【摘要】：目的:觀察c-jun、brg1在喉鱗癌的表達(dá)及意義,并分析二者與喉鱗癌的臨床病理各因素的關(guān)系,探討c-jun、brg1兩者在喉鱗癌患者中表達(dá)的相關(guān)性,為喉鱗癌的早期診斷提供可供參考的依據(jù)。 方法:用免疫組織化學(xué)染色法檢測(cè)45例喉鱗癌組織、10例癌旁正常組織、10例聲帶息肉中c-jun、brg1表達(dá),比較喉鱗癌組織、癌旁正常組織及聲帶息肉中c-jun、brg1表達(dá)的差異性。觀察喉鱗癌組織c-jun、brg-1表達(dá)與臨床病理各因素:年齡、性別、病理分級(jí)、臨床分期、淋巴結(jié)轉(zhuǎn)移、原發(fā)部位等之間的相互關(guān)系。 結(jié)果: 1、c-jun在喉鱗癌中的陽(yáng)性表達(dá)率明顯高于癌旁組織及聲帶息肉。c-jun在喉鱗癌與癌旁組織、喉鱗癌與聲帶息肉中的陽(yáng)性表達(dá)率比較,有顯著性差異(P0.05),而在癌旁組織與聲帶息肉中的陽(yáng)性表達(dá)率比較,無(wú)差異性(P0.05)。 c-jun在喉鱗癌患者中,發(fā)現(xiàn)年齡、性別及原發(fā)部位組間中陽(yáng)性表達(dá)率比較,無(wú)統(tǒng)計(jì)學(xué)意義(p0.05),而在病理分級(jí),臨床分期及有無(wú)淋巴結(jié)轉(zhuǎn)移組間中陽(yáng)性表達(dá)率有統(tǒng)計(jì)學(xué)意義(p0.05)。c-jun在高、中及低分化喉鱗癌中的陽(yáng)性率分別為89.5%,80.0%及54.5%,其陽(yáng)性表達(dá)率隨喉鱗癌病理分級(jí)的降低而逐漸下降,且高、中分化,中、低分化組間比較,無(wú)統(tǒng)計(jì)學(xué)意義(p0.05),高、低分化組間比較差異有顯著性(p0.05)。 2、brg1在癌旁組織中的陽(yáng)性表達(dá)率明顯高于喉鱗癌和聲帶息肉。brg1在喉鱗癌與癌旁組織,癌旁組織與聲帶息肉中的陽(yáng)性表達(dá)率比較,有顯著性差異(P0.05),在喉鱗癌與聲帶息肉中陽(yáng)性表達(dá)率比較,無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。 brg1在喉鱗癌患者中,發(fā)現(xiàn)年齡、性別及原發(fā)部位組間中陽(yáng)性表達(dá)率比較,無(wú)統(tǒng)計(jì)學(xué)意義(p0.05),而在病理分級(jí),臨床分期及有無(wú)淋巴結(jié)轉(zhuǎn)移組間中陽(yáng)性表達(dá)率有統(tǒng)計(jì)學(xué)意義(p0.05)。brg1在高、中及低分化喉鱗癌中的陽(yáng)性率分別為73.7%,40.0%及18.2%,其陽(yáng)性表達(dá)率隨喉鱗癌病理分級(jí)的降低而逐漸下降,且中、低分化組間比較,無(wú)統(tǒng)計(jì)學(xué)意義(p0.05),高、中分化,高、低分化組間比較差異有顯著性(p0.05)。 3、c-jun、brg1兩種蛋白在喉鱗癌中的表達(dá)無(wú)相關(guān)性r=0.5874(p0.05)。 結(jié)論: 1、c-jun的表達(dá)陽(yáng)性率在喉鱗癌組織顯著高于癌旁組織和聲帶息肉,而brg1的表達(dá)陽(yáng)性率在癌旁組織中明顯高于喉鱗癌及聲帶息肉,提示二者與喉鱗癌的發(fā)生、發(fā)展密切相關(guān)。 2、c-jun和brg1的表達(dá)與喉鱗癌的病理分級(jí)、臨床分期、淋巴結(jié)的有無(wú)轉(zhuǎn)移相關(guān),而與年齡、性別及原發(fā)部位無(wú)關(guān),c-jun可能在喉鱗癌的發(fā)生、發(fā)展中起促進(jìn)作用,而brg1正好相反,c-jun及brg1的表達(dá)可能提示組織的惡性程度。 3、c-jun和brg1在喉鱗癌中表達(dá)呈無(wú)相關(guān)性,提示二者可能在多種因素介導(dǎo)下參與喉鱗癌的發(fā)生及發(fā)展。
[Abstract]:Objective: to observe the expression and significance of c-junn brg1 in laryngeal squamous cell carcinoma (LSCC), and to analyze the relationship between c-junbbrg1 and clinicopathologic factors of laryngeal squamous cell carcinoma (LSCC), and to explore the correlation between c-junnbr g1 and c-junbbrg1 expression in laryngeal squamous cell carcinoma (LSCC) so as to provide reference for early diagnosis of laryngeal squamous cell carcinoma (SCC). Methods: the expression of c-junn brg1 in 45 cases of laryngeal squamous cell carcinoma (LSCC), 10 cases of adjacent normal tissues and 10 cases of vocal cord polyps were detected by immunohistochemical staining. The difference of c-junbbrg1 expression in laryngeal squamous cell carcinoma (LSCC), adjacent normal tissues and vocal cord polyps was compared. To observe the relationship between c-junn brg-1 expression and clinicopathological factors: age, sex, pathological grade, clinical stage, lymph node metastasis and primary location of laryngeal squamous cell carcinoma (LSCC). Results: 1 the positive expression rate of c-jun in laryngeal squamous cell carcinoma was significantly higher than that in paracancerous tissue and vocal cord polyp. The positive expression rate of c-jun in laryngeal squamous cell carcinoma and paracancerous tissue, laryngeal squamous cell carcinoma and vocal cord polyp was higher than that in laryngeal squamous cell carcinoma and vocal cord polyp. There was significant difference (P0.05), but there was no difference between the expression rate of c-jun in adjacent tissues and vocal cord polyps (P0.05). The positive expression rate of c-jun in laryngeal squamous cell carcinoma patients was found in age, sex and primary location group. There was no statistical significance (p0. 