【摘要】：目的通過(guò)觀察兔眼角膜真菌感染后角膜新生血管形成規(guī)律,探討角膜新生血管形成與角膜炎預(yù)后的關(guān)系。 方法選取7只健康新西蘭大白兔,制作標(biāo)準(zhǔn)真菌性角膜炎動(dòng)物模型。每天在裂隙燈顯微鏡下觀察兔眼大體改變,分別記錄真菌潰瘍形成后不同時(shí)間段兔眼的病程進(jìn)展;分別于真菌潰瘍形成后第3、7、15和30天,行病理學(xué)和超微結(jié)構(gòu)檢查。 結(jié)果兔眼真菌潰瘍形成后第1天和第3天,可見有較重的炎癥表現(xiàn);第7天時(shí),均有大量角膜周邊新生血管長(zhǎng)入;第15天時(shí),球結(jié)膜充血減輕,角膜新生血管有所緩解,角膜潰瘍未見減輕;第30天時(shí),有較輕的眼部炎癥,角膜周邊殘存少量新生血管。病理學(xué)檢查顯示:真菌潰瘍形成后第3天,重度炎癥病理表現(xiàn),毛細(xì)血管擴(kuò)張,無(wú)新生血管;第7天,有角膜新生血管形成;第15天炎癥局限,有成熟的新生血管;第30天,新生血管減少。超微結(jié)構(gòu)觀察:真菌潰瘍形成后第3天和第7天,典型炎癥的超微結(jié)構(gòu)改變;第15天,基質(zhì)層仍可見到真菌菌絲,但較前明顯減少,角膜上皮細(xì)胞核和細(xì)胞器異常;第30天,輕度炎癥表現(xiàn),未見真菌菌絲。 結(jié)論1兔眼真菌性角膜炎在不應(yīng)用任何抗真菌藥物的情況下具有自愈轉(zhuǎn)歸的特點(diǎn)。2兔眼角膜新生血管與真菌性角膜炎的恢復(fù)有相關(guān)性。
[Abstract]:Objective to investigate the relationship between corneal neovascularization and keratitis prognosis by observing corneal neovascularization after fungal infection. Methods 7 healthy New Zealand rabbits were selected to make standard fungal keratitis animal model. The gross changes of rabbit eyes were observed under slit-lamp microscope every day, and the progress of the disease course of rabbit eyes was recorded at different times after the formation of fungal ulcers, and the pathological and ultrastructural examinations were performed on the 15th and 30th days after the formation of fungal ulcers. Results on the 1st and 3rd day after the formation of fungal ulcer in rabbit eyes, severe inflammation was observed, and on the 7th day, a large number of neovascularization appeared around the cornea. On the 15th day, the bulbar conjunctiva hyperemia was alleviated, corneal neovascularization was alleviated, corneal ulcer was not alleviated, on the 30th day, there was mild ocular inflammation and a small amount of neovascularization around the cornea. Pathological examination showed that on the third day after the formation of fungal ulcer, severe inflammatory pathological manifestations were found, capillary dilated without neovascularization, corneal neovascularization on day 7, inflammation localized on day 15, and mature neovascularization on day 15. On day 30, neovascularization was reduced. Ultrastructural observation: on the 3rd and 7th day after the formation of fungal ulcer, the ultrastructural changes of typical inflammation were observed, and on the 15th day, mycelium could still be seen in the stroma layer, but the number of mycelium was significantly decreased, and the keratoepithelium nucleus and organelle were abnormal. On the 30th day, mild inflammation and no fungal hyphae were found. Conclusion (1) mycological keratitis has the characteristics of self-healing without any antifungal drugs. 2 the corneal neovascularization is correlated with the recovery of fungal keratitis.
【學(xué)位授予單位】：寧夏醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R772.21

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