串珠素反義cDNA對人喉癌裸鼠移植瘤的抑制作用
發(fā)布時間：2018-11-10 15:13
【摘要】：目的:研究串珠素(perlecan)反義cDNA質粒(pAP)對喉癌Hep-2細胞裸鼠移植瘤的影響。方法:應用pAP轉染喉癌Hep-2細胞,建立人喉癌裸鼠移植瘤模型,觀察裸鼠移植瘤的生長速度。分為3組:未轉染的Hep-2細胞組(WT組)、空載體phβApr-neol轉染組(neo組)及pAP轉染組(pAP組)。用RT-PCR和免疫組織化學方法檢測各組裸鼠移植瘤的perlecan mRNA和蛋白質的表達情況。結果:腫瘤生長4周后,WT組和neo組裸鼠移植瘤的平均體積較大,pAP組裸鼠移植瘤的平均體積較小,差異有統(tǒng)計學意義(P0.01)。perlecan mRNA在WT組和neo組高表達,在pAP組低表達,均差異有統(tǒng)計學意義(均P0.05)。perlecan蛋白在WT組和neo組高表達,定位于癌細胞的細胞核和細胞質,在pAP組低表達,均差異有統(tǒng)計學意義(均P0.01)。結論:pAP對喉癌Hep-2細胞裸鼠移植瘤的發(fā)生、發(fā)展有重要影響。
[Abstract]:Aim: to study the effect of antisense cDNA plasmid (pAP) of (perlecan) on transplanted tumor of Hep-2 cells in nude mice. Methods: the transplanted tumor model of human laryngeal carcinoma in nude mice was established by transfection of Hep-2 cells with pAP and the growth rate of transplanted tumor in nude mice was observed. They were divided into three groups: untransfected Hep-2 cells (WT group), empty vector ph 尾 Apr-neol transfection group (neo group) and pAP transfection group (pAP group). RT-PCR and immunohistochemistry were used to detect the expression of perlecan mRNA and protein in xenografts of nude mice. Results: after 4 weeks of tumor growth, the average volume of xenografts in WT group and neo group was larger than that in pAP group, and the difference was statistically significant (P0.01). Perlecan mRNA was highly expressed in WT group and neo group, but low in pAP group. The difference was statistically significant (P0.05) the expression of). Perlecan protein was high in WT group and neo group, located in the nucleus and cytoplasm of cancer cells, and low in pAP group (all P0.01). Conclusion: pAP plays an important role in the development and development of transplanted tumor of Hep-2 cells in nude mice.
【作者單位】： 河南科技大學第三附屬醫(yī)院洛陽東方醫(yī)院耳鼻咽喉科;首都醫(yī)科大學附屬北京同仁醫(yī)院耳鼻咽喉頭頸外科;華中科技大學同濟醫(yī)學院附屬協(xié)和醫(yī)院耳鼻咽喉科;
【分類號】：R739.65
本文編號：2322802
[Abstract]:Aim: to study the effect of antisense cDNA plasmid (pAP) of (perlecan) on transplanted tumor of Hep-2 cells in nude mice. Methods: the transplanted tumor model of human laryngeal carcinoma in nude mice was established by transfection of Hep-2 cells with pAP and the growth rate of transplanted tumor in nude mice was observed. They were divided into three groups: untransfected Hep-2 cells (WT group), empty vector ph 尾 Apr-neol transfection group (neo group) and pAP transfection group (pAP group). RT-PCR and immunohistochemistry were used to detect the expression of perlecan mRNA and protein in xenografts of nude mice. Results: after 4 weeks of tumor growth, the average volume of xenografts in WT group and neo group was larger than that in pAP group, and the difference was statistically significant (P0.01). Perlecan mRNA was highly expressed in WT group and neo group, but low in pAP group. The difference was statistically significant (P0.05) the expression of). Perlecan protein was high in WT group and neo group, located in the nucleus and cytoplasm of cancer cells, and low in pAP group (all P0.01). Conclusion: pAP plays an important role in the development and development of transplanted tumor of Hep-2 cells in nude mice.
【作者單位】： 河南科技大學第三附屬醫(yī)院洛陽東方醫(yī)院耳鼻咽喉科;首都醫(yī)科大學附屬北京同仁醫(yī)院耳鼻咽喉頭頸外科;華中科技大學同濟醫(yī)學院附屬協(xié)和醫(yī)院耳鼻咽喉科;
【分類號】：R739.65
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