【摘要】：目的觀察α-硫辛酸對(duì)光損傷后小鼠視網(wǎng)膜的保護(hù)作用。方法將42只成年健康BALB/C小鼠隨機(jī)分為正常組、光損傷組和藥物干預(yù)組（干預(yù)組又分為造模前后均給藥組及造模后給藥組）。除正常組外其他組小鼠均暴露于裝有白色光源的光損傷箱內(nèi)，光暴露時(shí)間為12h，之后暗室飼養(yǎng)12h，共3個(gè)循環(huán)，光照強(qiáng)度21400±1600 lx，總暴露時(shí)間為36h。箱內(nèi)溫度控制在25-29℃。藥物干預(yù)組腹腔注射α-硫辛酸注射液100mg/Kg，一次/日，直至處死。分別于7d及14 d處死動(dòng)物摘取眼球，制備眼球壁石蠟切片，在光學(xué)顯微鏡下觀察其形態(tài)學(xué)變化，，測(cè)量視網(wǎng)膜外核層厚度，用TUNEL法計(jì)數(shù)外核層細(xì)胞凋亡數(shù)及凋亡指數(shù)，用x±s表示，各組之間比較用兩因素析因設(shè)計(jì)的方差分析。結(jié)果與光損傷組相比，藥物干預(yù)組視網(wǎng)膜結(jié)構(gòu)層次分明，細(xì)胞排列整齊。其中視網(wǎng)膜外核層厚度，正常組41.62±0.77um,光損傷組7天30.51±0.73um，14天20.04±0.52um，藥物干預(yù)組（C1組）7天40.52±0.80um，14天41.43±0.72um，造模后給藥組（C2組）7天31.70±0.0.70um，14天38.92±1.43um，顯著高于光損傷組，（p0.001）。Tunel試劑盒檢測(cè)細(xì)胞凋亡結(jié)果顯示：正常組AI1,光損傷組AI=15.44±1.72，藥物干預(yù)Ⅰ組AI=7.23±0.65，藥物干預(yù)Ⅱ組AI=7.48±0.88。給藥組小鼠視網(wǎng)膜外核層的凋亡細(xì)胞數(shù)明顯少于光損傷組小鼠。外核層（ONL）厚度及凋亡指數(shù)（AI）的組間差異具有統(tǒng)計(jì)學(xué)意義。結(jié)論在本實(shí)驗(yàn)條件下，α-硫辛酸對(duì)小鼠視網(wǎng)膜光損傷具有保護(hù)作用。
[Abstract]:Objective to observe the protective effect of 偽-lipoic acid on the retina of mice after light injury. Methods 42 healthy adult BALB/C mice were randomly divided into three groups: normal group, light injury group and drug intervention group. All the mice in the other groups except the normal group were exposed to light damage box with white light source for 12 hours, then the dark room was fed for 12 hours, and the light intensity was 21400 鹵1600 lx,. The total exposure time was 36 hours. The temperature in the box is controlled at 25-29 鈩

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