【摘要】：目的：研究鼻咽癌（nasopharyngeal carcinoma，NPC）臨床標(biāo)本中NFBD1（nuclear factor with BRCT domains protein1）的表達(dá)水平及其臨床意義，探討NFBD1表達(dá)下調(diào)對鼻咽癌細(xì)胞生長、周期、裸鼠移植瘤生長速度的影響。 方法：IHC法測量鼻咽癌組織（40例）及慢性鼻咽炎組織(20例)中蛋白水平上NFBD1的表達(dá)，實時熒光定量PCR檢測34例鼻咽癌組織和23例慢性鼻咽炎組織中NFBD1mRNA的表達(dá)水平，，并分析患者性別、年齡、頸淋巴結(jié)轉(zhuǎn)移和臨床分期與之的相關(guān)性；培養(yǎng)鼻咽癌細(xì)胞株CNE-1，逆轉(zhuǎn)錄病毒介導(dǎo)的siRNA感染CNE-1細(xì)胞后，通過實時熒光定量PCR及免疫印跡（Western blot）檢測NFBD1的表達(dá)水平變化，采用CCK-8法檢測NFBD1siRNA對CNE-1細(xì)胞生長速度的改變，流式細(xì)胞術(shù)檢測NFBD1siRNA對細(xì)胞周期的影響，并觀察其對裸鼠皮下移植瘤生長速度的影響。 結(jié)果：鼻咽癌組織中NFBD1在蛋白水平(x2=9.600,P=0.002）和mRNA水平（t=3.206，P=0.002）表達(dá)均顯著高于慢性鼻咽炎組織,NFBD1在mRNA水平的表達(dá)高低與患者的性別、年齡及是否伴有臨床頸淋巴結(jié)轉(zhuǎn)移、臨床分期均沒有相關(guān)性。NFBD1siRNA可以下調(diào)NFBD1在mRNA（t=22.517，P=0.002）及蛋白水平的表達(dá)，并可以抑制CNE-1細(xì)胞的生長增殖速度、阻滯細(xì)胞于細(xì)胞周期的G2M期，抑制CNE-1裸鼠皮下移植瘤的生長速度（t=5.563，P=0.003）。 結(jié)論：NFBD1在鼻咽癌組織中的表達(dá)高于慢性鼻咽炎組織，抑制NFBD1表達(dá)可以抑制細(xì)胞生長速度、改變細(xì)胞周期、降低裸鼠移植瘤生長速度，其可能參與了鼻咽癌的發(fā)生，促進(jìn)其生長，但其與鼻咽癌的侵襲轉(zhuǎn)移沒有明顯聯(lián)系。
[Abstract]:Objective: to study the expression of NFBD1 (nuclear factor with BRCT domains protein1 in clinical specimens of nasopharyngeal carcinoma (nasopharyngeal carcinoma,NPC) and its clinical significance, and to explore the effect of down-regulation of NFBD1 expression on the growth, cycle and growth rate of nude mice xenografts. Methods: the expression of NFBD1 was measured by IHC in 40 cases of nasopharyngeal carcinoma (NPC) and 20 cases of chronic nasopharyngitis, and the expression of NFBD1mRNA in 34 cases of nasopharyngeal carcinoma and 23 cases of chronic nasopharyngitis was detected by real-time fluorescence quantitative PCR. The correlation between sex, age, cervical lymph node metastasis and clinical stage was analyzed. After CNE-1 cells were infected with siRNA mediated by CNE-1, retrovirus, the expression of NFBD1 was detected by real-time fluorescence quantitative PCR and Western blot (Western blot). CCK-8 assay was used to detect the change of growth rate of CNE-1 cells induced by NFBD1siRNA, and flow cytometry was used to detect the effect of NFBD1siRNA on cell cycle, and to observe the effect of NFBD1siRNA on the growth rate of subcutaneous transplanted tumor in nude mice. Results: the expression of NFBD1 and mRNA in nasopharyngeal carcinoma was significantly higher than that in chronic nasopharyngitis. The expression of NFBD1 in mRNA was significantly higher than that in chronic nasopharyngitis. There was no correlation between age and clinical cervical lymph node metastasis. NFBD1siRNA could down-regulate the expression of NFBD1 in mRNA and protein levels, and inhibit the growth and proliferation of CNE-1 cells. The growth rate of subcutaneous transplanted tumor in CNE-1 nude mice was inhibited by blocking the cell cycle in G 2m phase (t = 5.563P0. 003). Conclusion: the expression of NFBD1 in nasopharyngeal carcinoma is higher than that in chronic nasopharyngitis. Inhibition of NFBD1 expression can inhibit cell growth, change cell cycle and decrease growth rate of transplanted tumor in nude mice, which may be involved in the occurrence of nasopharyngeal carcinoma. Promote its growth, but it has no obvious relationship with invasion and metastasis of nasopharyngeal carcinoma.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R739.63

【共引文獻(xiàn)】

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1 劉峰;鼻咽癌殘存病灶分次立體定向放射治療臨床研究[D];北京協(xié)和醫(yī)學(xué)院;2013年

2 陳宇;CTMP在七氟醚預(yù)處理神經(jīng)保護(hù)中的作用機(jī)制研究[D];第四軍醫(yī)大學(xué);2013年

3 陳亮;miR-421下調(diào)FOXO4促進(jìn)鼻咽癌細(xì)胞增殖及凋亡抵抗[D];南方醫(yī)科大學(xué);2013年

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1 周杰;馬來酰亞胺聚谷氨酸天冬氨酸靶向肽聚合物藥物載體系統(tǒng)的建立與應(yīng)用[D];華東師范大學(xué);2013年

2 劉凱;多聚L-谷氨酸-γ-芐酯納米粒子提高耐藥細(xì)胞藥物累積的研究[D];華東師范大學(xué);2013年

3 阮開安;鼻咽癌放療后局部復(fù)發(fā)的影像學(xué)分析[D];廣西醫(yī)科大學(xué);2013年

4 林慶良;轉(zhuǎn)移性鼻咽癌化療方案的臨床研究[D];福建醫(yī)科大學(xué);2013年

5 黃新義;siRNA沉默Survivin對鼻咽癌細(xì)胞CNE2的影響[D];南華大學(xué);2013年

本文編號：2370206