【摘要】： 目的:探討喉癌患者手術(shù)前、術(shù)后第1天、術(shù)后第5天、術(shù)后第10天外周血游離K-ras基因的突變情況,及與喉癌患者TNM臨床分類、臨床分期、病理分級等臨床因素之間的關(guān)系,為喉癌患者術(shù)前進(jìn)行無創(chuàng)性檢查,鑒別良惡性及監(jiān)測預(yù)后提供理論依據(jù),進(jìn)而為喉癌的早期診斷及臨床治療提供理論依據(jù)。 方法:采集29例喉癌患者外周血,包括手術(shù)前、術(shù)后第1天、術(shù)后第5天、術(shù)后第10天外周血。取10例正常自愿獻(xiàn)血者的外周血作為正常對照。應(yīng)用液相芯片和突變富集PCR技術(shù)檢測每份標(biāo)本中K-ras基因12、13位編碼子的點(diǎn)突變情況。 結(jié)果:游離DNA K-ras基因突變率,喉癌術(shù)前組與正常對照組比較,術(shù)后組與正常對照組比較,術(shù)前組與術(shù)后組比較,無顯著差異(P 0.05);隨喉癌的TNM臨床分類、臨床分期、病理分級的上升有增高趨勢,各臨床因素內(nèi)比較無顯著差異(Fisher精確概率檢驗(yàn)值0.05). 結(jié)論:游離DNA K-ras基因的點(diǎn)突變檢測尚不能為喉癌提供臨床指導(dǎo);Luminex液相芯片技術(shù)及游離DNA檢測在喉癌臨床中有一定的應(yīng)用前景。
[Abstract]:Objective: to investigate the mutation of K-ras gene in peripheral blood of laryngeal cancer patients before operation, 1 day after operation, 5 days after operation and 10 days after operation, and the relationship between the mutation of K-ras gene and clinical classification, clinical stage, pathological grade and other clinical factors in patients with laryngeal carcinoma. It provides theoretical basis for preoperative noninvasive examination, differential diagnosis of benign and malignant tumor and monitoring prognosis, and then provides theoretical basis for early diagnosis and clinical treatment of laryngeal carcinoma. Methods: the peripheral blood of 29 patients with laryngeal carcinoma was collected, including the peripheral blood before operation, 1 day after operation, 5 days after operation and 10 days after operation. The peripheral blood of 10 normal voluntary blood donors was taken as normal control. The point mutation of K-ras gene 1213 in each sample was detected by liquid phase microarray and mutagenic enrichment PCR. Results: there was no significant difference in the mutation rate of free DNA K-ras gene between preoperative group and normal control group (P 0.05), and with the TNM clinical classification and clinical stage of laryngeal carcinoma, there was no significant difference between preoperative group and normal control group (P 0.05). The pathological grade increased with no significant difference among the clinical factors (Fisher accurate probability test 0.05). Conclusion: the point mutation detection of free DNA K-ras gene can not provide clinical guidance for laryngeal carcinoma. Luminex liquid-phase microarray technique and free DNA detection have a certain application prospect in laryngeal carcinoma.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R739.65

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本文編號：2121775