LBA在剝奪性弱視大鼠視覺發(fā)育可塑性關(guān)鍵期的作用
發(fā)布時(shí)間：2018-11-11 16:10
【摘要】：目的建立單眼視覺剝奪性弱視大鼠動物模型,研究枸杞子提取物(LBA)對視覺剝奪性弱視大鼠視網(wǎng)膜神經(jīng)節(jié)細(xì)胞(RGCs)是否具有保護(hù)作用。 方法新生Wistar大鼠30只,隨機(jī)分成生理鹽水組(10只)、左旋多巴組(10只)及LBA組(10只)。出生1周后,應(yīng)用褥式縫合法縫合所有幼鼠左側(cè)眼瞼,建立單眼視覺剝奪性弱視動物模型,三組每日分別經(jīng)口給予等量的NS (0.9% NaCl)、L-dopa(20mg/kg)及LBA(10mg/kg),喂養(yǎng)滿6周。取材前5天將30g/L熒光金注射到所有大鼠雙側(cè)的上丘和外側(cè)膝狀體,通過熒光金逆行標(biāo)記觀察視網(wǎng)膜鋪片中RGCs形態(tài)和數(shù)量的變化。 結(jié)果①視網(wǎng)膜鋪片中標(biāo)記的RGCs為黃白色,胞體形態(tài)多樣,可見熒光充盈的細(xì)胞突起。②NS組、L-dopa組及LBA組中左眼的RGCs密度明顯低于右眼,差異均有顯著性(P0.01)。③與NS組相比,L-dopa組及LBA組左眼RGCs密度均明顯升高(P0.01),而L-dopa組及LBA組之間差異無顯著性(P0.05)。④L-dopa組及LBA組RGCs的存活率分別為85.17%±4.42%和84.31%±5.97%,均明顯高于NS組71.35%±6.34%(P0.01),而L-dopa組及LBA組之間差異無顯著性(P0.05)。 結(jié)論①在剝奪性弱視大鼠視覺發(fā)育可塑性的關(guān)鍵期,LBA可有效地保護(hù)RGCs,提高RGCs存活率。②LBA對RGCs的視覺保護(hù)作用與治療弱視的常規(guī)用藥左旋多巴效果接近。 目的建立單眼剝奪性弱視大鼠動物模型,研究視覺發(fā)育敏感期不同階段枸杞子提取物(LBA)對視皮層17區(qū)Bcl-2和NGF表達(dá)的影響。 方法新生Wistar大鼠60只,隨機(jī)分成正常組(12只)、生理鹽水組(24只)、LBA組(24只)。出生7天后,應(yīng)用褥式縫合法縫合NS組和LBA組幼鼠左側(cè)眼瞼,建立單眼視覺剝奪性弱視大鼠模型。NS組及LBA組每日分別經(jīng)口給予等量的0.9%NaCl及10mg/kg LBA,正常組自由進(jìn)食,于生后14天、21天、28天、35天、42天、70天分別取材制片,采用Nissl染色測量視皮層17區(qū)神經(jīng)元的數(shù)目和截面積,采用SABC免疫組化法檢測視皮層17區(qū)相應(yīng)區(qū)域Bcl-2和NGF的表達(dá)。 結(jié)果①Nissl染色顯示剝奪性弱視大鼠右側(cè)視皮層分層欠清晰,神經(jīng)元排列相對疏松,細(xì)胞形態(tài)及突起不規(guī)則,Nissl小體表達(dá)有所減少,可見胞核縮小、著色加深。②LBA組大鼠生后28天至70天,右側(cè)視皮層神經(jīng)元的數(shù)量和截面積明顯同齡NS組大鼠的對應(yīng)區(qū)域,差異有顯著性(P0.05)。③LBA組大鼠生后21天至70天右側(cè)視皮層Bcl-2陽性細(xì)胞的數(shù)量和OD值高于同齡NS組并低于正常組大鼠的對應(yīng)區(qū)域,差異有顯著性(P0.05)。④LBA組大鼠生后42天及70天,右側(cè)視皮層17區(qū)NGF陽性細(xì)胞的數(shù)目和OD值高于同齡NS組大鼠的對應(yīng)區(qū)域,差異有顯著性(P0.05)。 結(jié)論①大鼠視覺發(fā)育敏感期,剝奪性弱視可導(dǎo)致視皮層神經(jīng)元細(xì)胞數(shù)目和截面積低于正常組。②LBA對剝奪性弱視大鼠在視覺發(fā)育可塑性的關(guān)鍵期內(nèi)具有一定保護(hù)作用,可作為弱視治療的臨床備選藥物。③LBA對剝奪性弱視大鼠視覺發(fā)育的保護(hù)機(jī)制可能與上調(diào)Bcl-2及NGF的表達(dá)有關(guān),以促進(jìn)神經(jīng)細(xì)胞的營養(yǎng)和生長。
[Abstract]:Objective to establish an animal model of monocular visual deprivation amblyopia and to study the protective effect of Lycium barbarum extract (LBA) on retinal ganglion cells (RGCs) in visual deprived amblyopia rats. Methods Thirty newborn Wistar rats were randomly divided into normal saline group (n = 10), levodopa group (n = 10) and LBA group (n = 10). One week after birth, the left eyelids of all the young rats were sutured with puerperal suture to establish an animal model of unilateral visual deprivation amblyopia. The three groups were given the same amount of NS (0.9% NaCl),) daily. L-dopa (20mg/kg) and LBA (10mg/kg) were fed for 6 weeks. The 30g/L fluorescence gold was injected into the superior colliculus and lateral geniculate bodies of all the rats 5 days before sampling. The changes of RGCs morphology and quantity in retina paving tablets were observed by retrograde labeling with fluorescent gold. Results (1) RGCs labeled in retina paving tablets was yellowish-white, and the cell bodies were various. The density of RGCs in the left eye of 2NS group, L-dopa group and LBA group was significantly lower than that in right eye, while in 2NS group, L-dopa group and LBA group, the density of RGCs in left eye was significantly lower than that in right eye. Compared with NS group, the left eye RGCs density in L-dopa group and LBA group increased significantly (P0.01). The survival rate of RGCs in 4L-dopa group and LBA group was 85.17% 鹵4.42% and 84.31% 鹵5.97%, respectively, which was significantly higher than that in NS group (71.35% 鹵6.34%). There was no significant difference between L-dopa group and LBA group (P0.05). Conclusion 1 during the critical period of visual development plasticity of deprived amblyopia rats, LBA can