;全氟辛烷磺酸對雄性大鼠生精功能的影響[J];衛(wèi)生研究;2005年01期

5 黃勵,胡衡生,陳學(xué)斌;南寧市道路空氣污染對交通警察呼吸系統(tǒng)的影響[J];環(huán)境與健康雜志;2005年04期

6 彭寶成，葉舜華;汽油車尾氣排出物的致突變和致癌效應(yīng)[J];國外醫(yī)學(xué).衛(wèi)生學(xué)分冊;1995年03期

7 原福勝,馬亞萍,趙五紅;不同粒徑顆粒物對人雙核淋巴細(xì)胞微核率的影響[J];衛(wèi)生毒理學(xué)雜志;1999年02期

8 張遵真,梁英,冉云,茍小靜,林川,蔡春華,吳德生;汽油與甲醇燃燒汽車尾氣致突變性的對比研究[J];衛(wèi)生研究;2005年02期

9 錢曉薇;交通性污染物致小鼠突變性的研究[J];江西科學(xué);2004年01期

10 朱文兵,范立青,曾亞榮,盧光t;巴氏染色法在精子頂體研究中的應(yīng)用[J];中國現(xiàn)代醫(yī)學(xué)雜志;2005年03期

本文編號：1479239