【摘要】： 目的：檢測Ezrin和RhoA蛋白各自在不同臨床分期鼻咽癌(Nasopharyngeal Carcinoma, NPC)組織中的表達,探討二者在NPC發(fā)生和發(fā)展中的作用以及它們在其中的相互關系。為NPC浸潤轉移的機制提供新的理論依據(jù)。為NPC的早期診斷、臨床防治及預后判斷提供新的有價值的指標。 方法： 1.選取有完整臨床資料及隨訪資料的150例NPC病例石蠟包埋組織標本作為實驗組,選取20例鼻咽粘膜慢性炎石蠟包埋組織作為對照。 2.應用免疫組化Supervision二步法分別檢測NPC和對照組組織中Ezrin和RhoA蛋白的表達情況。 3.采用SPSS13.0統(tǒng)計軟件進行數(shù)據(jù)處理。用x2檢驗、Spearman等級相關分析法分析Ezrin和RhoA蛋白表達與NPC患者臨床病理特征之間的關系；用Kaplan-Meier法進行單因素生存分析,log-rank法進行樣本間生存率曲線差異檢驗。 結果： 1. Ezrin與RhoA蛋白在NPC組織中的陽性表達率分別為74.7%和62.7%,在對照組組織中基本不表達。兩組差異都有非常顯著統(tǒng)計學意義(均為PO.001)。 2. Ezrin與RhoA蛋白陽性表達率在NPC臨床TNM分期的T1、T2、T3、T4分別為：29.4%、65.5%、92.3%、88.5%和17.6%、52.7%、78.8%、80.8%,除T3與T4期,各T分期之間比較均有顯著或非常顯著統(tǒng)計學意義(P0.05或P0.001)；在N0、N1、N2、N3分別為53.8%、71.4%、79.6%、87.9%和46.2%、54.8%、65.3%、81.8%,Ezrin蛋白表達在N0與N2,N0與N3分期之間有顯著差異(P0.05),RhoA蛋白表達在N0與N1,N0與N3以及N1與N3分期之間有顯著差異(P0.05)；在M0與M1分別為72.5%、94.7%和60.3%、84.2%, M0與M1之間比較均有顯著統(tǒng)計學意義(均為P0.05)。 3. Ezrin與RhoA蛋白陽性表達率在臨床分期Ⅰ、Ⅱ、Ⅲ、Ⅳ期中分別為50%、40%、80.7%、86.9%和0%、26.7%、64.9%、80.3%,兩指標各期之間均有非常顯著統(tǒng)計學意義(均為P0.001)。 4.NPC組織中Ezrin與RhoA兩者表達呈顯著正相關(Rs=0.486,P0.001)。 5. Ezrin和RhoA蛋白表達陽性的患者,治療后1年、3年及5年生存率分別為：75%,55%,33%和84%,60%,33%,均低于陰性表達100%,92%,78%和93%,71%,63%。均有顯著性差異(x2=12.943,P=0.000及x2=9.869,P=0.0020.05)。 結論： 1. Ezrin和RhoA蛋白表達均與NPC的發(fā)生可能有關,但更可能是Ezrin和RhoA蛋白表達的NPC細胞本身具有浸潤轉移潛能。 2. Ezrin和RhoA蛋白表達均參與了NPC的早期局部浸潤轉移、淋巴結轉移和遠處器官轉移等過程,并很有可能是重要的參與因子。 3. Ezrin與RhoA蛋白表達呈顯著正相關,進一步間接證明了兩者在結構、功能上和基因表達等方面有著密切聯(lián)系,同時表明它們很可能為NPC浸潤轉移的共同或協(xié)同促進因子。 4. Ezrin和RhoA與NPC的預后有關。Ezrin或RhoA陽性表達的NPC患者平均生存率低,特別是Ezrin和RhoA同時陽性表達者較Ezrin和RhoA同時陰性表達者生存期短,預后差。
[Abstract]:Aim: to detect the expression of Ezrin and RhoA proteins in (Nasopharyngeal Carcinoma, NPC) tissues of nasopharyngeal carcinoma at different clinical stages and to explore their roles in the pathogenesis and development of NPC and their relationship. It provides a new theoretical basis for the mechanism of NPC invasion and metastasis. To provide a new valuable index for early diagnosis, clinical prevention and prognosis of NPC. Methods: 1. Paraffin embedded tissue specimens of 150 cases of NPC with complete clinical data and follow-up data were selected as experimental group and 20 cases of nasopharyngeal mucosa chronic inflammation paraffin embedded tissue as control group. 2. The expression of Ezrin and RhoA in NPC and control group was detected by immunohistochemical Supervision two-step method. 3. SPSS13.0 statistical software is used for data processing. The relationship between the expression of Ezrin and RhoA protein and the clinicopathological characteristics of NPC patients was analyzed by x2 test and Spearman rank correlation analysis. Results: 1. The positive expression rates of Ezrin and RhoA in NPC were 74.7% and 62.7%, respectively. The differences between the two groups were statistically significant (both PO.001). 2. The positive expression rate of Ezrin and RhoA protein in clinical TNM stage of NPC was 29.4% and 65.5%, respectively. The positive expression rate of Ezrin and RhoA protein was 88.8% and 52.7%, respectively, except T 3 and T 4, respectively, with the exception of T 3 and T 4, and T 3, T 4, T 3 and T 4, respectively. There were significant or very significant statistical differences among T stages (P0.05 or P0.001). At N0, N1, N2N3 was 53.81.41, and 79.6% and 46.2%, 54.8and 65.3%, respectively, and the Ezrin protein was expressed in N0 and N2, and the Ezrin protein was expressed in N0 and N2. There was significant difference between N0 and N3 stages (P0.05). The expression of), RhoA protein was significantly different between N0 and N1N0 and N3 and N1 and N3 stages (P0.05). M0 and M1 were 72.5% and 60.3%, respectively, and there were significant differences between M0 and M1 (P0.05). 3. The positive expression rates of Ezrin and RhoA protein in clinical stages 鈪，

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