本文關(guān)鍵詞：傳感技術(shù)在食品安全和環(huán)境監(jiān)測(cè)中的應(yīng)用研究　出處：《華中科技大學(xué)》2013年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 電化學(xué)傳感器 酶熱生物分析儀 多壁碳納米管 鹽酸萊克多巴胺 化學(xué)需氧量

【摘要】：萊克多巴胺屬β2-腎上腺素受體激動(dòng)劑，它能夠促進(jìn)動(dòng)物生長(zhǎng)，減少動(dòng)物體脂肪沉積，，提高蛋白質(zhì)含量，增加瘦肉率，屬于廣義的“瘦肉精”�；瘜W(xué)需氧量是間接反映水質(zhì)有機(jī)污染程度的綜合指標(biāo)，是水質(zhì)常規(guī)監(jiān)測(cè)的項(xiàng)目之一。及時(shí)了解和掌控化學(xué)需氧量，對(duì)環(huán)境水質(zhì)的評(píng)價(jià)和廢水的治理具有非常重要的意義。本論文利用電化學(xué)傳感器和酶熱生物傳感器，致力于鹽酸萊克多巴胺和化學(xué)需氧量的傳感器檢測(cè)新方法研究。 本研究主要由以下兩部分組成 第一部分多壁碳納米管修飾電極測(cè)定鹽酸萊克多巴胺的研究目的：建立一種高靈敏度、快捷簡(jiǎn)便的測(cè)定鹽酸萊克多巴胺的分析方法。方法：以鹽酸萊克多巴胺為研究對(duì)象，利用多壁碳納米管（MWNT）的優(yōu)異性能，以雙十六烷基磷酸酯（DHP）為分散劑，制備出化學(xué)修飾電極，系統(tǒng)地研究了鹽酸萊克多巴胺在玻碳電極和MWNT/DHP復(fù)合膜修飾電極上的電化學(xué)行為�？疾炝酥С蛛娊赓|(zhì)pH值、MWNT修飾量、富集時(shí)間及富集電位對(duì)氧化信號(hào)的影響，得到了最優(yōu)實(shí)驗(yàn)條件。 結(jié)果：該方法線性范圍為50μg/L~2mg/L，檢出限為20μg/L，平行10次的RSD為5.5%。最終將此方法用在豬肝和豬肉樣品中對(duì)鹽酸萊克多巴胺的實(shí)際檢測(cè)，回收率為93.1~107.2%，結(jié)果令人滿意。 結(jié)論：MWNT修飾電極能顯著提高鹽酸萊克多巴胺的電化學(xué)響應(yīng)信號(hào)，該方法有較高的準(zhǔn)確性。 第二部分酶熱生物分析儀測(cè)定水體化學(xué)需氧量 目的：利用酶熱生物分析儀結(jié)合流動(dòng)注射分析技術(shù)建立測(cè)定地表水化學(xué)需氧量（COD）的新方法。 方法：通過(guò)熱傳導(dǎo)器檢測(cè)來(lái)自次氯酸鈉與水體中有機(jī)污染物反應(yīng)產(chǎn)生的熱量來(lái)間接測(cè)定COD。以甘氨酸作為測(cè)定標(biāo)準(zhǔn)物，次氯酸鈉為氧化劑，探究了酶熱生物分析儀測(cè)定COD的機(jī)理并研究了最佳反應(yīng)條件。 結(jié)果：借助酶熱生物分析儀實(shí)現(xiàn)了對(duì)化學(xué)需氧量的快速測(cè)定，分析時(shí)間僅需5~7min（標(biāo)準(zhǔn)方法需2h），線性范圍為5~1000mg/L，檢出限為0.74mg/L。另外，該方法對(duì)氯離子有較高耐受性，對(duì)于10mg/L COD標(biāo)準(zhǔn)溶液，0.015M的氯離子對(duì)COD測(cè)定沒(méi)有干擾。在進(jìn)行實(shí)際水的檢測(cè)時(shí)，得到的結(jié)果與COD標(biāo)準(zhǔn)測(cè)定方法的相關(guān)性較好。 結(jié)論：用酶熱生物分析儀測(cè)定化學(xué)需氧量具有可行性。
[Abstract]:Ractopamine is a 尾 2-adrenergic agonist that promotes animal growth, reduces fat deposition in animals, increases protein content, and increases lean meat rates. Chemical oxygen demand (COD) is a comprehensive index that indirectly reflects the degree of organic pollution of water quality and is one of the items of routine monitoring of water quality. The evaluation of environmental water quality and the treatment of wastewater are of great significance. In this paper, electrochemical sensors and enzyme thermal biosensors are used. A new method of sensor detection for ractopamine hydrochloride and chemical oxygen demand (COD) has been developed. This study is mainly composed of the following two parts Part I study on determination of ractopamine hydrochloride with multi-walled carbon nanotube modified electrode objective: to establish a new method for the determination of ractopamine hydrochloride with high sensitivity. Methods: taking ractopamine hydrochloride as the object of study, the excellent performance of MWNTs was obtained by using multiwalled carbon nanotubes (MWNTs). The chemically modified electrode was prepared by using DHPas as dispersant. The electrochemical behavior of ractopamine hydrochloride on glassy carbon electrode and MWNT/DHP composite membrane modified electrode was studied systematically. The effects of enrichment time and potential on the oxidation signal were investigated and the optimal experimental conditions were obtained. Results: the linear range of the method was 50 渭 g / L and the detection limit was 20 渭 g / L. The RSD of parallel 10 times was 5.5. Finally, the method was applied to the determination of ractopamine hydrochloride in pig liver and pork samples. The recovery of ractopamine hydrochloride was 93.1% 107.2, and the results were satisfactory. Conclusion the electrochemical response of ractopamine hydrochloride can be significantly improved by the modified electrode, and the method has high accuracy. Part II determination of Chemical oxygen demand in Water by enzyme Thermobiological Analyzer Objective: to establish a new method for the determination of chemical oxygen demand (COD) in surface water by enzyme thermobiological analyzer and flow injection analysis. Methods: the heat produced from the reaction of sodium hypochlorite with organic pollutants in water was detected indirectly by means of heat transducers. Glycine was used as standard material and sodium hypochlorite as oxidant. The mechanism of COD determination by enzyme thermobiological analyzer was investigated and the optimum reaction conditions were studied. Results: the rapid determination of chemical oxygen demand was realized by enzyme thermobiological analyzer. The analytical time was only 5 ~ 7 min (the standard method needed 2 h), and the linear range was 5 ~ 1 000 mg / L. The detection limit is 0.74 mg / L. In addition, the method has high tolerance to chloride ions, and is suitable for 10 mg / L COD standard solution. The chlorine ion of 0.015m has no interference with the determination of COD, and the results obtained in the detection of real water have a good correlation with the standard method of COD determination. Conclusion: it is feasible to determine COD by enzyme thermobiological analyzer.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R155.5

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