【摘要】： 目的探討鼻咽癌MRI動態(tài)增強參數(shù)與微血管密度(MVD)和乏氧誘導(dǎo)因子-1α(HIF-α)、血管內(nèi)皮生長因子(VEGF)表達的相關(guān)性。以了解Gd-DTPA動態(tài)增強MRI對診斷腫瘤內(nèi)乏氧和微血管情況的有效性。 方法①14例鼻咽癌初診患者,在鼻咽纖維鏡下標記新生物中一區(qū)域為研究感興趣區(qū)(ROI),行MR動態(tài)增強掃描,獲取該區(qū)域動態(tài)增強曲線；然后對標記處進行鼻咽鏡活檢,測定活檢部位的MVD和HIF-1α、VEGF表達水平；②觀察動態(tài)增強曲線特征,測量ROI的MRI動態(tài)增強半定量參數(shù)對比劑到達腫瘤組織時間(time at arrival of contrast inflow, Tl on set)、到達強化高峰時間(time to peak, TTP)、強化曲線平均斜率及最大斜率(slope and maximum slope,MS)、最大信號強度值(maximum of signal indensity, SImax),并計算ROI的MRI動態(tài)增強定量參數(shù)血漿與EES間的容積轉(zhuǎn)移常量(Ktrans)、血漿與EES間的速率常數(shù)(kep)、EES容積(滲漏空間,ve)；③對各參數(shù)值與MVD和HIF-1α、VEGF表達水平進行相關(guān)性分析。 結(jié)果ROI的半定量參數(shù)slope、MS、SImax與VEGF的表達呈正相關(guān)關(guān)系(r分別為0.889,0.882,0.619,p0.05),與HIF-1α的表達呈正相關(guān)關(guān)系(r分別為0.870,0.825,0.665,p0.05),與MVD也呈正相關(guān)關(guān)系(r分別為0.627,0.755,0.693,p0.05); T1 on set.TTP與VEGF表達呈負相關(guān)關(guān)系(r分別為-0.909,-0.829,p0.05),與HIF-1α表達呈負相關(guān)關(guān)系(r分別為-0.687,-0.690,p0.05),與MVD也呈負相關(guān)關(guān)系(r分別為-0.578,-0.852,p0.05) ROI的定量參數(shù)Ktrans、kep與VEGF的表達呈正相關(guān)關(guān)系(r分別為0.540,0.691,p0.05),與HIF-1α的表達呈正相關(guān)關(guān)系(r分別為0.536,0.677,p0.05)；kep與MVD也呈正相關(guān)關(guān)系(r為0.635, p0.05); Ktrans與MVD無明顯統(tǒng)計學相關(guān)性；ve與VEGF、HIF-1α表達均呈負相關(guān)關(guān)系(r分別為-0.624,-0.595,p0.05),與MVD也呈負相關(guān)關(guān)系(r為-0.667,p0.05) 結(jié)論鼻咽癌活檢部位的MRI動態(tài)增強半定量、定量參數(shù)與其MVD和HIF-1α、VEGF的表達有統(tǒng)計學相關(guān)性；MR動態(tài)增強掃描技術(shù)具有提供鼻咽癌腫瘤內(nèi)乏氧與血管生成信息的能力。
[Abstract]:Objective to investigate the correlation between dynamic enhancement parameters of MRI and the expression of microvessel density (MVD), hypoxia inducible factor-1 偽 (HIF- 偽) and vascular endothelial growth factor (VEGF) in nasopharyngeal carcinoma (NPC). To investigate the effectiveness of Gd-DTPA dynamic contrast-enhanced MRI in the diagnosis of hypoxia and microvessels in tumors. Methods MR dynamic enhancement scan was performed in 114patients with nasopharyngeal carcinoma (NPC) in order to study the region of interest (ROI), and the dynamic enhancement curve of the region was obtained by labeling a region of interest in the new organism under nasopharyngeal fiberscope. Then nasopharyngeal biopsy was performed to detect the expression of MVD, HIF-1 偽 and VEGF in the biopsies. (2) the characteristics of dynamic enhancement curve were observed, and the MRI dynamic enhancement semi-quantitative parameters of ROI were measured. The time (time at arrival of contrast inflow, Tl on set), reached the peak of enhancement (time to peak, TTP),. The mean slope and maximum slope of enhancement curve (slope and maximum slope,MS), the maximum signal intensity value (maximum of signal indensity, SImax), and the MRI dynamic enhancement quantitative parameters of ROI were calculated. The volume transfer constant (Ktrans), between plasma and EES was calculated. Rate constant (kep), EES volume between plasma and EES (leakage space, ve);) (3) the correlation between each parameter value and the expression level of MVD, HIF-1 偽 and VEGF was analyzed. Results the semi-quantitative parameter slope,MS,SImax of ROI was positively correlated with the expression of VEGF (r = 0.889, 0.882, 0.619, p0.05) and with the expression of HIF-1 偽 (r = 0.870, 0.825, 0.665, p0.05, respectively). There was also a positive correlation with MVD (r = 0. 627, 0. 755, 0. 693, p0.05). The expression of T1 on set.TTP was negatively correlated with the expression of VEGF (r =-0.909, 鈮，

本文編號：2496489