【摘要】：目的： 觀察多西他賽對(duì)堿燒傷兔角膜新生血管化的抑制作用及角膜組織中血管內(nèi)皮生長(zhǎng)因子(vascular endothelium growth factor VEGF)的表達(dá)影響，評(píng)價(jià)多西他賽對(duì)兔堿燒傷后角膜新生血管是否具有抑制作用并初步探討其作用機(jī)理，，旨在為該藥的臨床應(yīng)用提供實(shí)驗(yàn)依據(jù),為將來(lái)藥物治療CNV提供一條新途徑。 方法： 新西蘭健康白兔24只，均由南昌大學(xué)實(shí)驗(yàn)動(dòng)物科學(xué)部提供,體質(zhì)量1.5~2.0kg,雌雄兼有;雙眼均為實(shí)驗(yàn)眼。堿燒傷后隨機(jī)將24只兔分為對(duì)照組和3個(gè)實(shí)驗(yàn)組，選擇多西他賽滴眼液藥物質(zhì)量濃度為A組10ug·ml~(-1)；B組20u g·ml~(-1)；c組40u g·ml~(-1)，每組6只12眼，對(duì)照組D組滴NS眼液每日3次作為陰性對(duì)照，共觀察14d。 所有動(dòng)物均于造型后1d開(kāi)始，給予氧氟沙星滴眼液點(diǎn)眼每日4次，連點(diǎn)三日。采用裂隙燈顯微鏡下觀察并測(cè)量新生血管長(zhǎng)度以計(jì)算新生血管化面積、記錄新生血管數(shù)，應(yīng)用免疫組織化學(xué)染色及RT-PCR檢測(cè)血管內(nèi)皮細(xì)胞生長(zhǎng)因子(Vascular endothelial growth factor,VEGF)表達(dá)等方法，研究角膜新生血管的形成及抑制情況。 結(jié)果： （1）觀察結(jié)果：治療組A、B、C組新生血管發(fā)生較遲、生長(zhǎng)緩慢、新生血管化面積小，與對(duì)照組比較，在統(tǒng)計(jì)學(xué)上差異有顯著性（P0.05），治療組B、C組兔角膜新生血管面積低于治療組A和對(duì)照組，在統(tǒng)計(jì)學(xué)上差異有顯著性（P0.05）；（2）免疫組化染色顯示治療組B、C組VEGF的表達(dá)較治療組A和對(duì)照組明顯減少，RT-PCR角膜組織中VEGF mRNA在造模后各時(shí)間點(diǎn)的相對(duì)表達(dá)量，治療組B、C組低于治療組A和對(duì)照組，差異有統(tǒng)計(jì)學(xué)意義（P0.05） 結(jié)論： 多西他賽滴眼液可有效抑制堿燒傷兔角膜新生血管的增殖，并可減少角膜組織VEGF的表達(dá)，抑制效果具有濃度相關(guān)性。
[Abstract]:Objective: to observe the inhibitory effect of docetaxel on corneal neovascularization and the expression of vascular endothelial growth factor (VEGF) (vascular endothelium growth factor VEGF) in corneal tissue after alkali burn in rabbits. To evaluate the inhibitory effect of docetaxel on corneal neovascularization after alkali burn in rabbits and to explore its mechanism in order to provide experimental basis for the clinical application of docetaxel and provide a new way for future drug treatment of CNV. Methods: 24 healthy New Zealand white rabbits were provided by the Department of Experimental Animal Science, Nanchang University, with a body mass of 1.5 to 2.0 kg, both male and female, and both eyes were experimental eyes. After alkali burn, 24 rabbits were randomly divided into control group and three experimental groups. The concentration of docetaxel eye drops was 20 u g 路ml ~ (- 1) in 10ug 路ml ~ (- 1); B group. Group C (40 u g 路ml ~ (- 1), 6 eyes in each group (n = 12) and control group D (n = 6) were treated with NS eye drops 3 times a day as negative control for 14 days. All animals were given ofloxacin eye drops 4 times a day for 3 days. The length of neovascularization was observed and measured under slit lamp microscope to calculate the area of neovascularization, and the number of neovascularization was recorded. The (Vascular endothelial growth factor, of vascular endothelial growth factor (VEGF) was detected by immunohistochemical staining and RT-PCR. The expression of VEGF was used to study the formation and inhibition of corneal neovascularization. Results: (1) the results of observation: the neovascularization of treatment group A, B, C was late, the growth was slow, and the area of neovascularization was small. Compared with the control group, there was significant difference between the two groups (P0.05). The corneal neovascularization area in group C was significantly lower than that in group A and control group (P0.05). (2) Immunohistochemical staining showed that the expression of VEGF in treatment group B, C group was significantly lower than that in treatment group A and control group, and the relative expression of VEGF mRNA in RT-PCR corneal tissue at different time points after modeling, and treatment group B, C group. The difference between group C and control group was statistically significant (P0.05) conclusion: docetaxel eye drops can effectively inhibit the proliferation of corneal neovascularization and reduce the expression of VEGF in corneal tissue of alkali burned rabbits. The inhibitory effect was concentration dependent.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R779.1

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 王淑榮;劉鑫瑤;劉鑫;何宇茜;李瑩;張妍;;角膜新生血管藥物治療進(jìn)展[J];中國(guó)老年學(xué)雜志;2016年05期

本文編號(hào)：2470416