05), but the positive expression rate in pathological grade, clinical stage and lymph node metastasis group was statistically significant (p0. 05). The expression rate of c-jun was higher in the group with or without lymph node metastasis (p0. 05). The positive rates of middle and low differentiation laryngeal squamous cell carcinoma were 80.0% and 54.5%, respectively. The positive expression rate decreased gradually with the decrease of pathological grade of laryngeal squamous cell carcinoma. There was significant difference between the low differentiation groups (p0. 05). 2the positive expression rate of BRg1 in adjacent tissues was significantly higher than that in laryngeal squamous cell carcinoma and vocal cord polyp. The positive expression rate of brg1 in laryngeal squamous cell carcinoma and paracancerous tissue, paracancerous tissue and vocal cord polyp was higher than that in laryngeal squamous cell carcinoma and vocal cord polyp. There was significant difference (P0.05). There was no significant difference between the positive expression rate of brg1 in laryngeal squamous cell carcinoma and vocal cord polyp (P0.05). There was no statistical significance (p0. 05), but the positive expression rate in pathological grade, clinical stage and lymph node metastasis group was statistically significant (p0. 05). The expression rate of brg1 was higher in the group with or without lymph node metastasis (p0. 05). The positive rates of middle and low differentiated laryngeal squamous cell carcinoma were 40.0% and 18.2%, respectively. The positive expression rate decreased gradually with the decrease of pathological grade of laryngeal squamous cell carcinoma, and there was no significant difference between middle and low differentiation groups (p0.05). The expression of c-junbbrg1 protein in laryngeal squamous cell carcinoma (LSCC) was not correlated with r-0.5874 (p0.05). Conclusion: 1 the positive rate of c-jun expression in laryngeal squamous cell carcinoma is significantly higher than that in adjacent laryngeal squamous cell carcinoma and vocal cord polyp, while the positive rate of brg1 in adjacent laryngeal squamous cell carcinoma and vocal cord polyp is significantly higher than that in adjacent laryngeal squamous cell carcinoma and vocal cord polyp, suggesting that the expression of c-jun in laryngeal squamous cell carcinoma is associated with laryngeal squamous cell carcinoma. 2the expression of c-jun and brg1 was correlated with the pathological grade, clinical stage and lymph node metastasis of laryngeal squamous cell carcinoma, but not with age, sex and primary location. C-jun may occur in laryngeal squamous cell carcinoma. The expression of c-jun and brg1 may indicate the degree of malignancy of tissue. 3The expression of c-jun and brg1 in laryngeal squamous cell carcinoma has no correlation. These results suggest that they may be involved in the occurrence and development of laryngeal squamous cell carcinoma (LSCC).
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類(lèi)號(hào)】：R739.65

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本文編號(hào)：2285185