【摘要】：目的:研究串珠素(perlecan)反義cDNA質(zhì)粒(pAP)對(duì)喉癌Hep-2細(xì)胞裸鼠移植瘤的影響。方法:應(yīng)用pAP轉(zhuǎn)染喉癌Hep-2細(xì)胞,建立人喉癌裸鼠移植瘤模型,觀察裸鼠移植瘤的生長(zhǎng)速度。分為3組:未轉(zhuǎn)染的Hep-2細(xì)胞組(WT組)、空載體phβApr-neol轉(zhuǎn)染組(neo組)及pAP轉(zhuǎn)染組(pAP組)。用RT-PCR和免疫組織化學(xué)方法檢測(cè)各組裸鼠移植瘤的perlecan mRNA和蛋白質(zhì)的表達(dá)情況。結(jié)果:腫瘤生長(zhǎng)4周后,WT組和neo組裸鼠移植瘤的平均體積較大,pAP組裸鼠移植瘤的平均體積較小,差異有統(tǒng)計(jì)學(xué)意義(P0.01)。perlecan mRNA在WT組和neo組高表達(dá),在pAP組低表達(dá),均差異有統(tǒng)計(jì)學(xué)意義(均P0.05)。perlecan蛋白在WT組和neo組高表達(dá),定位于癌細(xì)胞的細(xì)胞核和細(xì)胞質(zhì),在pAP組低表達(dá),均差異有統(tǒng)計(jì)學(xué)意義(均P0.01)。結(jié)論:pAP對(duì)喉癌Hep-2細(xì)胞裸鼠移植瘤的發(fā)生、發(fā)展有重要影響。
[Abstract]:Aim: to study the effect of antisense cDNA plasmid (pAP) of (perlecan) on transplanted tumor of Hep-2 cells in nude mice. Methods: the transplanted tumor model of human laryngeal carcinoma in nude mice was established by transfection of Hep-2 cells with pAP and the growth rate of transplanted tumor in nude mice was observed. They were divided into three groups: untransfected Hep-2 cells (WT group), empty vector ph 尾 Apr-neol transfection group (neo group) and pAP transfection group (pAP group). RT-PCR and immunohistochemistry were used to detect the expression of perlecan mRNA and protein in xenografts of nude mice. Results: after 4 weeks of tumor growth, the average volume of xenografts in WT group and neo group was larger than that in pAP group, and the difference was statistically significant (P0.01). Perlecan mRNA was highly expressed in WT group and neo group, but low in pAP group. The difference was statistically significant (P0.05) the expression of). Perlecan protein was high in WT group and neo group, located in the nucleus and cytoplasm of cancer cells, and low in pAP group (all P0.01). Conclusion: pAP plays an important role in the development and development of transplanted tumor of Hep-2 cells in nude mice.
【作者單位】： 河南科技大學(xué)第三附屬醫(yī)院洛陽(yáng)東方醫(yī)院耳鼻咽喉科;首都醫(yī)科大學(xué)附屬北京同仁醫(yī)院耳鼻咽喉頭頸外科;華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬協(xié)和醫(yī)院耳鼻咽喉科;
【分類號(hào)】：R739.65

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