目的建立急性分離成年犬結(jié)狀神經(jīng)節(jié)神經(jīng)元（VGN）電生理學(xué)研究平臺(tái)。方法通過急性分離和培養(yǎng)雌雄成年比格犬VGN,利用全細(xì)胞膜片鉗技術(shù)分別記錄動(dòng)作電位,電壓依賴性的外向鉀電流（I_K）和超極化激活的陽離子電流（I_h）。結(jié)果動(dòng)作電位波形分析結(jié)果顯示:（1）雌性比格犬VGN不僅可以記錄到傳統(tǒng)分類中的髓鞘化A型和非髓鞘化C型神經(jīng)元,還可以記錄到雌性特異分布的Ah型神經(jīng)元;（2）步階電流除極在誘發(fā)相同持續(xù)放電頻率時(shí),A型所需要的刺激強(qiáng)度最小,Ah型所需要的電流強(qiáng)度與A型近似或輕微增加,而C型需要的刺激強(qiáng)度最大;（3）河豚毒素顯著降低Ah型神經(jīng)元的除極速率和觸及閾值;蝎毒素可顯著增加A-型的神經(jīng)興奮性;（4）所有受試神經(jīng)元均表達(dá)I_K和I_h,且2種電流密度均呈現(xiàn)A型＞Ah型＞C型;（5）盡管雌雄VGN分布的傳入纖維種類有別,已知的A和C型VGN放電差異性并未在本研究中探討。結(jié)論成年比格犬VGN可被成功急性分離,并用于動(dòng)作電位、I_K和I_h的記錄;分類和電生理學(xué)特性足以完成藥理... 

【文章頁數(shù)】：12 頁

【文章目錄】：
1 MATERIALS AND METHODS
    1.1 Animals,reagents and instruments
    1.2 Whole-cell patch-clamp experiments and neuron identification
    1.3 Isolation of vagal ganglion neurons
    1.4 Extracellular solution of AP,repetitive discharge,hyperpolarization-mediated inward currents(Ih) and whole-cell K+currents (Ik)recordings
    1.5 Afferent fiber classification
    1.6 Statistical analysis
2 RESULTS
    2.1 Isolated neurons
    2.2 Action potential discharge characteristics
    2.3 Repetitive discharge profiles
    2.4 Functional expression of voltage-gated Na+channels (TTX-S:Nav1.7,TTX-R:Nav1.8)
    2.5 Functional expression of large conduc-tance Ca2+-activated K+(KCa1.1) channel
    2.6 Functional expression of Ihand IK
3 DISCUSSION


【參考文獻(xiàn)】：
期刊論文
[1]Therapeutic Efficacy of Neurostimulation for Depression:Techniques, Current Modalities, and Future Challenges[J]. Hafsah Akhtar,Faiza Bukhari,Misbah Nazir,Muhammad Nabeel Anwar,Adeeb Shahzad.  Neuroscience Bulletin. 2016(01)
[2]用膜片箝技術(shù)比較大鼠分離細(xì)胞和完整結(jié)狀神經(jīng)節(jié)的動(dòng)作電位(英文)[J]. LIBai-Yan ,JohnHSCHILD.  Acta Pharmacologica Sinica. 2002(06)



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