糖康對糖尿病視網(wǎng)膜病變的影響及其作用機(jī)制研究
發(fā)布時(shí)間：2019-03-22 10:35
【摘要】： 目的:從視網(wǎng)膜微血管形態(tài)學(xué)和細(xì)胞分子兩個(gè)水平,觀察糖康對糖尿病大鼠視網(wǎng)膜病變的影響,并探討其具體作用機(jī)制,為糖康防治糖尿病視網(wǎng)膜病變提供理論依據(jù)。方法:采用腹腔注射鏈脲佐菌素復(fù)制糖尿病模型,并隨機(jī)分為正常對照組、模型組、糖康高、中和低劑量組和達(dá)納康組,每日灌胃,定期檢測大鼠體重和非空腹血糖。第12周末,消化鋪片法觀察視網(wǎng)膜微血管形態(tài)學(xué)改變;化學(xué)比色法檢測各組大鼠血清及視網(wǎng)膜組織中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。Western blot檢測NF-κB亞基P65在視網(wǎng)膜組織細(xì)胞核內(nèi)的含量。半定量RT-PCR檢測其上、下游靶基因IL-1β、Bax和VEGF的表達(dá)水平。結(jié)果:經(jīng)STZ誘發(fā)的糖尿病大鼠模型,造模一周左右即出現(xiàn)DM典型的“三多”癥狀。糖康高、中劑量組和與模型組相比,上述癥狀輕于模型組,而糖康低劑量組和達(dá)納康組與同期模型組相比無明顯變化;模型組與各治療組大鼠較同期正常組大鼠體重明顯降低(P0.01);正常組大鼠血糖保持穩(wěn)定,模型組與各治療組大鼠較同期正常組大鼠組血糖明顯升高(P0.01);與正常組比較,模型組大鼠視網(wǎng)膜呈現(xiàn)較多的無細(xì)胞毛細(xì)血管條索數(shù)(約為正常組的12.16倍)。與模型組比較,糖康高、中、低劑量組和達(dá)納康組無細(xì)胞毛細(xì)血管條索數(shù)明顯減少(P0.01);與模型組比較,各治療組大鼠血清及視網(wǎng)膜組織的SOD活性均不同程度升高,MDA含量均不同程度降低,以糖康高劑量組效果最為顯著(P0.01);與正常對照組比較,模型組NF-κBP65蛋白表達(dá)水平明顯上調(diào)(P0.01),各治療組NF-κBP65蛋白在視網(wǎng)膜組織的表達(dá)不同程度降低,以糖康高劑量組最為顯著;與正常組比較,模型組大鼠視網(wǎng)膜IL-1β、VEGF和Bax的mRNA表達(dá)明顯上調(diào)(P0.01)。與模型組比較,各治療組大鼠視網(wǎng)膜IL-1β、VEGF和Bax的mRNA表達(dá)均不同程度降低,以糖康高劑量組最為顯著。結(jié)論:用STZ誘發(fā)糖尿病大鼠模型是一種快速、簡便、有效的動(dòng)物模型制備方法,STZ最佳用藥濃度為50 mg/kg;糖康能夠改善DM大鼠的諸多糖尿病癥狀,但對血糖和體重改善不明顯;糖康能夠明顯抑制糖尿病誘發(fā)的視網(wǎng)膜微血管細(xì)胞的凋亡和無細(xì)胞毛細(xì)血管條索數(shù)的增加,對糖尿病大鼠視網(wǎng)膜微血管具有一定的保護(hù)作用;糖康能夠顯著增加糖尿病大鼠血清及視網(wǎng)膜SOD活性,降低MDA含量,說明該藥物具有清除糖尿病大鼠體內(nèi)自由基,增強(qiáng)機(jī)體抗氧化防御能力,調(diào)節(jié)和改善自由基代謝的作用;糖康對糖尿病大鼠視網(wǎng)膜的保護(hù)作用可能與減少NF-κBP65在細(xì)胞核內(nèi)的蛋白表達(dá)有關(guān);糖康對糖尿病大鼠視網(wǎng)膜的保護(hù)作用可能與下調(diào)IL-1β、VEGF和Bax的基因表達(dá)有關(guān)。
[Abstract]:Objective: To observe the effect of Tangkang on the diabetic retinopathy in diabetic rats from two levels of retinal microvessel morphology and cell molecule, and to provide a theoretical basis for the prevention and treatment of diabetic retinopathy. Methods: The model of diabetes mellitus was duplicated by intraperitoneal injection of streptozoin. The rats were randomly divided into the normal control group, the model group, the sugar-kang group, the middle and low-dose group and the Dana-kang group, and the body weight and the non-fasting blood sugar were detected on a regular basis. The changes of the microvessel morphology of the retina were observed at the end of the 12th week, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the serum and retinal tissues of each group were detected by a chemical colorimetric method. Western blot was used to detect the content of NF-VIB subunit P65 in the nucleus of the retinal tissue. The expression levels of IL-1, Bax and VEGF were detected by semi-quantitative RT-PCR. Results: The model of diabetic rats induced by STZ was the typical "Sando" of DM in about one week. The above-mentioned symptoms were mild in the model group compared with the model group, while the low-dose group and the Dana-kang group did not change significantly compared with the model group, and the weight of rats in the model group and the treatment group was significantly lower than that in the normal group (P0.01). In the normal group, the blood glucose remained stable, and the blood sugar in the model group and the control group was significantly higher than that in the normal group (P 0.01). Compared with the normal group, the rat retina of the model group exhibited more cell-free capillary strips (about 12.16 times the normal group). Compared with the model group, there was a significant decrease in the number of cell-free capillary cells in the high, middle and low-dose group and in the Dana-kang group (P0.01). Compared with the model group, the activity of SOD in the serum and retinal tissues of the rats in the treatment group increased significantly, and the content of MDA decreased. Compared with the normal control group, the expression of NF-BBP65 protein in the model group was up-regulated (P0.01), and the expression of NF-BBP65 protein in each treatment group was lower in the retinal tissue, and the group was the most significant in the high-dose group. Compared with the normal group, the expression of IL-1, VEGF and Bax in rat retina was up-regulated (P0.01). Compared with the model group, the expression of IL-1, VEGF and Bax in the retina of each treatment group was decreased to a different extent, and the group was the most significant in the high-dose group. Conclusion: The model of STZ-induced diabetic rats is a fast, simple and effective method for preparing animal model. The optimal concentration of STZ is 50 mg/ kg. The sugar-kang can obviously inhibit the apoptosis of the retinal microvessel cells induced by diabetes and the increase of the number of the cell-free capillary strips, have a certain protective effect on the diabetic rat retina microvessel, and can remarkably increase the SOD activity of the serum and the retina of the diabetic rats, The content of MDA was decreased, which indicated that the drug had the effects of scavenging free radicals in diabetic rats, enhancing the anti-oxidative defense ability of the body, regulating and improving the free-radical metabolism, and the protective effect of the sugar-kang on the retina of the diabetic rats may be related to the reduction of the expression of the NF-BBP65 in the nucleus of the nucleus; The protective effect of Tangkang on the retina of diabetic rats may be related to the down-regulation of the expression of IL-1, VEGF and Bax.
