【摘要】：目的：研究中耳膽脂瘤上皮組織中IL-6和P-STAT3的表達(dá)及其相關(guān)性,探討IL-6和P-STAT3在中耳膽脂瘤發(fā)生和發(fā)展中的作用。 方法：采用免疫組織化學(xué)SP法(SP immunohistochemical method)檢測(cè)25例成人中耳膽脂瘤上皮組織中IL-6和P-STAT3蛋白的表達(dá)情況,另取其中15例患者的正常外耳道皮膚組織作為對(duì)照。應(yīng)用SPSS15.0軟件進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果：25例中耳膽脂瘤上皮組織中,IL-6蛋白表達(dá)陽(yáng)性18例(72.00%), P-STAT3蛋白表達(dá)陽(yáng)性16例(64.00%)。15例對(duì)照組標(biāo)本中IL-6和P-STAT3蛋白陽(yáng)性表達(dá)分別為3例(20.00%)和4例(26.67%),兩組差異具有統(tǒng)計(jì)學(xué)意義(P0.05)；中耳膽脂瘤組IL-6和P-STAT3的表達(dá)具有正相關(guān)性(r=0.46,P0.05)。 結(jié)論：IL-6、P-STAT3在中耳膽脂瘤上皮組織中呈異常高表達(dá),提示IL-6蛋白和P-STAT3蛋白過(guò)度表達(dá)可能與膽脂瘤上皮的高度增殖狀態(tài)密切相關(guān),在中耳膽脂瘤的發(fā)生與發(fā)展中發(fā)揮著一定作用。IL-6蛋白和P-STAT3蛋白表達(dá)呈正相關(guān),提示IL-6-STAT3信號(hào)傳導(dǎo)通路在中耳膽脂瘤上皮的過(guò)度增殖機(jī)制中起到重要作用。
[Abstract]:Objective: to investigate the expression of IL-6 and P-STAT3 in middle ear cholesteatoma and their correlation, and to explore the role of IL-6 and P-STAT3 in the pathogenesis and development of middle ear cholesteatoma. Methods: the expression of IL-6 and P-STAT3 protein in 25 cases of adult middle ear cholesteatoma was detected by immunohistochemical SP (SP immunohistochemical method) method, and 15 cases of normal external auditory canal skin tissue were used as control. SPSS15.0 software was used for statistical analysis. Results: in 25 cases of middle ear cholesteatoma, 18 cases (72.00%) were positive for IL-6 protein expression. There were 16 cases (64.00%) positive expression of P-STAT3 protein, 3 cases (20.00%) of IL-6 and 4 cases (26.67%) of P-STAT3 protein in 15 cases of control group, the difference was statistically significant (P0.05). There was a positive correlation between the expression of IL-6 and P-STAT3 in middle ear cholesteatoma group (P 0.05). Conclusion: the overexpression of IL-6,P-STAT3 in the epithelium of middle ear cholesteatoma suggests that the overexpression of IL-6 protein and P-STAT3 protein may be closely related to the high proliferation of cholesteatoma epithelium. The expression of IL-6 protein and P-STAT3 protein were positively correlated, suggesting that IL-6-STAT3 signal transduction pathway plays an important role in the mechanism of excessive proliferation of middle ear cholesteatoma epithelium.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R764

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本文編號(hào)：2394708