effectively protect RGCs, from increasing RGCs survival rate. The visual protective effect of 2LBA on RGCs is similar to that of levodopa, a conventional therapy for amblyopia. Objective to establish an animal model of monocular deprivation amblyopia and to study the effects of Lycium medlar extract (LBA) on the expression of Bcl-2 and NGF in the 17 region of visual cortex at different stages of visual development. Methods 60 neonatal Wistar rats were randomly divided into normal group (n = 12) and saline group (n = 24). After 7 days of life, the left eyelids of NS and LBA groups were sutured by puerperal suture, and the model of unilateral visual deprivation amblyopia was established. The NS group and LBA group were given the same amount of 0.9%NaCl and 10mg/kg LBA, respectively daily. The normal group was fed freely. The number and sectional area of neurons in the 17 area of the visual cortex were measured by Nissl staining on the 14th, 21st, 28th, 35th, 42nd and 70th day after birth. SABC immunohistochemical method was used to detect the expression of Bcl-2 and NGF in the 17 region of visual cortex. Results 1Nissl staining showed that the right visual cortex of deprived amblyopia rats was not clearly stratified, the neurons were relatively loose, the shape and process of the cells were irregular, the expression of Nissl corpuscles was decreased, and the nucleus was reduced. From 28 to 70 days after birth, the number and sectional area of the right visual cortex neurons in the 2LBA group were significantly higher than those in the same age NS group. The number and OD value of Bcl-2 positive cells in the right visual cortex in 3LBA group were higher than those in the same age NS group and lower than those in the normal group (P 0.05). There was significant difference (P0.05). The number and OD value of NGF positive cells in the right visual cortex in the 4LBA group were higher than those in the corresponding area of the same age NS group on the 42nd and 70th day after birth (P0.05). Conclusion 1 the number and sectional area of neurons in the visual cortex of the rats with deprived amblyopia were lower than that of the normal group during the sensitive period of visual development. 2LBA has a protective effect on the visual plasticity of the deprived amblyopia rats during the critical period of visual development plasticity. The protective mechanism of 3LBA on visual development of deprived amblyopia rats may be related to the up-regulation of the expression of Bcl-2 and NGF in order to promote the nutrition and growth of nerve cells.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2011
【分類號】：R777.44
本文編號：2325395
[Abstract]:Objective to establish an animal model of monocular visual deprivation amblyopia and to study the protective effect of Lycium barbarum extract (LBA) on retinal ganglion cells (RGCs) in visual deprived amblyopia rats. Methods Thirty newborn Wistar rats were randomly divided into normal saline group (n = 10), levodopa group (n = 10) and LBA group (n = 10). One week after birth, the left eyelids of all the young rats were sutured with puerperal suture to establish an animal model of unilateral visual deprivation amblyopia. The three groups were given the same amount of NS (0.9% NaCl),) daily. L-dopa (20mg/kg) and LBA (10mg/kg) were fed for 6 weeks. The 30g/L fluorescence gold was injected into the superior colliculus and lateral geniculate bodies of all the rats 5 days before sampling. The changes of RGCs morphology and quantity in retina paving tablets were observed by retrograde labeling with fluorescent gold. Results (1) RGCs labeled in retina