【學(xué)位授予單位】：新疆農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R587.1;R774.1
本文編號：2445517
[Abstract]:Objective: To observe the effect of Tangkang on the diabetic retinopathy in diabetic rats from two levels of retinal microvessel morphology and cell molecule, and to provide a theoretical basis for the prevention and treatment of diabetic retinopathy. Methods: The model of diabetes mellitus was duplicated by intraperitoneal injection of streptozoin. The rats were randomly divided into the normal control group, the model group, the sugar-kang group, the middle and low-dose group and the Dana-kang group, and the body weight and the non-fasting blood sugar were detected on a regular basis. The changes of the microvessel morphology of the retina were observed at the end of the 12th week, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the serum and retinal tissues of each group were detected by a chemical colorimetric method. Western blot was used to detect the content of NF-VIB subunit P65 in the nucleus of the retinal tissue. The expression levels of IL-1, Bax and VEGF were detected by semi-quantitative RT-PCR. Results: The model of diabetic rats induced by STZ was the typical "Sando" of DM in about one week. The above-mentioned symptoms were mild in the model group compared with the model group, while the low-dose group and the Dana-kang group did not change significantly compared with the model group, and the weight of rats in the model group and the treatment group was significantly lower than that in the normal group (P0.01). In the normal group, the blood glucose remained stable, and the blood sugar in the model group and the control group was significantly higher than that in the normal group (P 0.01). Compared with the normal group, the rat retina of the model group exhibited more cell-free capillary strips (about 12.16 times the normal group). Compared with the model group, there was a significant decrease in the number of cell-free capillary cells in the high, middle and low-dose group and in the Dana-kang group (P0.01). Compared with the model group, the activity of SOD in the serum and retinal tissues of the rats in the treatment group increased significantly, and the content of MDA decreased. Compared with the normal control group, the expression of NF-BBP65 protein in the model group was up-regulated (P0.01), and the expression of NF-BBP65 protein in each treatment group was lower in the retinal tissue, and the group was the most significant in the high-dose group. Compared with the normal group, the expression of IL-1, VEGF and Bax in rat retina was up-regulated (P0.01). Compared with the model group, the expression of IL-1, VEGF and Bax in the retina of each treatment group was decreased to a different extent, and the group was the most significant in the high-dose group. Conclusion: The model of STZ-induced diabetic rats is a fast, simple and effective method for preparing animal model. The optimal concentration of STZ is 50 mg/ kg. The sugar-kang can obviously inhibit the apoptosis of the retinal microvessel cells induced by diabetes and the increase of the number of the cell-free capillary strips, have a certain protective effect on the diabetic rat retina microvessel, and can remarkably increase the SOD activity of the serum and the retina of the diabetic rats, The content of MDA was decreased, which indicated that the drug had the effects of scavenging free radicals in diabetic rats, enhancing the anti-oxidative defense ability of the body, regulating and improving the free-radical metabolism, and the protective effect of the sugar-kang on the retina of the diabetic rats may be related to the reduction of the expression of the NF-BBP65 in the nucleus of the nucleus; The protective effect of Tangkang on the retina of diabetic rats may be related to the down-regulation of the expression of IL-1, VEGF and Bax.
【學(xué)位授予單位】：新疆農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R587.1;R774.1
【引證文獻(xiàn)】
相關(guān)博士學(xué)位論文 前1條
1 郭藝娟;益氣養(yǎng)血通絡(luò)法干預(yù)糖尿病視網(wǎng)膜病變的臨床與實(shí)驗(yàn)研究[D];中國中醫(yī)科學(xué)院;2012年
，本文編號：2445517
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