本文關(guān)鍵詞：SPE-HPLC法檢測(cè)早勝牛肉中土霉素、四環(huán)素和金霉素的殘留　出處：《國(guó)際藥學(xué)研究雜志》2016年02期 　論文類型：期刊論文

  更多相關(guān)文章： 固相萃取-高效液相色譜法 早勝牛肉 土霉素 四環(huán)素 金霉素

【摘要】：目的建立同時(shí)測(cè)定早勝牛肉中殘留的土霉素、四環(huán)素和金霉素固相萃取-高效液相色譜方法。方法以Na2EDTA-Mcllvaine緩沖溶液(p H4.0)為樣品提取液,15%三氯乙酸為蛋白沉淀劑,固相萃取柱凈化富集目標(biāo)物,草酸0.01mol/L/乙腈/甲醇溶液為流動(dòng)相,紫外檢測(cè)器350 nm處檢測(cè),外標(biāo)法進(jìn)行定量。結(jié)果在優(yōu)化前處理和分析條件下,3種抗生素在質(zhì)量濃度范圍內(nèi)線性關(guān)系良好,相關(guān)系數(shù)r20.998,土霉素、四環(huán)素、金霉素的檢出限分別為18、15和30μg/kg,樣品加標(biāo)平均回收率為76.10%~95.22%,相對(duì)標(biāo)準(zhǔn)偏差10%。結(jié)論方法的靈敏度和精密度較高,且重復(fù)性好,適用于牛肉樣品中土霉素、四環(huán)素和金霉素殘留的檢測(cè)。
[Abstract]:Objective to establish a simultaneous determination of oxytetracycline in Zaosheng beef. Tetracycline and chlortetracycline were extracted by high performance liquid chromatography (HPLC). Methods the Na2EDTA-Mcllvaine buffer solution was used as the sample extraction solution. 15% trichloroacetic acid was used as protein precipitating agent, solid phase extraction column was used to purify and enrich the target, oxalic acid 0.01mol / L / acetonitrile / methanol solution was used as mobile phase, and UV detector was 350nm. Results under the optimized conditions of pretreatment and analysis, there was a good linear relationship between the three antibiotics in the range of mass concentration, the correlation coefficient was r20.998, oxytetracycline and tetracycline. The detection limits of chlortetracycline were 18 渭 g / kg and 30 渭 g / kg, respectively. The average recoveries of chlortetracycline were 76.10% and 95.22% respectively. Conclusion the method has high sensitivity, precision and reproducibility. It is suitable for the determination of oxytetracycline, chlortetracycline and chlortetracycline residues in beef samples.
【作者單位】： 西北民族大學(xué)生命科學(xué)與工程學(xué)院;甘肅省動(dòng)物細(xì)胞工程技術(shù)研究中心;
【基金】：新絲路經(jīng)濟(jì)帶清真食品生產(chǎn)技術(shù)升級(jí)與品牌創(chuàng)新模式研究及應(yīng)用示范(2015BAD29B05) 教育部創(chuàng)新團(tuán)隊(duì)發(fā)展計(jì)劃(IRT13091)
【分類號(hào)】：R155.55
【正文快照】： 四環(huán)素族抗生素是一類由放線菌屬產(chǎn)生的廣譜抗菌劑,土霉素(oxytetracycline,OTC)、四環(huán)素(tetrarcycline,TC)和金霉素(chlortetracycline,CTC)等是其典型代表。四環(huán)素族抗生素對(duì)革蘭陽(yáng)性菌和陰性菌、螺旋體、病毒等有較好的抑制作用,且具有價(jià)格低廉等優(yōu)點(diǎn),常用于畜禽細(xì)菌性疾，

本文編號(hào)：1422360