【摘要】：目的:探討超聲造影劑在家兔眼部睫狀循環(huán)的顯影并研究頸動脈狹窄與前部缺血性視神經(jīng)病變(anterior ischemic optic neuropathy,AION)的關(guān)系。方法:將36只健康雄性家兔隨機(jī)分為手術(shù)組(20只40眼)、假手術(shù)組(8只16眼)、空白對照組(8只16眼)。手術(shù)組采取家兔頸動脈部分縫合術(shù)建立頸動脈狹窄的動物模型,假手術(shù)組行頸動脈臨時夾閉,空白組不做任何處理。分別于術(shù)后3、7、14、21 d和35 d行頸動脈彩色多普勒超聲檢查、超聲造影劑睫狀微循環(huán)顯影與視神經(jīng)組織病理學(xué)分析。結(jié)果:手術(shù)組家兔造模成功,術(shù)后均出現(xiàn)不同程度頸動脈狹窄。3、7、14、21 d和35 d頸動脈內(nèi)徑值分別為(0.74±0.15)、(0.74±0.15)、(0.81±0.20)、(0.88±0.20)mm和(1.05±0.29)mm,較假手術(shù)組與空白對照組低,差異無統(tǒng)計學(xué)意義(P0.05)。超聲造影劑在睫狀動脈顯影良好,手術(shù)組單個超聲微泡移動速度在各時間點分別為(7.10±2.12)、(8.20±2.39)、(8.11±2.24)、(7.46±2.19)mm/s和(10.11±3.14)mm/s,較假手術(shù)組及空白對照組低,差異有統(tǒng)計學(xué)意義(P0.05)。光學(xué)顯微鏡下可見手術(shù)組視神經(jīng)纖維排列紊亂,軸突腫脹呈空泡狀,細(xì)胞質(zhì)著色淺,炎性細(xì)胞浸潤。結(jié)論:通過超聲造影劑微循環(huán)顯影可觀察到頸動脈狹窄的家兔睫狀動脈血流速率下降,并出現(xiàn)視神經(jīng)的組織病理學(xué)改變,提示頸動脈狹窄可以引起缺血性視神經(jīng)病變。
[Abstract]:Objective: to study the relationship between carotid stenosis and anterior ischemic optic neuropathy (anterior ischemic optic neuropathy,AION). Methods: 36 healthy male rabbits were randomly divided into operation group (20 eyes, 40 eyes), sham operation group (8 eyes, 16 eyes) and blank control group (8 eyes, 16 eyes). The animal model of carotid artery stenosis was established in the operation group by partial suture of the carotid artery in rabbits, the temporary occlusion of the carotid artery was performed in the sham operation group, and no treatment was done in the blank group. Carotid color Doppler ultrasonography, ciliary microcirculation imaging and histopathological analysis of optic nerve were performed on 21 and 35 days after operation. Results: the model was successfully established in the operation group, and the carotid artery stenosis of different degree appeared after operation. The internal diameter of carotid artery was (0.74 鹵0.15), (鹵0.74 鹵0.81 鹵0.20) at 21 and 35 days after operation, respectively. (0. 88 鹵0. 20) mm and (1. 05 鹵0. 29) mm, were lower than those of sham operation group and blank control group (P0.05). Ultrasound contrast medium showed well in ciliary artery. The velocity of single ultrasound microbubble in operation group was (7.10 鹵2.12), () 8.20 鹵2.39), (8.11 鹵2.24). (7.46 鹵2.19) mm/s and (10.11 鹵3.14) mm/s, were significantly lower than those in sham operation group and blank control group (P0.05). Under the optical microscope, the optic nerve fibers in the operation group were disordered, axon swelling was vacuolar, cytoplasm was light and inflammatory cells were infiltrated. Conclusion: the decrease of blood flow rate of ciliary artery and histopathological changes of optic nerve in rabbits with carotid artery stenosis can be observed by ultrasound contrast medium microcirculation, suggesting that carotid artery stenosis may cause ischemic optic neuropathy.
【作者單位】： 重慶醫(yī)科大學(xué)附屬第二醫(yī)院眼科;
【基金】：重慶市醫(yī)學(xué)科研計劃資助項目(編號:2010-1-26)
【分類號】：R774.6

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