paving tablets was yellowish-white, and the cell bodies were various. The density of RGCs in the left eye of 2NS group, L-dopa group and LBA group was significantly lower than that in right eye, while in 2NS group, L-dopa group and LBA group, the density of RGCs in left eye was significantly lower than that in right eye. Compared with NS group, the left eye RGCs density in L-dopa group and LBA group increased significantly (P0.01). The survival rate of RGCs in 4L-dopa group and LBA group was 85.17% 鹵4.42% and 84.31% 鹵5.97%, respectively, which was significantly higher than that in NS group (71.35% 鹵6.34%). There was no significant difference between L-dopa group and LBA group (P0.05). Conclusion 1 during the critical period of visual development plasticity of deprived amblyopia rats, LBA can effectively protect RGCs, from increasing RGCs survival rate. The visual protective effect of 2LBA on RGCs is similar to that of levodopa, a conventional therapy for amblyopia. Objective to establish an animal model of monocular deprivation amblyopia and to study the effects of Lycium medlar extract (LBA) on the expression of Bcl-2 and NGF in the 17 region of visual cortex at different stages of visual development. Methods 60 neonatal Wistar rats were randomly divided into normal group (n = 12) and saline group (n = 24). After 7 days of life, the left eyelids of NS and LBA groups were sutured by puerperal suture, and the model of unilateral visual deprivation amblyopia was established. The NS group and LBA group were given the same amount of 0.9%NaCl and 10mg/kg LBA, respectively daily. The normal group was fed freely. The number and sectional area of neurons in the 17 area of the visual cortex were measured by Nissl staining on the 14th, 21st, 28th, 35th, 42nd and 70th day after birth. SABC immunohistochemical method was used to detect the expression of Bcl-2 and NGF in the 17 region of visual cortex. Results 1Nissl staining showed that the right visual cortex of deprived amblyopia rats was not clearly stratified, the neurons were relatively loose, the shape and process of the cells were irregular, the expression of Nissl corpuscles was decreased, and the nucleus was reduced. From 28 to 70 days after birth, the number and sectional area of the right visual cortex neurons in the 2LBA group were significantly higher than those in the same age NS group. The number and OD value of Bcl-2 positive cells in the right visual cortex in 3LBA group were higher than those in the same age NS group and lower than those in the normal group (P 0.05). There was significant difference (P0.05). The number and OD value of NGF positive cells in the right visual cortex in the 4LBA group were higher than those in the corresponding area of the same age NS group on the 42nd and 70th day after birth (P0.05). Conclusion 1 the number and sectional area of neurons in the visual cortex of the rats with deprived amblyopia were lower than that of the normal group during the sensitive period of visual development. 2LBA has a protective effect on the visual plasticity of the deprived amblyopia rats during the critical period of visual development plasticity. The protective mechanism of 3LBA on visual development of deprived amblyopia rats may be related to the up-regulation of the expression of Bcl-2 and NGF in order to promote the nutrition and growth of nerve cells.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2011
【分類號】：R777.44
【引證文獻(xiàn)】
相關(guān)碩士學(xué)位論文 前1條
1 曾亞薇;Caspase-3在形覺剝奪性弱視大鼠視皮層及外側(cè)膝狀體的表達(dá)[D];南華大學(xué);2013年
，本文編號：2325395
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