【摘要】：目的探討miR-182誘導(dǎo)耳蝸前體細(xì)胞向毛細(xì)胞分化的作用及機(jī)制。方法從新生大鼠耳蝸中分離培養(yǎng)耳蝸前體細(xì)胞并用血清誘導(dǎo)分化,BrdU、Nestin免疫熒光染色鑒定細(xì)胞自我增殖能力;myosinⅦa和phalloidin免疫熒光染色鑒定其是否具有分化為毛細(xì)胞的潛能。分別利用miR-182 mimics和siRNA在新生大鼠耳蝸前體細(xì)胞中過表達(dá)和低表達(dá)miR-182,然后在細(xì)胞誘導(dǎo)分化7天后,用流式細(xì)胞儀檢測分化細(xì)胞中myosinⅦa陽性細(xì)胞比例,用Western-blot檢測支持細(xì)胞標(biāo)志分子中Sox2的變化。結(jié)果使用miR-182 mimics進(jìn)行過表達(dá)后,耳蝸前體細(xì)胞分化為myosinⅦa陽性表達(dá)的細(xì)胞比例顯著高于對(duì)照組,使用miR-182 siRNA進(jìn)行低表達(dá)后此比例顯著低于對(duì)照組。Western-blot檢測顯示,Sox2在miR-182 mimics組較對(duì)照組降低,miR-182 siRNA組較對(duì)照組增加。結(jié)論過表達(dá)miRN182可以促進(jìn)耳蝸前體細(xì)胞向毛細(xì)胞方向分化,ox2可能是此過程中的靶基因。
[Abstract]:Objective to investigate the role and mechanism of miR-182 in inducing cochlear precursor cells to differentiate into hair cells. Methods cochlear progenitor cells were isolated from neonatal rat cochlea and differentiated by serum. BrdU,Nestin immunofluorescence staining was used to identify the self-proliferation ability of the cells, and myosin 鈪，

本文編號(hào)：2376704