【摘要】： 目的 觀察一種新型的CD4+效應(yīng)T細(xì)胞——輔助性17細(xì)胞(T help 17, Th17)及其相關(guān)免疫學(xué)細(xì)胞因子在變應(yīng)性鼻炎中的表達(dá)情況,以探討其在變應(yīng)性鼻炎發(fā)病機(jī)理中的作用,并為進(jìn)一步研究Th17細(xì)胞的功能調(diào)控機(jī)制奠定基礎(chǔ)。 方法 選擇30例變應(yīng)性鼻炎伴下鼻甲肥大或伴鼻息肉患者作為病例組,25例鼻中隔偏曲并下鼻甲肥大患者作為對(duì)照組,于鼻內(nèi)鏡手術(shù)過(guò)程中嚴(yán)格無(wú)菌操作取鼻粘膜。采用免疫組織化學(xué)法進(jìn)行染色檢測(cè)病例組和對(duì)照組Th17的表達(dá)水平；通過(guò)ELISA法檢測(cè)IL-17、TGF-β在病例組和對(duì)照組的表達(dá)情況,最后進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果 1.Th17陽(yáng)性細(xì)胞的平均水平變應(yīng)性鼻炎患者組和對(duì)照組存在明顯差異(P0.001),Th17在變應(yīng)性鼻炎患者鼻粘膜高表達(dá),對(duì)照組鼻粘膜低表達(dá)。 2.IL-17及TGF-p在變應(yīng)性鼻炎患者組和對(duì)照組存在明顯差異(P0.001),變應(yīng)性鼻炎患者組比對(duì)照組高。 3.變應(yīng)性鼻炎患者組Th17與TGF-p存在正相關(guān)(P0.001)。 結(jié)論 1.變應(yīng)性鼻炎患者中Th17細(xì)胞的高表達(dá)可能是一個(gè)重要原因。 2.TGF-β可能協(xié)同Th17細(xì)胞參與變應(yīng)性鼻炎的發(fā)生發(fā)展。 3.在變應(yīng)性鼻炎中,Th17細(xì)胞TGF-β之間密切相關(guān),或許可以為變應(yīng)性鼻炎的治療提供一條新思路。
[Abstract]:Objective to observe the expression of helper 17 cell (T help 17 (Th17) and its related immunological cytokines in allergic rhinitis (AR), a new type of CD4 effector T cell. In order to explore its role in the pathogenesis of allergic rhinitis, and lay a foundation for further study of the functional regulation mechanism of Th17 cells. Methods 30 patients with allergic rhinitis with inferior turbinate hypertrophy or nasal polyps were selected as the case group and 25 patients with nasal septum deviation and inferior turbinate hypertrophy as the control group. The nasal mucosa was obtained strictly during endoscopic surgery. Immunohistochemical staining was used to detect the expression of Th17 in the case group and control group, and ELISA method was used to detect the expression of IL-17,TGF- 尾 in the case group and the control group. Results the average level of 1.Th17 positive cells was significantly different between the allergic rhinitis group and the control group (P0.001). The expression of Th17 was high in the nasal mucosa of the allergic rhinitis patients, and low in the nasal mucosa of the control group. There was a significant difference in 2.IL-17 and TGF-p between the allergic rhinitis group and the control group (P0.001), and it was higher in the allergic rhinitis group than that in the control group. 3. There was a positive correlation between Th17 and TGF-p in patients with allergic rhinitis (P0.001). Conclusion 1. The high expression of Th17 cells in patients with allergic rhinitis may be an important cause. 2. TGF-尾 may cooperate with Th17 cells in the development of allergic rhinitis. 3. In allergic rhinitis, TGF- 尾 of Th17 cells is closely related, which may provide a new idea for the treatment of allergic rhinitis.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類(lèi)號(hào)】：R765.21

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