【摘要】：目的探討miR-182誘導耳蝸前體細胞向毛細胞分化的作用及機制。方法從新生大鼠耳蝸中分離培養(yǎng)耳蝸前體細胞并用血清誘導分化,BrdU、Nestin免疫熒光染色鑒定細胞自我增殖能力;myosinⅦa和phalloidin免疫熒光染色鑒定其是否具有分化為毛細胞的潛能。分別利用miR-182 mimics和siRNA在新生大鼠耳蝸前體細胞中過表達和低表達miR-182,然后在細胞誘導分化7天后,用流式細胞儀檢測分化細胞中myosinⅦa陽性細胞比例,用Western-blot檢測支持細胞標志分子中Sox2的變化。結(jié)果使用miR-182 mimics進行過表達后,耳蝸前體細胞分化為myosinⅦa陽性表達的細胞比例顯著高于對照組,使用miR-182 siRNA進行低表達后此比例顯著低于對照組。Western-blot檢測顯示,Sox2在miR-182 mimics組較對照組降低,miR-182 siRNA組較對照組增加。結(jié)論過表達miRN182可以促進耳蝸前體細胞向毛細胞方向分化,ox2可能是此過程中的靶基因。
[Abstract]:Objective to investigate the role and mechanism of miR-182 in inducing cochlear precursor cells to differentiate into hair cells. Methods cochlear progenitor cells were isolated from neonatal rat cochlea and differentiated by serum. BrdU,Nestin immunofluorescence staining was used to identify the self-proliferation ability of the cells, and myosin 鈪，

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